黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2011年
8期
80-82
,共3页
孙延芳%梁宗锁%杨开宝%刘政
孫延芳%樑宗鎖%楊開寶%劉政
손연방%량종쇄%양개보%류정
酸枣%有机酸%高效液相色谱
痠棘%有機痠%高效液相色譜
산조%유궤산%고효액상색보
sour jujube%organic acids%high performance liquid chromatography(HPLC)
为更好地开发利用酸枣资源,采用Waters XB-C18色谱柱(250 mm×4.6 mm,5μm),以0.05 mol.L-1磷酸氢二钠溶液为流动相,流速1.0 mL.min-1,紫外检测器检测波长210 nm,建立高效液相色谱(HPLC),对酸枣(干果、鲜果及其果酱)中有机酸和维生素C进行测定分析。结果表明:色谱图在10 min内可很好地实现7种有机酸和维生素C的基线分离,并且酸枣(干果、鲜果和果酱)中草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、琥珀酸和维生素C含量差别很大。该方法具有分析速度快、线性范围宽、灵敏度高、重复性好等优点,适用于酸枣有机酸和维生素C的检测。
為更好地開髮利用痠棘資源,採用Waters XB-C18色譜柱(250 mm×4.6 mm,5μm),以0.05 mol.L-1燐痠氫二鈉溶液為流動相,流速1.0 mL.min-1,紫外檢測器檢測波長210 nm,建立高效液相色譜(HPLC),對痠棘(榦果、鮮果及其果醬)中有機痠和維生素C進行測定分析。結果錶明:色譜圖在10 min內可很好地實現7種有機痠和維生素C的基線分離,併且痠棘(榦果、鮮果和果醬)中草痠、酒石痠、蘋果痠、乳痠、乙痠、檸檬痠、琥珀痠和維生素C含量差彆很大。該方法具有分析速度快、線性範圍寬、靈敏度高、重複性好等優點,適用于痠棘有機痠和維生素C的檢測。
위경호지개발이용산조자원,채용Waters XB-C18색보주(250 mm×4.6 mm,5μm),이0.05 mol.L-1린산경이납용액위류동상,류속1.0 mL.min-1,자외검측기검측파장210 nm,건립고효액상색보(HPLC),대산조(간과、선과급기과장)중유궤산화유생소C진행측정분석。결과표명:색보도재10 min내가흔호지실현7충유궤산화유생소C적기선분리,병차산조(간과、선과화과장)중초산、주석산、평과산、유산、을산、저몽산、호박산화유생소C함량차별흔대。해방법구유분석속도쾌、선성범위관、령민도고、중복성호등우점,괄용우산조유궤산화유생소C적검측。
In order to better develop and utilize sour jujube resources,an Waters XB-C18 column was used and 0.05 mol·L-1 phosphate buffer solution as the mobile phase at a flow rate of 1.0 mL·min-1,and the detection wavelenghth was 210 nm.The organic acids and Vc by HPLC in sour jujube(fresh fruit,dry fruit and jam) were determined and analyzed.The result showed that organic acids and Vc were successfully separated within 10 min,but the content of organic acids and Vc in sour jujube(fresh fruit,dry fruit and jam)were very different.The method was fast,sensitive,reproducible and practical for the routine analysis of organic acids and Vc in sour jujube.