CAJ | 학술논문

利用光谱法研究了β-环糊精与二代抗凝杀鼠剂溴鼠灵的超分子作用,根据Benesi-Hildebrand法确定二者的包络比为1:1,室温下包络常数为1.048×10~(-4)L·mol~(-1).实验还考察了二者的包络机理,推测主要是溴鼠灵结构中的疏水基团联苯基进入了β-环糊精的疏水空腔.同时发现,超分子包络物的形成可以大大增强溴鼠灵的荧光发射,据此,建立了水溶液中测定溴鼠灵的荧光分析方法.在优化实验条件下,线性范围8.0×10~(-8)～4.0×10~(-6)mol·L~(-1)(r=0.999 4),检出限为8.8×10~(-9)mol·L~(-1),并成功地用于环境水中微量溴鼠灵的测定.
이용광보법연구료β-배호정여이대항응살서제추서령적초분자작용,근거Benesi-Hildebrand법학정이자적포락비위1:1,실온하포락상수위1.048×10~(-4)L·mol~(-1).실험환고찰료이자적포락궤리,추측주요시추서령결구중적소수기단련분기진입료β-배호정적소수공강.동시발현,초분자포락물적형성가이대대증강추서령적형광발사,거차,건립료수용액중측정추서령적형광분석방법.재우화실험조건하,선성범위8.0×10~(-8)～4.0×10~(-6)mol·L~(-1)(r=0.999 4),검출한위8.8×10~(-9)mol·L~(-1),병성공지용우배경수중미량추서령적측정.
The supramolecular interaction between β-cyclodextrin and brodifacoum, an anticoagulant rodenticide of the second generation, was studied by spectroscopy. The results showed that brodifacoum and β-cyclodextrin could form an inclusion com-plex with an association constant of 1.048×10~4 L·mol~(-1) and a 1 : 1 stoichiometry based on Benesi-Hildebrand equation. The inclusion mechanism was proposed to explain the inclusion mode. It was indicated that the hydrophohie group of brodifacoum molecule, biphenyl, entered into the cavity of β-cyclodextrin. At the same time, it was also observed the significant enhancement of fluorescence of brodifacoum after forming inclusion complex. According to the fluorescence enhancement phenomenon, a spec-trofluorimetric method of detecting brodifacoum in aqueous media was established with the linear range of 8.0×10~(-8)-4.0×10~(-6) mol·L~(-1) and the correlation coefficient of 0. 999 4. The detection limit of the method was 8.8×10~(-9) mol · L~(-1). The proposed method was successfully applied to determine the trace amount of brodifacoum in environment water and the recovery was in the range of 87.3% to 103.9%.