中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2010年
8期
478-480
,共3页
贾利宁%田普训%薛武军%丁小明%潘晓鸣%项和立%靳占奎
賈利寧%田普訓%薛武軍%丁小明%潘曉鳴%項和立%靳佔奎
가리저%전보훈%설무군%정소명%반효명%항화립%근점규
免疫球蛋白样转录物3%免疫球蛋白样转录物4%肾移植%免疫低反应性
免疫毬蛋白樣轉錄物3%免疫毬蛋白樣轉錄物4%腎移植%免疫低反應性
면역구단백양전록물3%면역구단백양전록물4%신이식%면역저반응성
Immunoglobulin-like transcripts 3%Immunoglobulin-like transcripts 4%Kidney transplantation%Immune hyporesponsiveness
目的 探讨免疫球蛋白样转录物3和4(ILT3和ILT4)在肾移植受者外周血树突状细胞(DC)中的表达情况,并分析其在移植免疫低反应中的作用.方法 选择肾移植后存活5年以上肾功能稳定和慢性排斥反应的受者各10例,分别作为肾功能稳定组和慢性排斥组;另选择10例健康志愿者作为正常对照组.用粒-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素4(IL-4)刺激各组研究对象的外周血单核细胞,诱导培养获得DC.用流式细胞仪测定DC表面分子ILT3和ILT4的表达,并分析各组DC表达的差异;用酶联免疫吸附试验法测定各组血清中ILT3和ILT4的配体HLA-G5的表达水平.结果 ILT3在肾功能稳定组DC中表达水平上调,与正常对照组和慢性排斥组比较,差异有统计学意义(P＜0.05),而ILT4的表达在各组间比较,差异无统计学意义(P＞0.05);肾功能稳定组血清中HLA-G5的表达水平较正常对照组和慢性排斥组显著升高,差异有统计学意义(P＜0.05).结论 长期存活的肾功能稳定受者外周血中高表达ILT3和其配体HLA-G,说明它们可能在外周免疫耐受的诱导和维持中起非常重要的作用.
目的 探討免疫毬蛋白樣轉錄物3和4(ILT3和ILT4)在腎移植受者外週血樹突狀細胞(DC)中的錶達情況,併分析其在移植免疫低反應中的作用.方法 選擇腎移植後存活5年以上腎功能穩定和慢性排斥反應的受者各10例,分彆作為腎功能穩定組和慢性排斥組;另選擇10例健康誌願者作為正常對照組.用粒-巨噬細胞集落刺激因子(GM-CSF)和白細胞介素4(IL-4)刺激各組研究對象的外週血單覈細胞,誘導培養穫得DC.用流式細胞儀測定DC錶麵分子ILT3和ILT4的錶達,併分析各組DC錶達的差異;用酶聯免疫吸附試驗法測定各組血清中ILT3和ILT4的配體HLA-G5的錶達水平.結果 ILT3在腎功能穩定組DC中錶達水平上調,與正常對照組和慢性排斥組比較,差異有統計學意義(P＜0.05),而ILT4的錶達在各組間比較,差異無統計學意義(P＞0.05);腎功能穩定組血清中HLA-G5的錶達水平較正常對照組和慢性排斥組顯著升高,差異有統計學意義(P＜0.05).結論 長期存活的腎功能穩定受者外週血中高錶達ILT3和其配體HLA-G,說明它們可能在外週免疫耐受的誘導和維持中起非常重要的作用.
목적 탐토면역구단백양전록물3화4(ILT3화ILT4)재신이식수자외주혈수돌상세포(DC)중적표체정황,병분석기재이식면역저반응중적작용.방법 선택신이식후존활5년이상신공능은정화만성배척반응적수자각10례,분별작위신공능은정조화만성배척조;령선택10례건강지원자작위정상대조조.용립-거서세포집락자격인자(GM-CSF)화백세포개소4(IL-4)자격각조연구대상적외주혈단핵세포,유도배양획득DC.용류식세포의측정DC표면분자ILT3화ILT4적표체,병분석각조DC표체적차이;용매련면역흡부시험법측정각조혈청중ILT3화ILT4적배체HLA-G5적표체수평.결과 ILT3재신공능은정조DC중표체수평상조,여정상대조조화만성배척조비교,차이유통계학의의(P＜0.05),이ILT4적표체재각조간비교,차이무통계학의의(P＞0.05);신공능은정조혈청중HLA-G5적표체수평교정상대조조화만성배척조현저승고,차이유통계학의의(P＜0.05).결론 장기존활적신공능은정수자외주혈중고표체ILT3화기배체HLA-G,설명타문가능재외주면역내수적유도화유지중기비상중요적작용.
Objective To study the expression of immunoglobulin-like transcripts 3 (ⅡT3) and ILT4 in peripheral blood dendritic cells (DC) of kidney transplantation recipients and to analyze its significance in immunity hyporesponsiveness of transplantation. Methods Twenty kidney allograft recipients who were survived more than five years were recruited to two groups: renal function stable groups, chronic rejection groups, and 10 healthy volunteers served as a control group. The peripheral blood mononuclear cells (PBMC) were stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) and immature DC were obtained. The expression of ILT3 and ILT4 was detected by using flow cytometry. The level of HLA-G5 in serum was determined by using enzyme linked immunosorbent assay. Results ILT3 expression in renal function stable group was increased and decreased in chronic rejection groups as compared with control group (P＜0.05),but ILT4 expression had no significant difference among all groups. HLA-G5 in serum was significantly increased in renal function stable group as compared with other groups. Conclusion Expression of ILT3 and HLA-G was increased in the kidney transplantation recipients with stable renal function and long-term survival, suggesting that they may play an important role in inducing and maintaining periphery immune tolerance.
