CAJ | 학술논문

目的观察微弧氧化锶磷灰石(strontium-substituted hydroxyapatite,Sr-HA)涂层钛种植体的成骨活性,探讨锶元素掺入羟基磷灰石(hydroxyapatite,HA)涂层后对骨结合的影响.方法分别对实验组(Sr-HA涂层种植体)和对照组(HA涂层种植体)的表面形貌进行扫描电镜观察,并采用X线衍射仪分析膜层相组成.将两组种植体各12枚植入新西兰兔双侧股骨髁下外侧、胫骨结节处,术后对实验动物进行多荧光序列标记,饲养4周和12周取材,采用组织学观察、种植-骨界面线扫描分析评价涂层的骨结合能力.结果种植体微弧氧化膜层均呈现多级孔洞状结构;X射线衍射分析显示,锶元素的掺入引起HA的衍射峰向小角度方向偏移,晶格间距增大,稳定性下降;多荧光序列标记显示,Sr-HA涂层早期即表现出成骨活性,实验组骨矿化沉积率[(4.75±0.46)μm/d]显著高于对照组[(3.21±0.44)μm/d];组织染色及能谱分析显示,骨种植界面上有生物类骨磷灰石层形成,12周时该磷灰石层转化为成熟的骨组织并与涂层形成紧密的骨结合.结论锶元素的掺入可提高Sr-HA涂层的生物学活性,加速其表面生物类骨磷灰石层的形成,增强膜层的成骨能力,促进膜层与骨组织的结合.
목적 관찰미호양화송린회석(strontium-substituted hydroxyapatite,Sr-HA)도층태충식체적성골활성,탐토송원소참입간기린회석(hydroxyapatite,HA)도층후대골결합적영향.방법 분별대실험조(Sr-HA도층충식체)화대조조(HA도층충식체)적표면형모진행소묘전경관찰,병채용X선연사의분석막층상조성.장량조충식체각12매식입신서란토쌍측고골과하외측、경골결절처,술후대실험동물진행다형광서렬표기,사양4주화12주취재,채용조직학관찰、충식-골계면선소묘분석평개도층적골결합능력.결과 충식체미호양화막층균정현다급공동상결구;X사선연사분석현시,송원소적참입인기HA적연사봉향소각도방향편이,정격간거증대,은정성하강;다형광서렬표기현시,Sr-HA도층조기즉표현출성골활성,실험조골광화침적솔[(4.75±0.46)μm/d]현저고우대조조[(3.21±0.44)μm/d];조직염색급능보분석현시,골충식계면상유생물류골린회석층형성,12주시해린회석층전화위성숙적골조직병여도층형성긴밀적골결합.결론 송원소적참입가제고Sr-HA도층적생물학활성,가속기표면생물류골린회석층적형성,증강막층적성골능력,촉진막층여골조직적결합.
Objective To explore the osteogenic activity of a micro-arc oxidation (MAO)-treated strontium (St)-substituted hydroxyapatite (St-HA) coating developed to enhance the osseointegration of titanium dental implants, and to investigate the strengthening mechanisms of bone bonding of crystalline hydroxyapatite(HA) with incorporation of strontium in vivo. Methods The morphology and phase component of the oxidized film of Sr-HA and HA coated implants were examined by SEM and X-raydiffraction(XRD). Then, twenty-four imlpants were inserted into the metaphysis of rabbits tibias and femurs using polyfluorochrome sequential labeling. Four and 12 weeks following the surgery, the morphology and chemical composition of the bone-implant interfaces were evaluated by histological examination and energy-dispersive X-ray. Results The XRD patterns showed that diffraction peaks of HA shift to lower 20 values with Sr-addition, which resulted in decreases in lattice energy and then erystallinity. St-HA coating presented a microporous structure in the SEM observation. Meanwhile, Sr-HA coating exhibited osteogenic activity at the early stage of bone healing period and new bone mineral apposition ratio [(4. 75 ±0.46) μm/d]was significantly higher than that of the control group[(3.21 +0.44) μm/d]. An apatite layer was observed at the interface of bone-Sr-HA coating in light microscopy observation and energy-dispersive X-ray analysis. Then the apatite layer was precipitated and formed new bone which became mature bone and bonded tightly to the Sr-HA. Conclusions Strontium-substituted hydroxyapatite coating shows high biological activity, which can accelerate the formation of apatite layer, hence the osteogenic ability.