中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
5期
475-480
,共6页
庞杏林%侯配斌%高源%徐丽%任红宇%朱兵清%周海健%邵祝军
龐杏林%侯配斌%高源%徐麗%任紅宇%硃兵清%週海健%邵祝軍
방행림%후배빈%고원%서려%임홍우%주병청%주해건%소축군
脑膜炎奈瑟菌%插入序列IS1301%脉冲场凝胶电泳%核酸印迹杂交
腦膜炎奈瑟菌%插入序列IS1301%脈遲場凝膠電泳%覈痠印跡雜交
뇌막염내슬균%삽입서렬IS1301%맥충장응효전영%핵산인적잡교
Neisseria meningitidis%Insertion sequence 1301%Pulse field gel electrophoresis%Southern blot
目的 研究中国脑膜炎奈瑟菌(Nm)中插入序列IS1301的分布与分子流行病学特征.方法 设计IS1301引物,应用PCR方法检测2007年1月至2008年10月收集全国16个省、市、自治区219株Nm菌株的IS1301,对IS1301扩增产物进行DNA测序和序列比对,PCR扩增IS1301阳性菌株进行脉冲场凝胶电泳(PFGE)分析及核酸印迹杂交,研究不同Nm菌株中IS1301的分布特征.结果 219株Nm菌株中,34株携带IS1301(15.53%),A、B、C群和不可分群的Nm菌株IS1301阳性率分别为11.11%、20.75%、6.17%和28.57%;IS1301扩增产物测序结果与GenBank登记编号ZA9092.1的Nm菌株序列相似度为94%～100%,进化树分析可分为两簇;C群Nm菌株与其他血清群菌株IS1301序列存在较大差别;研究的Nm菌株基因组中至少存在6～17个IS1301拷贝,相同PFGE带型Nm菌株所携带的IS1301拷贝数具有多态性特征.结论 IS1301在中国Nm菌株中的分布具有遗传多态性.
目的 研究中國腦膜炎奈瑟菌(Nm)中插入序列IS1301的分佈與分子流行病學特徵.方法 設計IS1301引物,應用PCR方法檢測2007年1月至2008年10月收集全國16箇省、市、自治區219株Nm菌株的IS1301,對IS1301擴增產物進行DNA測序和序列比對,PCR擴增IS1301暘性菌株進行脈遲場凝膠電泳(PFGE)分析及覈痠印跡雜交,研究不同Nm菌株中IS1301的分佈特徵.結果 219株Nm菌株中,34株攜帶IS1301(15.53%),A、B、C群和不可分群的Nm菌株IS1301暘性率分彆為11.11%、20.75%、6.17%和28.57%;IS1301擴增產物測序結果與GenBank登記編號ZA9092.1的Nm菌株序列相似度為94%～100%,進化樹分析可分為兩簇;C群Nm菌株與其他血清群菌株IS1301序列存在較大差彆;研究的Nm菌株基因組中至少存在6～17箇IS1301拷貝,相同PFGE帶型Nm菌株所攜帶的IS1301拷貝數具有多態性特徵.結論 IS1301在中國Nm菌株中的分佈具有遺傳多態性.
목적 연구중국뇌막염내슬균(Nm)중삽입서렬IS1301적분포여분자류행병학특정.방법 설계IS1301인물,응용PCR방법검측2007년1월지2008년10월수집전국16개성、시、자치구219주Nm균주적IS1301,대IS1301확증산물진행DNA측서화서렬비대,PCR확증IS1301양성균주진행맥충장응효전영(PFGE)분석급핵산인적잡교,연구불동Nm균주중IS1301적분포특정.결과 219주Nm균주중,34주휴대IS1301(15.53%),A、B、C군화불가분군적Nm균주IS1301양성솔분별위11.11%、20.75%、6.17%화28.57%;IS1301확증산물측서결과여GenBank등기편호ZA9092.1적Nm균주서렬상사도위94%～100%,진화수분석가분위량족;C군Nm균주여기타혈청군균주IS1301서렬존재교대차별;연구적Nm균주기인조중지소존재6～17개IS1301고패,상동PFGE대형Nm균주소휴대적IS1301고패수구유다태성특정.결론 IS1301재중국Nm균주중적분포구유유전다태성.
Objective To research the distribution and molecular epidemiology of insertion sequence IS1301 in Neisseria (N.) meningitidis strains in China, so as to provide scientific and available evidence for a new method of genotyping in N.meningitidis strains with IS1301. Methods Examined the IS1301 by PCR in 219 N.meningitidis strains from 16 provinces and 3 cities during 2007 and 2008 in China, productions of amplification were sent for sequencing. The positive N.meningitidis strains were analyzed by pulse field gel electrophoresis (PFGE) and nucleic acid blotting hybridization(Southern blot) by electrophoresis. Results The positive rates with IS1301 were 15.53%, 11.11%, 20.75%, 6.17% and 28.57% for four serotypes (A, B, C, N) respectively. The sequence comparability between the amplification productions and No.Z49092.1 N.meningitidis which registered in GenBank was 94%-100%. There were two types of clusters devided by cladogram analysis. There appeared large IS1301 sequence difference between the serotype C and others. The number of IS1301 replica ranged from 6-17 per strain at least. The number of IS1301 replica changed in the same type of PFGE N.meningitidis respectively. Conclusion Typing by IS1301 combined with PFGE could comprehend the homology and genetic polymorphism of N.meningitidis epidemic strains at the molecular level.
