作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2000年
5期
594-598
,共5页
甘薯%贮藏蛋白基因%启动子%信号肽编码区%PCR%序列分析
甘藷%貯藏蛋白基因%啟動子%信號肽編碼區%PCR%序列分析
감서%저장단백기인%계동자%신호태편마구%PCR%서렬분석
Ipomoea batatas%Sporamin gene%Promoter%Signal peptide-coding region%PCR%Sequence analysis
根据已知的甘薯块根贮藏蛋白A基因序列，设计和合成了两对PCR引物，从南薯88基因组中分别扩增出该基因的启动子和启动子加信号肽编码区两种DNA片段，并测定了它们的DNA序列。将测定的序列与GenBank中的甘薯贮藏蛋白基因的相应序列进行同源性分析，结果发现，其中既有与GenBank中甘薯贮藏蛋白基因启动子完全相同的片段，也有发生了较大变异的片段，这些变异涉及到调控表达的顺式作用元件。在信号肽编码区，虽存在碱基序列变异，但比启动子区更保守，特别是原肽编码区未发现碱基变异。
根據已知的甘藷塊根貯藏蛋白A基因序列，設計和閤成瞭兩對PCR引物，從南藷88基因組中分彆擴增齣該基因的啟動子和啟動子加信號肽編碼區兩種DNA片段，併測定瞭它們的DNA序列。將測定的序列與GenBank中的甘藷貯藏蛋白基因的相應序列進行同源性分析，結果髮現，其中既有與GenBank中甘藷貯藏蛋白基因啟動子完全相同的片段，也有髮生瞭較大變異的片段，這些變異涉及到調控錶達的順式作用元件。在信號肽編碼區，雖存在堿基序列變異，但比啟動子區更保守，特彆是原肽編碼區未髮現堿基變異。
근거이지적감서괴근저장단백A기인서렬，설계화합성료량대PCR인물，종남서88기인조중분별확증출해기인적계동자화계동자가신호태편마구량충DNA편단，병측정료타문적DNA서렬。장측정적서렬여GenBank중적감서저장단백기인적상응서렬진행동원성분석，결과발현，기중기유여GenBank중감서저장단백기인계동자완전상동적편단，야유발생료교대변이적편단，저사변이섭급도조공표체적순식작용원건。재신호태편마구，수존재감기서렬변이，단비계동자구경보수，특별시원태편마구미발현감기변이。
Two DNA fragments,promoter(fragment 1)and promoter with signal peptidecoding sequence (fragment 2) of sporamin from sweet potato Nahshu 88,a widely cultivated variety in China,were amplified by the polymerase chain reaction (PCR).The amplified fragments were cloned and then sequenced. Sequence comparison showed that the fragment 1 sequence was almost identical to the corresponding region of sporamin gene, gSPO-A1, but different from that of gSPOR5-31.The sequence of fragment 2,including some cis regulatory elements,was different from the corresponding region of gSPO-A1 and gSPOR5-31.Comparison of signal peptide-coding sequence among fragment 2,gSPO-A1 and gSPOR5-31 showed 8 nucleotides changed,which appeared in the region of pre-segment, not in the region of pro-segment.These results indicated that the 5’-fianking region of sporamin genes was much conserved, but there was some variation,especially in the promoter region,which maybe play some role in gene expression.
