解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2010年
1期
27-31
,共5页
李双成%康云霄%石葛明%崔慧先
李雙成%康雲霄%石葛明%崔慧先
리쌍성%강운소%석갈명%최혜선
乙醇%酪氨酸羟化酶%多巴胺转运体%免疫组织化学%流式细胞术%免疫印迹法%大鼠
乙醇%酪氨痠羥化酶%多巴胺轉運體%免疫組織化學%流式細胞術%免疫印跡法%大鼠
을순%락안산간화매%다파알전운체%면역조직화학%류식세포술%면역인적법%대서
Ethanol%Tyrosine hydroxylase%Dopamine transporter%Immunohistochemistry%Flow cytometry%Western blotting%Rat
目的 观察乙醇处理大鼠脑内酪氨酸羟化酶(TH)和多巴胺转运体(DAT)的表达变化,判断乙醇对脑内多巴胺(DA)能体系的影响.方法 选取Wistar大鼠60只,随机分为对照组和乙醇处理组,每组30只,乙醇处理组大鼠以20%乙醇代替饮水饲养6个月;利用免疫组织化学、流式细胞术及免疫印迹等方法,分析乙醇处理大鼠有关脑区TH和DAT的表达改变.结果 1.免疫细胞化学法研究发现,乙醇处理组大鼠脑内黑质(SN)-腹侧被盖区(VTA)、尾壳核(CPu) 和伏核(NACC)TH的灰度值明显低于对照组(P<0.05);尾壳核和伏核DAT的灰度值明显低于对照组(P<0.05).2.流式细胞术检测发现,乙醇处理组大鼠中脑TH的表达量明显高于对照组(P<0.05).3.免疫印迹检测发现,乙醇处理组大鼠所观察脑区TH和DAT与β-actin条带相对吸光度比值明显高于对照组(P<0.05).结论 乙醇处理增加大鼠脑内TH和DAT的表达.
目的 觀察乙醇處理大鼠腦內酪氨痠羥化酶(TH)和多巴胺轉運體(DAT)的錶達變化,判斷乙醇對腦內多巴胺(DA)能體繫的影響.方法 選取Wistar大鼠60隻,隨機分為對照組和乙醇處理組,每組30隻,乙醇處理組大鼠以20%乙醇代替飲水飼養6箇月;利用免疫組織化學、流式細胞術及免疫印跡等方法,分析乙醇處理大鼠有關腦區TH和DAT的錶達改變.結果 1.免疫細胞化學法研究髮現,乙醇處理組大鼠腦內黑質(SN)-腹側被蓋區(VTA)、尾殼覈(CPu) 和伏覈(NACC)TH的灰度值明顯低于對照組(P<0.05);尾殼覈和伏覈DAT的灰度值明顯低于對照組(P<0.05).2.流式細胞術檢測髮現,乙醇處理組大鼠中腦TH的錶達量明顯高于對照組(P<0.05).3.免疫印跡檢測髮現,乙醇處理組大鼠所觀察腦區TH和DAT與β-actin條帶相對吸光度比值明顯高于對照組(P<0.05).結論 乙醇處理增加大鼠腦內TH和DAT的錶達.
목적 관찰을순처리대서뇌내락안산간화매(TH)화다파알전운체(DAT)적표체변화,판단을순대뇌내다파알(DA)능체계적영향.방법 선취Wistar대서60지,수궤분위대조조화을순처리조,매조30지,을순처리조대서이20%을순대체음수사양6개월;이용면역조직화학、류식세포술급면역인적등방법,분석을순처리대서유관뇌구TH화DAT적표체개변.결과 1.면역세포화학법연구발현,을순처리조대서뇌내흑질(SN)-복측피개구(VTA)、미각핵(CPu) 화복핵(NACC)TH적회도치명현저우대조조(P<0.05);미각핵화복핵DAT적회도치명현저우대조조(P<0.05).2.류식세포술검측발현,을순처리조대서중뇌TH적표체량명현고우대조조(P<0.05).3.면역인적검측발현,을순처리조대서소관찰뇌구TH화DAT여β-actin조대상대흡광도비치명현고우대조조(P<0.05).결론 을순처리증가대서뇌내TH화DAT적표체.
Objective To study the effect of ethonal on the dopaminergic system by analyzing the altered expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the brain of ethanol-treated rats. Methods Sixty Wistar rats were selected and divided into control group and ethanol-treated group, 30 per group, the ethanol-treated rats were treated with 20% ethanol for 6 months. Immunohistochemistry, flow cytometry and Western blotting were used to analyze the altered expression of TH and DAT in the DA energic system in different brain regions of the ethanol treated rats. Results 1. Immunohistochemistry showed the mean gray value of TH in substantia nigra(SN)-ventrotegmental area (VTA), caudae putamen (Cpu) and nucleus accumbens (NACC), DAT in Cpu and NACC of the ethanol were smaller than those in control (P<0.05). 2. Flow cytometry showed the expression of TH in middle brain of the ethanol-treated rats increased significantly compared with the control(P<0.05). 3. Western blotting showed the ratio of IA of TH/β-actin and DAT/β-actin in different brain regions of the ethanol-treated rats were larger than those in control(P<0.05).Conclusion Ethanol treatment increases the expression of TH and DAT in rat brain.
