CAJ | 학술논문

目的探讨磁共振氢质子波谱(1H-MRS)评价大鼠原位肝移植(OLT)热缺血模型肝细胞再生能力的意义,以及热缺血对于移植肝再生能力的影响. 方法以实验组热缺血10min的大鼠肝移植模型和对照组无热缺血大鼠肝移植模型各30只为研究对象,移植术后分6个时间点(6 h、1 d、3 d、7 d、14 d、30 d)对每只大鼠行肝脏常规T1WI、T2WI成像及氢质子波谱扫描.扫描后取肝脏,测定肝细胞的细胞增殖核抗原(PCNA)表达,同时检测肝酶代谢水平. 结果术后5个时间点实验组的肝细胞PCNA阳性率、胆碱峰与水峰的峰高比值均高于对照组,实验组和对照组胆碱峰/水峰峰高比值均与相应PCNA的阳性率呈显著正相关(r实验=0.819,P＜0.01;r对照=0.543,P＜0.01).实验组和对照组的血清ALT、AST术后明显升高,尤以术后6 h～3 d最为显著,实验组的ALT、AST明显高于对照组. 结论热缺血再灌注损伤对于移植后肝细胞的再生能力有明显的影响,1H-MRS的胆碱峰可以无创伤地评价移植肝的肝细胞再生能力.
목적 탐토자공진경질자파보(1H-MRS)평개대서원위간이식(OLT)열결혈모형간세포재생능력적의의,이급열결혈대우이식간재생능력적영향. 방법 이실험조열결혈10min적대서간이식모형화대조조무열결혈대서간이식모형각30지위연구대상,이식술후분6개시간점(6 h、1 d、3 d、7 d、14 d、30 d)대매지대서행간장상규T1WI、T2WI성상급경질자파보소묘.소묘후취간장,측정간세포적세포증식핵항원(PCNA)표체,동시검측간매대사수평. 결과 술후5개시간점실험조적간세포PCNA양성솔、담감봉여수봉적봉고비치균고우대조조,실험조화대조조담감봉/수봉봉고비치균여상응PCNA적양성솔정현저정상관(r실험=0.819,P＜0.01;r대조=0.543,P＜0.01).실험조화대조조적혈청ALT、AST술후명현승고,우이술후6 h～3 d최위현저,실험조적ALT、AST명현고우대조조. 결론 열결혈재관주손상대우이식후간세포적재생능력유명현적영향,1H-MRS적담감봉가이무창상지평개이식간적간세포재생능력.
Objective To investigate the prognostic value of proton magnetic resonance spectroscopy(1H-MRS)in the study of warm ischemia reperfusion injury to the regeneration of hepatic cells of the livers following their orthotopic transplantation in rats.Methods A rat orthotopic liver transplantation (OLT)model with warm ischemia,the experimental group,was established and the same was done with a control group but without warm ischemia of the livers.They were studied at 6 time points(6 hours,1,3,7,14,and 30 days after OLT).All rats took axial T1 weighted and T2 weighted imaging scans and 1H MR spectroscopies.Results The positive rate of proliferating cell nuclear antigen(PCNA)and the mean peak choline/water ratio in the experimental group were significantly higher than those in the control group and the peak choline/water ratio had a positive correlation with the positive rate of PCNA.Serum ALT and AST increased significantly after OLT,especially during the 6 hour to 3day period.The levels of ALT and AST were markedly higher in the experimental group compared to the control group.Conclusion Warm ischemia reperfusion injury of OLT has a significant effect on the regeneration of hepatic cells,and the choline peak of 1H-MRS can be used to evaluate the regeneration of hepatic cells non-invasively.