CAJ | 학술논문

从福建省平潭岛野生坛紫菜群体中发现有患细菌性红烂病的叶状体,从患病的叶状体上分离得到病原细菌一株,经16S rDNA序列分析发现其与细菌域海螺菌目(Oceanospirillales)、盐单胞菌科(Halomonadaceae)、Cobetia属的海科贝特菌(Cobetia marina)的相似度达100 %.病原细菌经液体培养基扩大培养后,将适量的菌液接种到不同种紫菜和坛紫菜不同品系的叶状体圆盘块上进行人工回复感染试验以确定其感染特性.结果发现该病原细菌能感染健康野生坛紫菜且出现的病症与海区患病坛紫菜相同,且该病原细菌对不同种紫菜(坛紫菜、条斑紫菜、未定名紫菜)的叶状体圆盘块和不同品系的坛紫菜(AN-2,YZ-6,JIU-7,ZS-1)叶状体圆盘块均能快速感染,所产生的病症与野生坛紫菜叶状体感染红烂病的症状相同:均出现了铁锈红色的死亡细胞,且同时含有少量被解离的单离细胞.上述结果说明分离到的病原细菌是坛紫菜红烂病的病原细菌,可以快速感染各种不同的紫菜叶状体.
종복건성평담도야생단자채군체중발현유환세균성홍란병적협상체,종환병적협상체상분리득도병원세균일주,경16S rDNA서렬분석발현기여세균역해라균목(Oceanospirillales)、염단포균과(Halomonadaceae)、Cobetia속적해과패특균(Cobetia marina)적상사도체100 %.병원세균경액체배양기확대배양후,장괄량적균액접충도불동충자채화단자채불동품계적협상체원반괴상진행인공회복감염시험이학정기감염특성.결과발현해병원세균능감염건강야생단자채차출현적병증여해구환병단자채상동,차해병원세균대불동충자채(단자채、조반자채、미정명자채)적협상체원반괴화불동품계적단자채(AN-2,YZ-6,JIU-7,ZS-1)협상체원반괴균능쾌속감염,소산생적병증여야생단자채협상체감염홍란병적증상상동:균출현료철수홍색적사망세포,차동시함유소량피해리적단리세포.상술결과설명분리도적병원세균시단자채홍란병적병원세균,가이쾌속감염각충불동적자채협상체.
A strain of pathogenic bacterium was isolated from the blades of wild type Porphyra haitanensis with red-rotting disease collected from the wild population along the coasts of Pingtan Island of Fujian Province. The bacterium had a 100% similarity with Cobetia marina (Oceanospirillales, Proteobacteria) according to analysis of the 16S rDNA array. After the pathogenic bacterium was cultured in liquid medium for a period of time, an amount of bacterial suspension was inoculated onto the blades of different Porphyra in an artificial re-infection. It was found that the isolated bacterium could infect the healthy wild type P.haitanensis blades with the same symptoms to the diseased blades in the field, and the different species of Porphyra (P.haitanensis, Porphyra yezoensis, and Porphyra sp.) blades and different strains of P.haitanensis (AN-2, YZ-6, JIU-7, ZS-1) blades were all infected rapidly with the same symptoms to the wild type P.haitanensis blades in which the infected cells were in rust color and several single cells were enzymatically decomposed from the blades. It was concluded that the bacterium isolated was the pathogen of red-rotting disease of P.haitanensis blades and could also infect blades of Porphyra spp. rapidly.