国际放射医学核医学杂志
國際放射醫學覈醫學雜誌
국제방사의학핵의학잡지
INTERNATIONAL JOURNAL OF RADIATION MEDICINE AND NUCLEAR MEDICINE
2011年
4期
217-219,248
,共4页
杨爱民%于燕%邓惠兴%李杰
楊愛民%于燕%鄧惠興%李傑
양애민%우연%산혜흥%리걸
放射疗法%氟脱氧葡萄糖F18%体层摄影术,发射型计算机,单光子%HL-91
放射療法%氟脫氧葡萄糖F18%體層攝影術,髮射型計算機,單光子%HL-91
방사요법%불탈양포도당F18%체층섭영술,발사형계산궤,단광자%HL-91
Radiotherapy%Fluorodeoxyglucose F18%Tomography,emission-computed,single-photon%HL91
目的 用99Tcm-4,9-二氮-2,3,10,10-四甲基十二烷-2,11-二酮肟(99Tcm-HL91)和18F-FDG为显像剂,探讨肿瘤放疗前、后的乏氧状态与放疗疗效之间的关系.方法 ①动物:选取20只昆明种、清洁级健康成年雄性小鼠供实验用(另备5只用于腹水模型),采用完全随机方法分为2组(对照组和实验组),每组10只.②细胞:将S180腹水瘤细胞株复苏后分别接种于5只小鼠腹腔,待腹水形成后,抽取1 ml稀释至活瘤细胞计数大于2×106个/ml瘤细胞悬液,于每只小鼠右后肢皮下接种0.2 ml.待瘤体长至1～1.5 cm时用于实验.③99Tcm-HL91显像:通过小鼠尾静脉注射99Tcm-HL91 37 MBq,4h后分别于放疗前、放疗后1h、2d和10d进行最像.④18F-FDG显像:分别于放疗前和放疗后11d进行18F-FDG显像.显像前12 h禁食,小鼠尾静脉注射18F-FDG 11.5MBq,30 min后腹腔注射0.1％戊巴比妥钠麻醉.⑤放射治疗:第1次99Tcm-HL91和18F-FDG显像结束后,2组小鼠分别给予0 Gy和8 Gy的X线照射.⑥图像处理和半定量分析:于图像上肿瘤部位和小鼠肺野设等大感兴趣区(ROI),获得显像中瘤/非瘤(T/NT)摄取比值(UR).结果 ①放疗前和放疗后1h、2d、10d 99Tcm-HL91的UR分别为3.53±1.62、3.41±1.42、2.55±1.57和1.26±0.03,表明放疗后肿瘤出现再氧合,乏氧状态依次减低.对照组各时相的UR值分别为3.62±1.65、3.02±1.94、4.10±1.48和2.96±2.02,UR无明显递减趋势,表明肿瘤乏氧状态未见减低,甚至在2d时段出现加重.②放疗前和放疗后11 d 18F-FDG显像的UR分别为2.49±1.29和1.49±0.56,UR亦呈现依次递减趋势,与99Tcm-HL91显像一致.表明放疗后肿瘤葡萄糖代谢活性减低.对照组的UR分别为2.22±0.45和1.89±0.08,UR也出现减低,但变化不明显,而且大部分小鼠在显像前死亡.结论 放疗前后99cm-HL91显像的UR能够较好地评价肿瘤乏氧水平,并可初步评价疗效,为在活体评价放疗后肿瘤再氧合过程提供了一种新方法.
目的 用99Tcm-4,9-二氮-2,3,10,10-四甲基十二烷-2,11-二酮肟(99Tcm-HL91)和18F-FDG為顯像劑,探討腫瘤放療前、後的乏氧狀態與放療療效之間的關繫.方法 ①動物:選取20隻昆明種、清潔級健康成年雄性小鼠供實驗用(另備5隻用于腹水模型),採用完全隨機方法分為2組(對照組和實驗組),每組10隻.②細胞:將S180腹水瘤細胞株複囌後分彆接種于5隻小鼠腹腔,待腹水形成後,抽取1 ml稀釋至活瘤細胞計數大于2×106箇/ml瘤細胞懸液,于每隻小鼠右後肢皮下接種0.2 ml.待瘤體長至1～1.5 cm時用于實驗.③99Tcm-HL91顯像:通過小鼠尾靜脈註射99Tcm-HL91 37 MBq,4h後分彆于放療前、放療後1h、2d和10d進行最像.④18F-FDG顯像:分彆于放療前和放療後11d進行18F-FDG顯像.顯像前12 h禁食,小鼠尾靜脈註射18F-FDG 11.5MBq,30 min後腹腔註射0.1％戊巴比妥鈉痳醉.⑤放射治療:第1次99Tcm-HL91和18F-FDG顯像結束後,2組小鼠分彆給予0 Gy和8 Gy的X線照射.⑥圖像處理和半定量分析:于圖像上腫瘤部位和小鼠肺野設等大感興趣區(ROI),穫得顯像中瘤/非瘤(T/NT)攝取比值(UR).結果 ①放療前和放療後1h、2d、10d 99Tcm-HL91的UR分彆為3.53±1.62、3.41±1.42、2.55±1.57和1.26±0.03,錶明放療後腫瘤齣現再氧閤,乏氧狀態依次減低.對照組各時相的UR值分彆為3.62±1.65、3.02±1.94、4.10±1.48和2.96±2.02,UR無明顯遞減趨勢,錶明腫瘤乏氧狀態未見減低,甚至在2d時段齣現加重.②放療前和放療後11 d 18F-FDG顯像的UR分彆為2.49±1.29和1.49±0.56,UR亦呈現依次遞減趨勢,與99Tcm-HL91顯像一緻.錶明放療後腫瘤葡萄糖代謝活性減低.對照組的UR分彆為2.22±0.45和1.89±0.08,UR也齣現減低,但變化不明顯,而且大部分小鼠在顯像前死亡.結論 放療前後99cm-HL91顯像的UR能夠較好地評價腫瘤乏氧水平,併可初步評價療效,為在活體評價放療後腫瘤再氧閤過程提供瞭一種新方法.
