CAJ | 학술논문

目的检测激活的T_H1类淋巴细胞标记分子"T淋巴细胞免疫球蛋白域及粘蛋白域蛋白-3(Tim-3)"在受者体内不同部位T淋巴细胞上表达的变化,探讨其与急性排斥反应的关系.方法建立小鼠同基因和异基因心脏移植模型(简称:同基因组和异基因组);移植术后第3和第6天,分离和制备两组受者外周血、脾脏、引流淋巴结和移植心内淋巴细胞悬液,采用流式细胞仪检测Tim-3阳性细胞在CD4~+ 和CD8~+ T淋细胞中的比值.结果两组受者术后外周血和脾脏内Tim-3~+/CD4~+以及Tim-3~+/CD8~+ 的比值比较,差异均无统计学意义(P>O.05).与同基因组比较,异基因组引流淋巴结内Tim-3~+/CD4~+ 比值轻度升高(P<0.05);但异基因组术后第6天与第3天比较,差异无统计学意义(P>0.05).与同基因组比较,移植心内Tim-3~+/CD4~+和TiM-3~+/CD8~+比值均显著升高(P<0.01);异基因组术后第6天与第3天比较.移植心TiM-3~+/CD4~+和Tim-3~+/CD8~+比值也显著升高(P<0.01).结论小鼠异基因心脏移植受者引流淋巴结和移植心内T淋巴细胞上Tim-3的表达升高与急性排斥反应的进展动态相关.
목적 검측격활적T_H1류림파세포표기분자"T림파세포면역구단백역급점단백역단백-3(Tim-3)"재수자체내불동부위T림파세포상표체적변화,탐토기여급성배척반응적관계.방법 건립소서동기인화이기인심장이식모형(간칭:동기인조화이기인조);이식술후제3화제6천,분리화제비량조수자외주혈、비장、인류림파결화이식심내림파세포현액,채용류식세포의검측Tim-3양성세포재CD4~+ 화CD8~+ T림세포중적비치.결과 량조수자술후외주혈화비장내Tim-3~+/CD4~+이급Tim-3~+/CD8~+ 적비치비교,차이균무통계학의의(P>O.05).여동기인조비교,이기인조인류림파결내Tim-3~+/CD4~+ 비치경도승고(P<0.05);단이기인조술후제6천여제3천비교,차이무통계학의의(P>0.05).여동기인조비교,이식심내Tim-3~+/CD4~+화TiM-3~+/CD8~+비치균현저승고(P<0.01);이기인조술후제6천여제3천비교.이식심TiM-3~+/CD4~+화Tim-3~+/CD8~+비치야현저승고(P<0.01).결론 소서이기인심장이식수자인류림파결화이식심내T림파세포상Tim-3적표체승고여급성배척반응적진전동태상관.
Objective To explore the expression level of Tim-3,the marker of activated T_H 1 cells.in T lymphocytes in different sites from recipients with acute rejection.Methods The model of cervical heterotopic heart transplantation was established in mice Two groups were get up:the isograft group(C57BL/6→C57BL/6) and the allograft group (Balb/c→C57BL/6).Lymphocytes were isolated from peripheral blood,spleens,draining lymph nodes and grafts 3 or 6 days after transplantation.The expression of TIM-3 in CD4~+ and CD8~+ T subsets was detected by flow cytometry.Results There was no significant difference in Tim-3~+/CD4~+ and Tim-3~+/CD8~+ ratio in peripheral blood or spleens between two groups.As compared with the isograft group,the proportion of Tirn-3~+/CD4~+ cells was slightly elevated in draining lymph node(P<0.05),but the percentage of Tim-3~+/CD4~+ cells had no significant change between 3 days and 6 days in the allograft group(P>0.05).The expression of Tim-3 in CD4~+ and CD8~+ of graft infiltrating T cells was obviously increased in allograft group(P<0.01),and it was significantly (P<0.01) up-regulated on the 6th day as compared with that on the 3rd day.Conclusion The dynamic changes of Tim-3 expression in T lymphocytes in draining lymph node and graft were correlated with the progresston oi acute rejection in mice.