水产科学
水產科學
수산과학
FISHERIES SCIENCE
2009年
12期
783-785
,共3页
王丽梅%许伟定%王笑月%韩家波
王麗梅%許偉定%王笑月%韓傢波
왕려매%허위정%왕소월%한가파
光棘球海胆%中间球海胆%人工培育%杂交海胆
光棘毬海膽%中間毬海膽%人工培育%雜交海膽
광극구해담%중간구해담%인공배육%잡교해담
Strongylocentrotus nudus%S.intermedius%hybridization
以光棘球海胆为母本,中间球海胆为父本进行杂交试验及苗种培育.试验结果表明,通过筛选同时得到自然成熟的父母本精、卵,在比正常海胆自交精子量高40倍以上的精子作用下,杂交受精率为2.5%.在适宜的水温、光照及饵料等的培养条件下,杂交海胆能够生长附着变态,变态时间与母本相近.培育出的杂交海胆外部颜色及棘色、棘长介于两亲本之间.杂交海胆经过6~7个月的中间暂养,出苗量为0.6×10~4个/m~3,个体壳径3~30 mm,平均壳径8.8 mm.
以光棘毬海膽為母本,中間毬海膽為父本進行雜交試驗及苗種培育.試驗結果錶明,通過篩選同時得到自然成熟的父母本精、卵,在比正常海膽自交精子量高40倍以上的精子作用下,雜交受精率為2.5%.在適宜的水溫、光照及餌料等的培養條件下,雜交海膽能夠生長附著變態,變態時間與母本相近.培育齣的雜交海膽外部顏色及棘色、棘長介于兩親本之間.雜交海膽經過6~7箇月的中間暫養,齣苗量為0.6×10~4箇/m~3,箇體殼徑3~30 mm,平均殼徑8.8 mm.
이광극구해담위모본,중간구해담위부본진행잡교시험급묘충배육.시험결과표명,통과사선동시득도자연성숙적부모본정、란,재비정상해담자교정자량고40배이상적정자작용하,잡교수정솔위2.5%.재괄의적수온、광조급이료등적배양조건하,잡교해담능구생장부착변태,변태시간여모본상근.배육출적잡교해담외부안색급극색、극장개우량친본지간.잡교해담경과6~7개월적중간잠양,출묘량위0.6×10~4개/m~3,개체각경3~30 mm,평균각경8.8 mm.
Two species sea urchins, Strongylocentrotus nudus(♀) and S. Internidius(♂), were hybridized with the average fertilization success of about 2.5%. When the eggs of S. Nudus were fertilized with sperm of S. Intermedius, the amount of sperm selected synchronously was used at a rate of above 40 times than that in normal fertilization. Besides, the hybrid larvae had normal growth and metamorphosis at suitable water temperature, light and feeding regimes, and the period of whole metamorphosis were almost the same as their broodstocks. The hybrids had intermediate body color and thorns between their broodstocks. Total of 0.6×10~4 individuals/m~3 was obtained with test diameter from 2.59 to 2.88 mm,average of 8.8 mm during 6~7 month rearing.