中国环境科学
中國環境科學
중국배경과학
CHINA ENVIRONMENTAL SCIENCE
2010年
2期
209-215
,共7页
高大文%李昕芯%安瑞%付源%任南琪
高大文%李昕芯%安瑞%付源%任南琪
고대문%리흔심%안서%부원%임남기
膜生物反应嚣%溶解氧%PCR-DGGE%微生物群落结构%氨氧化菌
膜生物反應囂%溶解氧%PCR-DGGE%微生物群落結構%氨氧化菌
막생물반응효%용해양%PCR-DGGE%미생물군락결구%안양화균
membrane bioreactor (MBR)%dissolved oxygen%PCR-DGGE%microbial community structure%ammonia oxidizing bacteria
应用A/O-MBR处理实际生活污水.考察了不同溶解氧(DO)条件下,微生物群落结构与其处理效果的对应关系.结果表明,DO浓度在0.2~4.0mg/L对COD去除效果无明显影响,COD平均去除率均在90%以上.DO浓度变化对NH_4~+-N去除影响较大,DO浓度下降到0.2mg/L时,NH_4~+-N平均去除率由99%下降到65%.通过PCR-DGGE分析,较高DO条件下(4.0,2.0mg/L)的总细菌微生物群落多样性高于较低DO条件(0.5,0.2mg/L),但其群落结构变化与与反应器的处理效果对应关系不明显;氨氧化菌的群落结构变化较明显,且在不同的DO条件下起主要作用的氨氧化菌的菌属不同,其群落变化与反应器的NH_4~+-N去除效果相对戍,DO为2.0,0.5mg/L时氨氧化菌群落结构比较相似,此时反应系统的脱氮效粜也比较好.
應用A/O-MBR處理實際生活汙水.攷察瞭不同溶解氧(DO)條件下,微生物群落結構與其處理效果的對應關繫.結果錶明,DO濃度在0.2~4.0mg/L對COD去除效果無明顯影響,COD平均去除率均在90%以上.DO濃度變化對NH_4~+-N去除影響較大,DO濃度下降到0.2mg/L時,NH_4~+-N平均去除率由99%下降到65%.通過PCR-DGGE分析,較高DO條件下(4.0,2.0mg/L)的總細菌微生物群落多樣性高于較低DO條件(0.5,0.2mg/L),但其群落結構變化與與反應器的處理效果對應關繫不明顯;氨氧化菌的群落結構變化較明顯,且在不同的DO條件下起主要作用的氨氧化菌的菌屬不同,其群落變化與反應器的NH_4~+-N去除效果相對戍,DO為2.0,0.5mg/L時氨氧化菌群落結構比較相似,此時反應繫統的脫氮效糶也比較好.
응용A/O-MBR처리실제생활오수.고찰료불동용해양(DO)조건하,미생물군락결구여기처리효과적대응관계.결과표명,DO농도재0.2~4.0mg/L대COD거제효과무명현영향,COD평균거제솔균재90%이상.DO농도변화대NH_4~+-N거제영향교대,DO농도하강도0.2mg/L시,NH_4~+-N평균거제솔유99%하강도65%.통과PCR-DGGE분석,교고DO조건하(4.0,2.0mg/L)적총세균미생물군락다양성고우교저DO조건(0.5,0.2mg/L),단기군락결구변화여여반응기적처리효과대응관계불명현;안양화균적군락결구변화교명현,차재불동적DO조건하기주요작용적안양화균적균속불동,기군락변화여반응기적NH_4~+-N거제효과상대수,DO위2.0,0.5mg/L시안양화균군락결구비교상사,차시반응계통적탈담효조야비교호.
Microbial community structure and nutrient removal performance under different dissolved oxygen were investigated using Anoxic-oxic submerged membrane bioreactor (A/O-MBR) for municipal wastewater treatment. Results showed that the COD removal was not influenced markedly by the variations in DO between 0.2mg/L and 4.0mg/L, and the COD removal was more than 90% during the whole experiment. However, the ammonia nitrogen removal was more sensitive than COD, and the ammonia nitrogen removal dropped from 99% to 65% when DO decreased to 0.2mg/L. The PCR-DGGE analysis showed that the diversity of total bacterial community was abundant when A/O-MBR was run at high DO (4.0mg/L and 2.0mg/L), and the relationships between the total haeterial community structure and the performance of MBR was not very obvious. On the contrary, the ammonia oxidizing bacterial community composition had a more obvious shift with the variation of DO concentration, and the species of ammonia oxidizing bacteria which play major role were different under different DO conditions, which were corresponded to the removal efficiency. The ammonia oxidizing bacterial community composition were similar under the DO concentration of 2.0mg/L and 0.5mg/L, and the nitrogen renmoval was enhanced.