CAJ | 학술논문

利用PCR-SSCP技术研究了南阳牛、秦川牛、郏县红牛、西镇牛、鲁西牛和荷斯坦奶牛6个牛品种539个个体leptin基因的遗传多态性.结果表明,PCR扩增产物大小为330 bp,PCR-SSCP分析表现出多态.南阳牛、秦川牛、郏县红牛、西镇牛、鲁西牛和荷斯坦奶牛的A等位基因频率分别为0.558,0.492,0.571,0.658,0.591,0.615;B等位基因频率分别为0.442,0.508,0.429,0.342,0.409,0.385.不同基因型与体重、体尺等生长性状指标相关性分析的结果表明:南阳牛群体内除12月龄的体高和日增重、18月龄的坐骨端宽和日增重外,BB型个体的六月龄、十二月龄、十八月龄、二十四月龄体斜长、胸围、体重、坐骨端宽、体高和日增重均显著的大于AB和AA型个体(P＜0.05);秦川牛群体内BB基因型个体十字部高上显著高于群体AA、AB型个体(P＜0.05),即BB＞AA、AB,可作为秦川牛体尺指标(十字部高)候选基因之一,但在体重、胸围、体长指标上均无显著差异(P＞0.05),所以不宜作为体重、胸围、体长指标候选基因;郏县红牛群体内AB与BB基因型个体在十字部高和坐骨端宽上显著高于群体AA型个体(P＜0.05),而群体内不同基因型在体重和体尺指标(体高、体斜长、胸围)上无显著差异(P＞0.05).序列分析表明,leptin基因多态是第66位发生G→T、第67位发生A→C及299位发生新的单核苷酸突变C→T所造成.
이용PCR-SSCP기술연구료남양우、진천우、겹현홍우、서진우、로서우화하사탄내우6개우품충539개개체leptin기인적유전다태성.결과표명,PCR확증산물대소위330 bp,PCR-SSCP분석표현출다태.남양우、진천우、겹현홍우、서진우、로서우화하사탄내우적A등위기인빈솔분별위0.558,0.492,0.571,0.658,0.591,0.615;B등위기인빈솔분별위0.442,0.508,0.429,0.342,0.409,0.385.불동기인형여체중、체척등생장성상지표상관성분석적결과표명:남양우군체내제12월령적체고화일증중、18월령적좌골단관화일증중외,BB형개체적륙월령、십이월령、십팔월령、이십사월령체사장、흉위、체중、좌골단관、체고화일증중균현저적대우AB화AA형개체(P＜0.05);진천우군체내BB기인형개체십자부고상현저고우군체AA、AB형개체(P＜0.05),즉BB＞AA、AB,가작위진천우체척지표(십자부고)후선기인지일,단재체중、흉위、체장지표상균무현저차이(P＞0.05),소이불의작위체중、흉위、체장지표후선기인;겹현홍우군체내AB여BB기인형개체재십자부고화좌골단관상현저고우군체AA형개체(P＜0.05),이군체내불동기인형재체중화체척지표(체고、체사장、흉위)상무현저차이(P＞0.05).서렬분석표명,leptin기인다태시제66위발생G→T、제67위발생A→C급299위발생신적단핵감산돌변C→T소조성.
PCR-SSCP was used to analyze the polymorphism of leptin gene in 539 samples of six cattle breeds, namely Nanyang (NY), Qinchuan (QC), Jiaxianred (JXR), Xizben (XZ), Luxi (LX), and Holstein cow (HOL) breeds. PCR products with a 330 bp were amplified and sequenced. The results showed that the frequencies of alleles A/B of NY, QC, JXR, XZ, LX, and HOL breeds were 0.558/0.442, 0.492/0.508, 0.571/0.429, 0.658/0.342, 0.591/0.409, and 0.615/0.385, respectively. The association of variations of leptin gene with growth traits in NY, QC, JXR breeds was analyzed. Some indexes of the individuals with genotype BB were higher than that with genotype AA and AB in NY breed, such as the indexes of body length, heart length, body weight, hucklebone width, body height, and average day gain. The height at hip cross of the individuals with genotype BB was higher than that of those with genotype AA and AB in QC breed (P ＜ 0.05). So leptin gene may be one of the candidate genes for growth traits with height at hip cross, but not for body weight, heart length, and body length trait. However, the height at hip cross and hucklebone width of the individuals with genotype AB and BB were higher than that of those with genotype AA in JXR breed (P ＜ 0.05), but the difference was not statistically significant in body weight and body sizes (body height, body length, and heart length). And the polymorphisms in leptin gene were caused by G→T transversion at the 66th bp position, A→C transversion at the 67th bp position and G→T transversion at the 299th bp position. These results may be applied to marker-assisted selection of Chinese cattle breeds.