CAJ | 학술논문

目的探讨携尿激酶靶向微泡溶解兔动脉内血栓后下的形态学改变。方法在24只新西兰大白兔单侧股动脉制作富含血小板的混合性血栓，分成4组，每组6只:①单纯超声照射组(US);②单纯尿激酶静脉注射组(UK);③超声照射+微泡造影剂+尿激酶静脉注射组(US+ M+ UK);④超声照射+RGDS微泡造影剂+尿激酶组(US+ R+ UK)。将RGDS、微泡造影剂(SonoVue)和尿激酶通过直接联合法配制成携尿激酶的靶向微泡，超声照射30 min，多普勒血流仪监测血流量变化评价溶栓效果，然后对其行HE染色、电镜检查。结果 US+ R+ UK组血流量完全恢复，实现完全再通（P＜0.001），HE染色显示血栓完全溶解，扫描电镜检查示血栓的纤维网状结构破坏，透射电镜显示血栓的大量降解碎片。结论血栓纤维蛋白网状结构的破坏、纤维蛋白的溶解是携尿激酶靶向微泡在兔动脉内溶解血栓的主要电镜表现。
목적 탐토휴뇨격매파향미포용해토동맥내혈전후하적형태학개변。방법 재24지신서란대백토단측고동맥제작부함혈소판적혼합성혈전，분성4조，매조6지:①단순초성조사조(US);②단순뇨격매정맥주사조(UK);③초성조사+미포조영제+뇨격매정맥주사조(US+ M+ UK);④초성조사+RGDS미포조영제+뇨격매조(US+ R+ UK)。장RGDS、미포조영제(SonoVue)화뇨격매통과직접연합법배제성휴뇨격매적파향미포，초성조사30 min，다보륵혈류의감측혈류량변화평개용전효과，연후대기행HE염색、전경검사。결과 US+ R+ UK조혈류량완전회복，실현완전재통（P＜0.001），HE염색현시혈전완전용해，소묘전경검사시혈전적섬유망상결구파배，투사전경현시혈전적대량강해쇄편。결론혈전섬유단백망상결구적파배、섬유단백적용해시휴뇨격매파향미포재토동맥내용해혈전적주요전경표현。
Objective To study the change of thrombosis in rabbits arteries by electron microscope after thromb was lyses by urokinase targeted microbubbles.Methods A total of 24 rabbits with plateletrich thrombi in the femoral artery were randomized into 4 treatment groups (n = 6) :(1) ultrasound alone (US) ; (2) urokinase alone (UK); (3) ultrasound, non-targeted microbubble and urokinase (US + M + UK) ;(4) ultrasound, platelet-targeted microbubble and urokinase (US + R + UK).Urokinase targeted microbubbles were in conjunction with the surface of commercial microbubbles (SonoVue) by direct conjugation method.US was simultaneously applied transcutaneously over the thrombus up to 30 min.The thrombolytic effect was based on ultrasound, blood flow and histological observations at 120 min post treatment.Results US + R + UK group was completed recanalization (P ＜ 0.001).Scanning electron microscope examination showed thrombosis of the fiber network structure damage.Conclusions The main electron microscope change of RGDS urokinase targeted microbubbles dissolve thromb is thrombus fibrin network structure damage and fibrin dissolution.