中国医药
中國醫藥
중국의약
CHINA MEDICINE
2011年
5期
541-543
,共3页
赵伟%宋旭臣%姜丽娟%张宏
趙偉%宋旭臣%薑麗娟%張宏
조위%송욱신%강려연%장굉
糖尿病%肺%肌动蛋白类%罗格列酮
糖尿病%肺%肌動蛋白類%囉格列酮
당뇨병%폐%기동단백류%라격렬동
Diabetes mellitus%Pulmonary%Actins%Rosiglitazone
目的 探讨自发性2型糖尿病(OLETF)大鼠肺组织α-平滑肌肌动蛋白(α-SMA)表达的变化及罗格列酮对其影响.方法 30周龄雄性OLETF大鼠16只,分为OLETF组和OLETF/L组每组8只,对照组为8只LETO组大鼠.OLETF/L组大鼠给予罗格列酮3 mg/(kg·d)灌胃12周后处死大白鼠,应用免疫组织化学和免疫蛋白印迹法检测各组大鼠肺组织α-SMA蛋白表达.结果 各组大鼠肺组织α-SMA主要表达于肌成纤维细胞、血管平滑肌细胞、小气道平滑肌,OLETF组大鼠肺组织α-SMA蛋白表达(2.03±0.64)明显高于LETO组(1.18±0.35),P<0.05];OLETF/L组明显减少(1.34±0.40),差异均有统计学意义(均P<0.05).结论 OLETF大鼠肺组织肌成纤维细胞生成增多,罗格列酮可抑制成纤维细胞向肌成纤维细胞的转化,早期改善糖尿病肺纤维化.
目的 探討自髮性2型糖尿病(OLETF)大鼠肺組織α-平滑肌肌動蛋白(α-SMA)錶達的變化及囉格列酮對其影響.方法 30週齡雄性OLETF大鼠16隻,分為OLETF組和OLETF/L組每組8隻,對照組為8隻LETO組大鼠.OLETF/L組大鼠給予囉格列酮3 mg/(kg·d)灌胃12週後處死大白鼠,應用免疫組織化學和免疫蛋白印跡法檢測各組大鼠肺組織α-SMA蛋白錶達.結果 各組大鼠肺組織α-SMA主要錶達于肌成纖維細胞、血管平滑肌細胞、小氣道平滑肌,OLETF組大鼠肺組織α-SMA蛋白錶達(2.03±0.64)明顯高于LETO組(1.18±0.35),P<0.05];OLETF/L組明顯減少(1.34±0.40),差異均有統計學意義(均P<0.05).結論 OLETF大鼠肺組織肌成纖維細胞生成增多,囉格列酮可抑製成纖維細胞嚮肌成纖維細胞的轉化,早期改善糖尿病肺纖維化.
목적 탐토자발성2형당뇨병(OLETF)대서폐조직α-평활기기동단백(α-SMA)표체적변화급라격렬동대기영향.방법 30주령웅성OLETF대서16지,분위OLETF조화OLETF/L조매조8지,대조조위8지LETO조대서.OLETF/L조대서급여라격렬동3 mg/(kg·d)관위12주후처사대백서,응용면역조직화학화면역단백인적법검측각조대서폐조직α-SMA단백표체.결과 각조대서폐조직α-SMA주요표체우기성섬유세포、혈관평활기세포、소기도평활기,OLETF조대서폐조직α-SMA단백표체(2.03±0.64)명현고우LETO조(1.18±0.35),P<0.05];OLETF/L조명현감소(1.34±0.40),차이균유통계학의의(균P<0.05).결론 OLETF대서폐조직기성섬유세포생성증다,라격렬동가억제성섬유세포향기성섬유세포적전화,조기개선당뇨병폐섬유화.
Objective To investigate the changes of expression of α—smooth muscle actin(α-SMA)in lung of spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty(OLETF)rats and the effect by tlle rosiglitazone.Methods Totally 16 male OLETF rats in 30 weeks old were divided into OLETF group and OLETF/L group.The Long Evans Tokushima Otsuka(LETO)rats were in control group.Thle OLtErI’F.rats were given gastric lavage bymsiglitazone 3 ms/(kg·d)then were killed after 12 weeks.The levels of ct-SMA in lung of three groups were detected by immunohistochemistry and western blotting.Results α-SMA in lung of three groups mainly expressed in myofibmblast.vascular smooth muscle cells and small airway smootII muscle.Compared to control group,tlle levels of α-SMA in lung of OIJETF group were increased obviously(2.03-4-0.64 VS 1.18 4-0.35,P<0.05).Compared to OLETF group,the levels of α-SMA in lung of OLETF/L group were decreased obviously(1.34 ±0.40 VS 2.03 ±0.64.P<0.05).Conclusion Myofibroblast increases in lung of OLETF rats.Rosiglitazone can inhibit the transformation from fibroblast to myofibroblast and improve pulmonary fibrosis in diabetes in early stage.