国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2011年
3期
75-79
,共5页
手足口病%肠道病毒%基因型%进化分析
手足口病%腸道病毒%基因型%進化分析
수족구병%장도병독%기인형%진화분석
Hand,foot and mouth disease%Enterovirus%Genotype%Phylogeny
目的 了解北京地区引起手足口病的非EV71、非CoxA16型肠道病毒的病原构成及优势型别VP4区遗传特征分析.方法 收集2009年4月-12月检测为非EV71、非Cox16型肠道病毒的手足口病患者咽拭子标本共45份.提取病毒核酸,用巢式RT-PCR法扩增病毒VP4区序列,对PCR阳性扩增产物进行测序,通过与GenBank收录的序列BLAST比对确定病毒型别;并对优势型别进行序列和进化分析.结果 45例标本中共有32例标本巢式RT-PCR检测为阳性(71%),其中26份PCR产物测序成功,经BLAST比对后,26例标本病毒型别均得到确定:CoxA10为11例;CoxA5为5例;CoxA12和CoxA4各2例;CoxA2、CoxA6、CoxA9、CoxA16、CoxB5、Echo11各1例.CoxA10在型别确定的非EV71、非CoxA16型肠道病毒中占44%,为优势肠道病毒型别.11株CoxA10在VP4区核苷酸序列同源性为82%-99%,氨基酸序列同源性为96%-100%.进化分析显示,10株北京株CoxA10与中国广东株在同一进化树分支上,1株与CoxA10日本株在同一分支上.结论 北京地区引起手足口病的肠道病毒型别多样,CoxA10为非EV71、非CoxA16型肠道病毒中的优势型别,北京地区CoxA10株包括两个不同的基因型.
目的 瞭解北京地區引起手足口病的非EV71、非CoxA16型腸道病毒的病原構成及優勢型彆VP4區遺傳特徵分析.方法 收集2009年4月-12月檢測為非EV71、非Cox16型腸道病毒的手足口病患者嚥拭子標本共45份.提取病毒覈痠,用巢式RT-PCR法擴增病毒VP4區序列,對PCR暘性擴增產物進行測序,通過與GenBank收錄的序列BLAST比對確定病毒型彆;併對優勢型彆進行序列和進化分析.結果 45例標本中共有32例標本巢式RT-PCR檢測為暘性(71%),其中26份PCR產物測序成功,經BLAST比對後,26例標本病毒型彆均得到確定:CoxA10為11例;CoxA5為5例;CoxA12和CoxA4各2例;CoxA2、CoxA6、CoxA9、CoxA16、CoxB5、Echo11各1例.CoxA10在型彆確定的非EV71、非CoxA16型腸道病毒中佔44%,為優勢腸道病毒型彆.11株CoxA10在VP4區覈苷痠序列同源性為82%-99%,氨基痠序列同源性為96%-100%.進化分析顯示,10株北京株CoxA10與中國廣東株在同一進化樹分支上,1株與CoxA10日本株在同一分支上.結論 北京地區引起手足口病的腸道病毒型彆多樣,CoxA10為非EV71、非CoxA16型腸道病毒中的優勢型彆,北京地區CoxA10株包括兩箇不同的基因型.
목적 료해북경지구인기수족구병적비EV71、비CoxA16형장도병독적병원구성급우세형별VP4구유전특정분석.방법 수집2009년4월-12월검측위비EV71、비Cox16형장도병독적수족구병환자인식자표본공45빈.제취병독핵산,용소식RT-PCR법확증병독VP4구서렬,대PCR양성확증산물진행측서,통과여GenBank수록적서렬BLAST비대학정병독형별;병대우세형별진행서렬화진화분석.결과 45례표본중공유32례표본소식RT-PCR검측위양성(71%),기중26빈PCR산물측서성공,경BLAST비대후,26례표본병독형별균득도학정:CoxA10위11례;CoxA5위5례;CoxA12화CoxA4각2례;CoxA2、CoxA6、CoxA9、CoxA16、CoxB5、Echo11각1례.CoxA10재형별학정적비EV71、비CoxA16형장도병독중점44%,위우세장도병독형별.11주CoxA10재VP4구핵감산서렬동원성위82%-99%,안기산서렬동원성위96%-100%.진화분석현시,10주북경주CoxA10여중국엄동주재동일진화수분지상,1주여CoxA10일본주재동일분지상.결론 북경지구인기수족구병적장도병독형별다양,CoxA10위비EV71、비CoxA16형장도병독중적우세형별,북경지구CoxA10주포괄량개불동적기인형.
Objectives To explore the pathogenic spectrum of hand,foot and mouth disease in Beijing and to analyse the genetic characteristics of the VP4 region of the predominant type of non-EV71,nonCoxA16 enteroviruses.Methods 45 HFMD specimens tested positive for enterovirus and negative for EV71and CoxA16 were collected from April to December of 2009.The vires RNA was extracted,and the VP4 sequence was amplified by nested RT-PCR.The PCR positive products were sequenced and the enterovirus type was identified by sequence Blast.The sequence and phylogenetic analysis of the predominant rirus type Was performed.Results 32 out of 45(71%)specimens were tested positive by nested RT-PCR,among which 26 PCR products were successfully sequenced.And the types of the 26 enterovirus strains were identified by sequence Blast:11 CoxA10,5 CoxA5,2 CoxA12,2CoxA4,1CoxA2,1CoxA6,1CoxA9,1CoxA16,1 CoxB5 and 1 Echo11.CoxA10 accounted for 44%in typed non-EV71,non-CoxA16 enteroviruses,and was the predominant type.The 11 CoxA10 strains shared the nucleotide identities of 82%-99%and the amino acid identities of 96%-100%with each other in VP4 region.Phylogenetic analysis showed that 10 CoxA10strains were clustered with Guangdong CoxA10 isolates and one clustered with Japan isolates.Conclusions Hand,foot and mouth disease in Beijing was concerned with diverse enteroviruses,and CoxA10 was the predominant type among non-EV71,non-CoxA16 enteroviruses;The CoxA10 strains isolated in Beijing could be clustered into two genotypes.
