CAJ | 학술논문

肉桂酰辅酶A还原酶(CCR)负责催化木质素单体生物合成中最重要的代谢反应,它将类苯丙酸类代谢物转移到木质素的合成途径中.为了更好地了解木质素在小麦生长发育中的作用,从小麦(Triticum aestivum L. cv. H4564)中克隆了两个肉桂酰辅酶A还原酶的cDNA.相似性和进化关系的分析表明这两个cDNA片段分别属于不同的肉桂酰辅酶A还原酶,这两个cDNA片段分别命名为W-cr6和W-cr19.RT-PCR和Northern杂交结果证明,W-cr6基因主要在小麦的茎和叶中表达,W-cr19基因主要在根和茎中表达.上述结果表明在小麦的基因组中至少存在两类肉桂酰辅酶A还原酶基因.
육계선보매A환원매(CCR)부책최화목질소단체생물합성중최중요적대사반응,타장류분병산류대사물전이도목질소적합성도경중.위료경호지료해목질소재소맥생장발육중적작용,종소맥(Triticum aestivum L. cv. H4564)중극륭료량개육계선보매A환원매적cDNA.상사성화진화관계적분석표명저량개cDNA편단분별속우불동적육계선보매A환원매,저량개cDNA편단분별명명위W-cr6화W-cr19.RT-PCR화Northern잡교결과증명,W-cr6기인주요재소맥적경화협중표체,W-cr19기인주요재근화경중표체.상술결과표명재소맥적기인조중지소존재량류육계선보매A환원매기인.
Cinnamoyl-CoA reductase (CCR) is responsible for the first committed reaction in monolignol biosynthesis, which diverts phenylpropanoid-derived metabolites into the biosynthesis of lignin. To gain a better understanding of the lignin biosynthesis in wheat development, two cDNAs encoding CCR were identified from wheat (Triticum aestivum L. cv. H4564). DNA sequence analyses indicated that the two cDNAs represent two classes of CCR. RT-PCR and Northern blot hybridization demonstrated that one of them, W-cr6, was expressed actively in stem and leaf tissue, the other one, W-cr19, was expressed in root and stem tissue. The results suggested that there are at least two genes encoded for CCR existing in wheat genome.