中国药理学与毒理学杂志
中國藥理學與毒理學雜誌
중국약이학여독이학잡지
CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY
2010年
1期
30-34
,共5页
李中港%秦慧迪%汪怀山%史艳秋%刘兆平
李中港%秦慧迪%汪懷山%史豔鞦%劉兆平
리중항%진혜적%왕부산%사염추%류조평
超敏反应%速发型%模型%动物%免疫球蛋白E%组胺%类胰蛋白酶类
超敏反應%速髮型%模型%動物%免疫毬蛋白E%組胺%類胰蛋白酶類
초민반응%속발형%모형%동물%면역구단백E%조알%류이단백매류
hypersensitivity,immediate%models,animal%immunoglobulin E%histamine%tryptases
目的 比较BN大鼠和Wistar大鼠在Ⅰ型超敏反应中的敏感性,建立一种灵敏可靠的Ⅰ型超敏反应检测体系.方法 BN大鼠和Wistar大鼠分别隔天sc不同剂量的卵白蛋白(10,20和40 μg·kg~(-1)),共5次,正常对照组sc给予生理盐水.首次注射后第21 天取血清, 用ELISA法测定血清总免疫球蛋白E(IgE)水平,通过被动皮肤过敏反应实验检测其特异性IgE水平;第22天检测激发后大鼠血压、血清中组胺和类胰蛋白酶浓度的变化.结果 与正常对照组比较,BN大鼠在卵白蛋白10,20和40 μg·kg~(-1)下血清总IgE和特异性IgE显著增加,血压下降,血清组胺和类胰蛋白酶浓度增加;Wistar大鼠仅在卵白蛋白40 μg·kg~(-1)组出现上述变化.结论 与Wistar大鼠相比,BN大鼠用于Ⅰ型超敏反应的检测更为灵敏.血压和血清总IgE、特异性IgE、组胺及类胰蛋白酶浓度等可作为Ⅰ型超敏反应重要的检测指标.
目的 比較BN大鼠和Wistar大鼠在Ⅰ型超敏反應中的敏感性,建立一種靈敏可靠的Ⅰ型超敏反應檢測體繫.方法 BN大鼠和Wistar大鼠分彆隔天sc不同劑量的卵白蛋白(10,20和40 μg·kg~(-1)),共5次,正常對照組sc給予生理鹽水.首次註射後第21 天取血清, 用ELISA法測定血清總免疫毬蛋白E(IgE)水平,通過被動皮膚過敏反應實驗檢測其特異性IgE水平;第22天檢測激髮後大鼠血壓、血清中組胺和類胰蛋白酶濃度的變化.結果 與正常對照組比較,BN大鼠在卵白蛋白10,20和40 μg·kg~(-1)下血清總IgE和特異性IgE顯著增加,血壓下降,血清組胺和類胰蛋白酶濃度增加;Wistar大鼠僅在卵白蛋白40 μg·kg~(-1)組齣現上述變化.結論 與Wistar大鼠相比,BN大鼠用于Ⅰ型超敏反應的檢測更為靈敏.血壓和血清總IgE、特異性IgE、組胺及類胰蛋白酶濃度等可作為Ⅰ型超敏反應重要的檢測指標.
목적 비교BN대서화Wistar대서재Ⅰ형초민반응중적민감성,건립일충령민가고적Ⅰ형초민반응검측체계.방법 BN대서화Wistar대서분별격천sc불동제량적란백단백(10,20화40 μg·kg~(-1)),공5차,정상대조조sc급여생리염수.수차주사후제21 천취혈청, 용ELISA법측정혈청총면역구단백E(IgE)수평,통과피동피부과민반응실험검측기특이성IgE수평;제22천검측격발후대서혈압、혈청중조알화류이단백매농도적변화.결과 여정상대조조비교,BN대서재란백단백10,20화40 μg·kg~(-1)하혈청총IgE화특이성IgE현저증가,혈압하강,혈청조알화류이단백매농도증가;Wistar대서부재란백단백40 μg·kg~(-1)조출현상술변화.결론 여Wistar대서상비,BN대서용우Ⅰ형초민반응적검측경위령민.혈압화혈청총IgE、특이성IgE、조알급류이단백매농도등가작위Ⅰ형초민반응중요적검측지표.
OBJECTIVE To compare sensitivity in typeⅠ hypersensitivity between BN and Wistar rats, and to establish a sensitive and reliable determination system for typeⅠ hypersensitivity. METHODS BN and Wistar rats were sc given ovalbumin (OVA) 10, 20 and 40 μg·kg~(-1) every other day for 5 times and normal control group with sc normal saline. The total immunoglobulin E (IgE) levels in serum were determined with ELISA and the specific IgE levels in serum were determined by passive cutaneous anaphylaxis on the 21st day from the 1st injection. The blood pressure, serum histamine and tryptase levels were determined after challenge on the 22nd day. RESULTSTotal IgE, specific IgE, histamine and tryptase levels in serum significantly increased and blood pressure decreased in OVA 10, 20 and 40 μg·kg~(-1) BN rat groups compared with normal control group, while in Wistar rats these symptoms only appeared in OVA 40 μg·kg~(-1) group. CONCLUSION BN rats are more sensitive than Wistar rats in typeⅠ hypersensitivity. The blood pressure, serum total IgE, specific IgE, histamine and tryptase levels can be used as the important indicators in typeⅠ hypersensitivity.
