CAJ | 학술논문

目的探讨超声联合微气泡开放大鼠血脑屏障的安全性及有效性.方法经大鼠尾静脉注射微气泡声诺维,同时采用GE Vivid 7超声辐射大鼠头颅,辐射深度经磁共振(MRI)扫描辅助定位于基底节区；以MRI增强扫描和荧光显微镜观察伊文思蓝的方法来检测血脑屏障开放程度,苏木素伊红(HE)染色观察细胞形态检测实验的安全性.结果超声联合微气泡辐射大鼠头颅后,在MRI增强扫描T1W1上观察到辐射区域信号增强,荧光显微镜下观察到辐射区域伊文思蓝的红色荧光；HE染色细胞形态正常,结构完整.结论超声联合微气泡可靶向、无创地开放大鼠血脑屏障,为中枢神经系统疾病的药物和干细胞治疗提供了新途径.
목적 탐토초성연합미기포개방대서혈뇌병장적안전성급유효성.방법 경대서미정맥주사미기포성낙유,동시채용GE Vivid 7초성복사대서두로,복사심도경자공진(MRI)소묘보조정위우기저절구；이MRI증강소묘화형광현미경관찰이문사람적방법래검측혈뇌병장개방정도,소목소이홍(HE)염색관찰세포형태검측실험적안전성.결과 초성연합미기포복사대서두로후,재MRI증강소묘T1W1상관찰도복사구역신호증강,형광현미경하관찰도복사구역이문사람적홍색형광；HE염색세포형태정상,결구완정.결론 초성연합미기포가파향、무창지개방대서혈뇌병장,위중추신경계통질병적약물화간세포치료제공료신도경.
Objective To explore the safety and effectiveness of diagnostic ultrasound associated with microbubbles to open the blood brain barrier(BBB).Methods Microbubbles were injected through caudal vein,the rat head was radiated by GE Vivid 7 diagnostic ultrasound immediately.The radiated depth was located in the basal ganglia assisted by magnetic resonance imaging(MRI)scanning.The degree of BBB opening was evaluated by enhanced MRI and Evans blue dyeing.The safety was inspected by observation of cell morphology under hematoxylin eosin(HE)staining.Results After the rat head radiated by diagnostic ultrasound with microbubbles,signal enhancement of the radiated area was observed on post contrast T1-weighted images.Red fluorescence of Evans blue was detected by fluorescence microscope in the same area.Normal cellular morphology and structural integrity were showed by HE staining.Conclusions The BBB of rat could be opened targetedly and noninvasively by diagnostic ultrasound associated with microbubbles.This may provide a new strategy for the drugs and stem cells treatment in the central nervous system diseases.