国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2011年
6期
393-396
,共4页
脂多糖%巨噬细胞%衰老%IL-6
脂多糖%巨噬細胞%衰老%IL-6
지다당%거서세포%쇠로%IL-6
Lipopolysaccharide%Macrophage%Senescence%IL-6
目的 探讨脂多糖(LPS)对年轻和老龄小鼠腹腔巨噬细胞体内外表达炎性细胞因子IL-6的影响.方法 年轻(2个月)和年老(18个月)小鼠分别腹腔注射LPS,对照组注射PBS,24h后处死小鼠,分离腹腔巨噬细胞,收集细胞,real time PCR检测IL-6的表达水平.未经LPS刺激的小鼠腹腔巨噬细胞,体外用LPS处理24h,PBS作为对照,ELISA方法检测细胞上清中的IL-6浓度.结果 自然状态下衰老小鼠腹腔巨噬细胞中IL-6的表达水平高于年轻小鼠(t=-18.538,P＜0.01).腹腔注射LPS后,IL-6在两个年龄组小鼠腹腔巨噬细胞中的表达均显著上升,但在衰老小鼠中的表达明显低于年轻小鼠(t =6.124,P＜0.01),差异有统计学意义.分离出来的小鼠腹腔巨噬细胞,在没有及有LPS刺激的情况下,衰老小鼠体外培养的细胞分泌炎症因子IL-6的功能与年轻小鼠相比均显著下降(t值分别为12.329和5.876,P值均＜0.05),差异均有统计学意义.结论 衰老小鼠腹腔巨噬细胞在体内持续表达高水平的IL-6,接受LPS刺激后,细胞诱导合成IL-6的能力明显衰退.腹腔巨噬细胞脱离体内环境后,衰老小鼠在体外表现出明显的免疫功能衰退现象,分泌细胞因子IL-6的能力相对于年轻组明显减弱.
目的 探討脂多糖(LPS)對年輕和老齡小鼠腹腔巨噬細胞體內外錶達炎性細胞因子IL-6的影響.方法 年輕(2箇月)和年老(18箇月)小鼠分彆腹腔註射LPS,對照組註射PBS,24h後處死小鼠,分離腹腔巨噬細胞,收集細胞,real time PCR檢測IL-6的錶達水平.未經LPS刺激的小鼠腹腔巨噬細胞,體外用LPS處理24h,PBS作為對照,ELISA方法檢測細胞上清中的IL-6濃度.結果 自然狀態下衰老小鼠腹腔巨噬細胞中IL-6的錶達水平高于年輕小鼠(t=-18.538,P＜0.01).腹腔註射LPS後,IL-6在兩箇年齡組小鼠腹腔巨噬細胞中的錶達均顯著上升,但在衰老小鼠中的錶達明顯低于年輕小鼠(t =6.124,P＜0.01),差異有統計學意義.分離齣來的小鼠腹腔巨噬細胞,在沒有及有LPS刺激的情況下,衰老小鼠體外培養的細胞分泌炎癥因子IL-6的功能與年輕小鼠相比均顯著下降(t值分彆為12.329和5.876,P值均＜0.05),差異均有統計學意義.結論 衰老小鼠腹腔巨噬細胞在體內持續錶達高水平的IL-6,接受LPS刺激後,細胞誘導閤成IL-6的能力明顯衰退.腹腔巨噬細胞脫離體內環境後,衰老小鼠在體外錶現齣明顯的免疫功能衰退現象,分泌細胞因子IL-6的能力相對于年輕組明顯減弱.
목적 탐토지다당(LPS)대년경화노령소서복강거서세포체내외표체염성세포인자IL-6적영향.방법 년경(2개월)화년로(18개월)소서분별복강주사LPS,대조조주사PBS,24h후처사소서,분리복강거서세포,수집세포,real time PCR검측IL-6적표체수평.미경LPS자격적소서복강거서세포,체외용LPS처리24h,PBS작위대조,ELISA방법검측세포상청중적IL-6농도.결과 자연상태하쇠로소서복강거서세포중IL-6적표체수평고우년경소서(t=-18.538,P＜0.01).복강주사LPS후,IL-6재량개년령조소서복강거서세포중적표체균현저상승,단재쇠로소서중적표체명현저우년경소서(t =6.124,P＜0.01),차이유통계학의의.분리출래적소서복강거서세포,재몰유급유LPS자격적정황하,쇠로소서체외배양적세포분비염증인자IL-6적공능여년경소서상비균현저하강(t치분별위12.329화5.876,P치균＜0.05),차이균유통계학의의.결론 쇠로소서복강거서세포재체내지속표체고수평적IL-6,접수LPS자격후,세포유도합성IL-6적능력명현쇠퇴.복강거서세포탈리체내배경후,쇠로소서재체외표현출명현적면역공능쇠퇴현상,분비세포인자IL-6적능력상대우년경조명현감약.
Objective To investigate the effect of lipopolysaccharide (LPS) on inflammatory cytockine interleukine-6 (IL-6) expression in peritoneal macrohages from young and aged mice both in vitro and in vivo.Methods Two age groups (young group:2-month-old,old group:18-month-old) were divided into two groups,respectively:LPS treated groups were peritoneally injected with LPS and control groups was treated with PBS.Twenty-four hours later,mice were killed and then the peritoneal cavity was flushed for peritoneal macrophages to investigate the expression level of IL-6 by real-time PCR.Macrophages from control groups were cultured and treated with PBS (control) or LPS in vitro for another 24 hours,and then cell culture supematant was collected to analyze IL-6 concentration by ELISA.Results IL-6 expression in macrophages from natural aged mice (control) were significantly higher than young mice ( t =-18.538,P ＜0.01 ).After LPS treatment in vivo,the expression of IL-6 increased much in both of the two age groups,but it was much lower in aged group compared to young group (t =6.124,P ＜00.01 ).When cultured in vitro,the function of IL-6 secretion in macrophage from aged group decrease significantly compared to young group,no matter in control or LPS-treated status (t =12.329 and t =5.876,P ＜ 0.05 ).Conclusion Sustained and high level of IL-6 in macrophage was expressed in aged group.While after LPS stimulation,the ability of IL-6 production declined significantly.Once out of the intemal environment,macrophages of aged mice appeared lower ability of IL-6 secretion and immunity function disorder.
