中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
3期
202-206
,共5页
卜强%汤华明%谈健%胡潇%王东文
蔔彊%湯華明%談健%鬍瀟%王東文
복강%탕화명%담건%호소%왕동문
前列腺肿瘤%RhoC%Rho激酶1%丝裂原活化蛋白激酶%Akt%浸润%转移
前列腺腫瘤%RhoC%Rho激酶1%絲裂原活化蛋白激酶%Akt%浸潤%轉移
전렬선종류%RhoC%Rho격매1%사렬원활화단백격매%Akt%침윤%전이
Prostatic neoplasms%RhoC%Rho kinase 1%Mitogen-activated protein kinase%Akt%Invasion%Metastasis
目的 检测RhoC和Rho激酶1(ROCK-1)在前列腺癌中的表达,探讨RhoC和ROCK-1在前列腺癌形成过程中的可能作用.方法 收集73例前列腺癌患者穿刺活检或手术切除的癌组织和对应的癌旁组织.采用逆转录聚合酶链反应(RT-PCR)检测RhoC mRNA和ROCK-1 mRNA表达,采用Western blot和免疫组化法检测RhoC和ROCK-1蛋白的表达.构建RhoC基因真核表达载体,转染前列腺癌不转移亚系PC-3M-2B4,并检测丝裂原活化蛋白激酶(MAPK)和Akt磷酸化水平变化.结果 前列腺癌组织中,RhoC mRNA和ROCK-1 mRNA的阳性率分别为72.6%(53/73)和68.5%(50/73),癌旁组织分别为34.2%(25/73)和38.4%(28/73),差异均有统计学意义(均P＜0.01).RhoC mRNA和ROCK-1 mRNA的表达与肿瘤转移呈正相关,而与肿瘤的Gleason分级无关.RhoC和ROCK-1蛋白在前列腺癌组织中的表达高于癌旁组织,并且与磷酸化MAPK和磷酸化Akt的表达呈正相关.携带RhoC基因的载体转染PC-3M-2B4细胞后,可以上调MAPK和Akt的磷酸化表达水平.结论 RhoC和ROCK-1在前列腺癌组织中的表达高于癌旁组织,且与肿瘤转移相关.RhoC和ROCK-1可能通过活化MAPK和Akt参与了前列腺癌的形成、浸润和转移.
目的 檢測RhoC和Rho激酶1(ROCK-1)在前列腺癌中的錶達,探討RhoC和ROCK-1在前列腺癌形成過程中的可能作用.方法 收集73例前列腺癌患者穿刺活檢或手術切除的癌組織和對應的癌徬組織.採用逆轉錄聚閤酶鏈反應(RT-PCR)檢測RhoC mRNA和ROCK-1 mRNA錶達,採用Western blot和免疫組化法檢測RhoC和ROCK-1蛋白的錶達.構建RhoC基因真覈錶達載體,轉染前列腺癌不轉移亞繫PC-3M-2B4,併檢測絲裂原活化蛋白激酶(MAPK)和Akt燐痠化水平變化.結果 前列腺癌組織中,RhoC mRNA和ROCK-1 mRNA的暘性率分彆為72.6%(53/73)和68.5%(50/73),癌徬組織分彆為34.2%(25/73)和38.4%(28/73),差異均有統計學意義(均P＜0.01).RhoC mRNA和ROCK-1 mRNA的錶達與腫瘤轉移呈正相關,而與腫瘤的Gleason分級無關.RhoC和ROCK-1蛋白在前列腺癌組織中的錶達高于癌徬組織,併且與燐痠化MAPK和燐痠化Akt的錶達呈正相關.攜帶RhoC基因的載體轉染PC-3M-2B4細胞後,可以上調MAPK和Akt的燐痠化錶達水平.結論 RhoC和ROCK-1在前列腺癌組織中的錶達高于癌徬組織,且與腫瘤轉移相關.RhoC和ROCK-1可能通過活化MAPK和Akt參與瞭前列腺癌的形成、浸潤和轉移.
목적 검측RhoC화Rho격매1(ROCK-1)재전렬선암중적표체,탐토RhoC화ROCK-1재전렬선암형성과정중적가능작용.방법 수집73례전렬선암환자천자활검혹수술절제적암조직화대응적암방조직.채용역전록취합매련반응(RT-PCR)검측RhoC mRNA화ROCK-1 mRNA표체,채용Western blot화면역조화법검측RhoC화ROCK-1단백적표체.구건RhoC기인진핵표체재체,전염전렬선암불전이아계PC-3M-2B4,병검측사렬원활화단백격매(MAPK)화Akt린산화수평변화.결과 전렬선암조직중,RhoC mRNA화ROCK-1 mRNA적양성솔분별위72.6%(53/73)화68.5%(50/73),암방조직분별위34.2%(25/73)화38.4%(28/73),차이균유통계학의의(균P＜0.01).RhoC mRNA화ROCK-1 mRNA적표체여종류전이정정상관,이여종류적Gleason분급무관.RhoC화ROCK-1단백재전렬선암조직중적표체고우암방조직,병차여린산화MAPK화린산화Akt적표체정정상관.휴대RhoC기인적재체전염PC-3M-2B4세포후,가이상조MAPK화Akt적린산화표체수평.결론 RhoC화ROCK-1재전렬선암조직중적표체고우암방조직,차여종류전이상관.RhoC화ROCK-1가능통과활화MAPK화Akt삼여료전렬선암적형성、침윤화전이.
Objective To detect the expression of RhoC and Rho kinase 1 ( ROCK-1 ) in prostate carcinoma, and explore the possible mechanism of RhoC/ROCK-1 in the pathogenesis of prostate carcinoma.Methods Tissue specimens from 73 patients with prostate carcinoma and corresponding paracancerous tissues were obtained by prostate cancer biopsy or radical prostatectomy. The expression of RhoC/ROCK-1 mRNA was detected by RT-PCR. Western blot and immunohistochemistry were performed to dertect the expression of RhoC/ROCK-1 protein. Eukaryotic expression plasmids of RhoC were constructed and transfected into PC-3M-2B4 cells. p-MAPK and p-Akt were detected by Western bolt. Results The expression levels of RhoC and ROCK-1 mRNA in the prostate carcinomas were significantly higher than those in corresponding paracancerous tissues [72.6% (53/73) vs. 34.2% (25/73) ;68.5 % (50/73) vs. 38.4%(28/73), P ＜0.01], respectively. The results indicated that RhoC/ROCK-1 mRNA expression had no significant correlation with Gleason grade. However, the expression of RhoC/ROCK-1 mRNA showed a significant positive correlation with distant metastasis. The RhoC/ROCK-1 protein expression in prostate cancer was also higher than corresponding paracancerous tissues, and showed a significant positive correlation with p-MAPK and p-Akt expression levels. In addition, p-MAPK and p-Akt expression levels were up-regulated in the transcripts. Conclusion Expression levels of RhoC and ROCK-1 in prostate carcinoma are higher than those in corresponding paracancerous tissues, showing a significant positive correlation with distant metastasis. RhoC/ROCK-1 may be involved in the development, invasion and metastasis of prostate carcinoma.
