CAJ | 학술논문

测定了我国长江水系和澜沧江水系的日本沼虾9个群体,共79个个体的线粒体COI基因序列片段(约450 bp),结果发现有89个变异位点,共计有46个单倍型.其中云南昆明(KM)群体具有较丰富的遗传多样性(h=1.000,π=0.028),而重庆(CQ)群体的遗传多样性最小(h=0.700,π=0.008).AMOVA分析表明,群体间的遗传变异占总遗传变异的9.66%,而90.34%的遗传变异源于群体内.采用邻接法(NJ)构建的分子系统树显示,46个单倍型明显地聚为长江中下游和长江上游与澜沧江两个族群.其结果可以为合理开发和利用日本沼虾自然野生资源,以及建立和保护日本沼虾种质资源库及基因库提供必要的参考.
측정료아국장강수계화란창강수계적일본소하9개군체,공79개개체적선립체COI기인서렬편단(약450 bp),결과발현유89개변이위점,공계유46개단배형.기중운남곤명(KM)군체구유교봉부적유전다양성(h=1.000,π=0.028),이중경(CQ)군체적유전다양성최소(h=0.700,π=0.008).AMOVA분석표명,군체간적유전변이점총유전변이적9.66%,이90.34%적유전변이원우군체내.채용린접법(NJ)구건적분자계통수현시,46개단배형명현지취위장강중하유화장강상유여란창강량개족군.기결과가이위합리개발화이용일본소하자연야생자원,이급건립화보호일본소하충질자원고급기인고제공필요적삼고.
This study analyzed nucleotide sequences from the mitochondrial cytochrome oxidase submit (COI) gene region (450bp) to investigate the genetic structure of the oriental river prawn (Macrobrachium nipponense) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h=1.000, π=0.028), whereas the population from Chongqing exhibits the lowest level of variability (h=0.700, π=0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (NJ) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.