中国化学工程学报（英文版）
中國化學工程學報（英文版）
중국화학공정학보（영문판）
CHINESE JOURNAL OF CHEMICAL ENGINEERING
2007年
1期
122-126
,共5页
陈银%邢新会%叶逢春%况莹
陳銀%邢新會%葉逢春%況瑩
진은%형신회%협봉춘%황형
functional expression%fusion protein%green fluorescent protein (GFP)%heparinase Ⅰ%rapid quantification
To establish a rapid quantification method for heparinase Ⅰ dtring its production in recombinant Escherichia coli, a translational fusion vector was constructed by fusing the N terminus of heparinase Ⅰ to the C terminus of a green fluorescent protein mutant (GFPmut1). As a result, not only was the functional recombinant expression of heparinase Ⅰ in E. coli accomplished, but also a linear correlation was obtained between the GFP fluorescence intensity and heparinase Ⅰ activity, allowing enzyme activity to be quantified rapidly during the fermentation.
