CAJ | 학술논문

目的研究褪黑激素对体外培养的人宫颈癌(HeLa)细胞活性的影响.方法KTT测定褪黑激素的抗肿瘤活性;电镜观察药物对肿瘤细胞形态学的影响;流式细胞仪检测药物对肿瘤细胞周期的影响.结果高浓度(2 mmol@L-1)褪黑激素可抑制HeLa细胞的生长,使细胞倍增时间TD延长,细胞杀伤率达61.0%.用此浓度作用3天,可使S期细胞的比例减少,细胞形态学发生非特异性改变,有较多的坏死细胞和少量凋亡细胞.结论高浓度褪黑激素抑制HeLa细胞增殖,可能与抑制细胞的S期有关.
목적연구퇴흑격소대체외배양적인궁경암(HeLa)세포활성적영향.방법KTT측정퇴흑격소적항종류활성;전경관찰약물대종류세포형태학적영향;류식세포의검측약물대종류세포주기적영향.결과고농도(2 mmol@L-1)퇴흑격소가억제HeLa세포적생장,사세포배증시간TD연장,세포살상솔체61.0%.용차농도작용3천,가사S기세포적비례감소,세포형태학발생비특이성개변,유교다적배사세포화소량조망세포.결론고농도퇴흑격소억제HeLa세포증식,가능여억제세포적S기유관.
Aim To investigate the influences of melatonin (MT) on the growth of HeLa cells in vitro. Methods Theantiprolfferation activities of MT were evaluated in HeLa cells by means of trypan blue dye exclusion and MTT vital staining.The morphological changes of HeLa cells induced by MT were observed under transmission electronic microscope. Cell divisioncycle influenced by MT was assessed by a flow cytometry. Results MT produced a certain inhibition of HeLa cells at the con-centration of 2 mmol@ L-1 and prolonged the TD. The fraction of cells inhibited was 61.0%. The IC. so of HeLa cells exposed toMT for 96 h was 2.039 mmol@ L- 1. The flow cytometric analyses showed that exposure to MT for 72 h reduced the number ofHeLa cells in phase S. Under electronic microscope, the HeLa cells exposed to MT for 72 h displayed morphological changesof necrosis, apoptosis, more hetero-chromosome and less somatic chromosome. Conclusion MT showed certain influences onthe growth of HeLa cells. Its mechanism may probably be attributable to reduction of the number of cells in phase S.