목적 용99Tcm-4,9-이담-2,3,10,10-사갑기십이완-2,11-이동우(99Tcm-HL91)화18F-FDG위현상제,탐토종류방료전、후적핍양상태여방료료효지간적관계.방법 ①동물:선취20지곤명충、청길급건강성년웅성소서공실험용(령비5지용우복수모형),채용완전수궤방법분위2조(대조조화실험조),매조10지.②세포:장S180복수류세포주복소후분별접충우5지소서복강,대복수형성후,추취1 ml희석지활류세포계수대우2×106개/ml류세포현액,우매지소서우후지피하접충0.2 ml.대류체장지1～1.5 cm시용우실험.③99Tcm-HL91현상:통과소서미정맥주사99Tcm-HL91 37 MBq,4h후분별우방료전、방료후1h、2d화10d진행최상.④18F-FDG현상:분별우방료전화방료후11d진행18F-FDG현상.현상전12 h금식,소서미정맥주사18F-FDG 11.5MBq,30 min후복강주사0.1％무파비타납마취.⑤방사치료:제1차99Tcm-HL91화18F-FDG현상결속후,2조소서분별급여0 Gy화8 Gy적X선조사.⑥도상처리화반정량분석:우도상상종류부위화소서폐야설등대감흥취구(ROI),획득현상중류/비류(T/NT)섭취비치(UR).결과 ①방료전화방료후1h、2d、10d 99Tcm-HL91적UR분별위3.53±1.62、3.41±1.42、2.55±1.57화1.26±0.03,표명방료후종류출현재양합,핍양상태의차감저.대조조각시상적UR치분별위3.62±1.65、3.02±1.94、4.10±1.48화2.96±2.02,UR무명현체감추세,표명종류핍양상태미견감저,심지재2d시단출현가중.②방료전화방료후11 d 18F-FDG현상적UR분별위2.49±1.29화1.49±0.56,UR역정현의차체감추세,여99Tcm-HL91현상일치.표명방료후종류포도당대사활성감저.대조조적UR분별위2.22±0.45화1.89±0.08,UR야출현감저,단변화불명현,이차대부분소서재현상전사망.결론 방료전후99cm-HL91현상적UR능구교호지평개종류핍양수평,병가초보평개료효,위재활체평개방료후종류재양합과정제공료일충신방법.
Objective To investigate the relationship between radiotherapy effect and 99Tcm-4,9-diaza-2,3,10,10-tetramethyldodecan-2,11-dioxime (99Tcm-HL91) and 18F -FDG imaging in S180 mouse.Methods ① Animals:twenty male Kunming mice were randomly divided into two groups of radiotherapy and non-radiotherapy control group.②Cells:S180 cell lines were injected into peritoneal cavity of the other 5 mice.When the S180 tumor developed,1ml liquid were dripped and diluted to the suspension solution of2×106cells.Then,0.2 ml cells solution were was injected into the hippo of right rear leg of mice.The mouse model was used to experiment while the tumor dimension developed to 1-1.5 cm.③99Tcm-HL91 imaging:37 MBq 99Tcm-HL91 was injected into mouse models by tail vein.After 4 h,SPECT were performed before and at 1 h,2 d and 10 d after radiotherapy.④18F-FDG imaging:11.5 MBq 18F-FDG was injected into mouse models by tail vein.After 30 min,SPECT were perfored before and after radiotherapy at the time of 11 d.⑤Radiotherapy:two groups of mice were irradiated to 0 Gy and 8 Gy X-ray after the first 99Tcm-HL91 and 18F-FDG imaging.⑥Images analysis:the region of interest(ROI) region,in tumor and lung site,was drawed to calculate the uptake ratio(UR).Results Before and at 1 h,2 d,and 10 d after radiotherapy,the UR in 99Tcm-HL91 imaging were 3.53±1.62,3.41± 1.42,2.55± 1.57 and 1.26±0.03,respectively,while the UR were 3.62±1.65,3.02±1.94,4.10±1.48 and 2.96±2.02 in control group.This revealed that tumors hypoxic level was decreased after radiation and suggested that tumors developed reoxygenation.Before and at 11d after radiotherapy,the UR in 18F-FDG imaging were 2.49±1.29 and 1.49±0.56,while the UR were 2.22±0.45 and 1.89±0.08 in control group,which suggested a coincident trend with 99Tcm-HL91 imaging.The tumor glucose metabolism rate was also decreased which suggested a good radiotherapy outcome.Conclusions 99Tcm-HL91 UR can effectively be used to evaluate the tumor hypoxic level in vivo.This affords a new useful method to assess the whole volume of tumor hypoxic level in clinic.
