CAJ | 학술논문

背景与目的:调节性T细胞(Treg)在肿瘤组织局部浸润增多,而对其来源报道较少.体外研究表明转化生长因子(transforming growth factor-β1,TGF-β1)能诱导Treg的产生,而肝癌组织中TGF-β1表达与Treg浸润的关系尚未阐明.本研究旨在检测肝癌组织中TGF-β1表达,并分析其与Treg浸润的关系,探讨TGF-β1表达,Treg浸润的临床意义.方法:采用免疫组织化学染色的方法检测102例肝癌组织、癌旁肝组织中TGF- β1和Foxp3表达(Foxp3+).结果:肝癌组织中TGF-β1低表达组4l例,高表达组6l例,高表达阳性率为59.8%;癌旁组织中TGF-β1低表达组22例,高表达组80例,高表达阳性率为78.4%,癌组织和癌旁高表达率差异有统计学意义(P= 0.001).肝癌组织中Foxp3+细胞平均密度为2.98个／HP, 癌旁极少见或未见.肝癌组织中TGF-β1与Foxp3表达呈正相关(r=0.228,P=0.021).肝癌组织中TGF-β1表达在术前血浆AFP浓度高水平组高于低水平组(P=0.023),而与肿瘤直径、肿瘤包膜、肝硬化等临床病理资料无关;肝癌组织中Foxp3表达与肿瘤直径、肿瘤包膜、肝硬化等临床病理资料无关.肝癌组织中TGF-β1表达高低水平组患者5年生存率差异无统计学意义(P=0.790);肝癌组织中Treg高水平组患者的5年生存率低于低水平组(25%vs.44%,P< 0.005).多因素cox回归模型分析显示Foxp3+细胞数量、肿瘤包膜是影响肝癌患者预后的独立危险因素(P值分别为 0.021、0.001).结论:肝癌组织中Treg浸润可能与TGF-β1 表达有关,两者的关系需进一步研究;肝癌组织中Treg数量可作为预测患者术后预后的免疫学指标.
배경여목적:조절성T세포(Treg)재종류조직국부침윤증다,이대기래원보도교소.체외연구표명전화생장인자(transforming growth factor-β1,TGF-β1)능유도Treg적산생,이간암조직중TGF-β1표체여Treg침윤적관계상미천명.본연구지재검측간암조직중TGF-β1표체,병분석기여Treg침윤적관계,탐토TGF-β1표체,Treg침윤적림상의의.방법:채용면역조직화학염색적방법검측102례간암조직、암방간조직중TGF- β1화Foxp3표체(Foxp3+).결과:간암조직중TGF-β1저표 체조4l례,고표체조6l례,고표체양성솔위59.8%;암방조 직중TGF-β1저표체조22례,고표체조80례,고표체양성솔 위78.4%,암조직화암방고표체솔차이유통계학의의(P= 0.001).간암조직중Foxp3+세포평균밀도위2.98개／HP, 암방겁소견혹미견.간암조직중TGF-β1여Foxp3표체정정 상관(r=0.228,P=0.021).간암조직중TGF-β1표체재 술전혈장AFP농도고수평조고우저수평조(P=0.023),이 여종류직경、종류포막、간경화등림상병리자료무관;간암조 직중Foxp3표체여종류직경、종류포막、간경화등림상병리 자료무관.간암조직중TGF-β1표체고저수평조환자5년생 존솔차이무통계학의의(P=0.790);간암조직중Treg고수 평조환자적5년생존솔저우저수평조(25%vs.44%,P< 0.005).다인소cox회귀모형분석현시Foxp3+세포수량、종 류포막시영향간암환자예후적독립위험인소(P치분별위 0.021、0.001).결론:간암조직중Treg침윤가능여TGF-β1 표체유관,량자적관계수진일보연구;간암조직중Treg수량 가작위예측환자술후예후적면역학지표.
Background and Objective: There are few studies about origins of regulatory T(Treg)cells increased in primary hepatocellular carcinoma(HCC)tissue.Studies showed that Treg cells could be induced by transforming growth factor-β1(TGF-β1),but the relation of TGF-β1expression and Treg cell infiltration is unclear in HCC tissue.This study was to investigate the expression of TGF-β1 and correlation between the expression of TGF-β1 and the amount of Treg cells in HCC,and to evaluate their clinical values in predicting the prognosis of HCC.Methods: EnVision immunohistochemistry was used to detect the expression of TGF-β1 and Foxp3 in 102 specimens of HCC tissue and paired adjacent non-tumor liver tissue.Results:of the 102 specimens of HCC,41 showed low TGF-β1expression and 61(59.8%)showed high expression; of the 102 specimens of adjacent non-tumor tissue,22 showed low TGF-β1 expression and 80(78.4%)showed high expression.The high expression rate of TGF-β1 was significantly lower in HCC than in adjacent non-tumor tissues(P=0.001).Average Foxp3+ cell density in HCC was 2.98 cells/HP,but there was very few or no expression of Foxp3 in adjacent non-tumor liver tissue.Expression of TGF-β1 was positively correlated with expression of Foxp3 in HCC tissuezs(r=0.228,P=0.021).The expression of TGF-β1 was significantly higher in HCC tissues with high preoperative AFP concentration than in those with low preoperative AFP concentration(P=0.023).TGF-β1 and Foxp3 expression had no correlations with tumor diameter,tumor capsule,liver cirrhosis,and so on.The 5-year survival rate was not different between HCC tissues with high and low TGF-β1 expression(P=0.790).It was significantly lower in HCC tissues with high Treg cell infiltration than in those with low infiltration(25% vs.44%,P=0.007).Cox multivariate analysis showed that the number of Treg cells and tumor capsule were independent prognostic factors(P=0.021,P=0.001).Conclusions: Expression of TGF-β1 may relate to the infiltration of Treg cells in HCC tissues,but the relation need to be further investigated.The number of Treg cells in HCC tissues could be used as.a potential immunological prognostic indicator for HCC patients after resection.