CAJ | 학술논문

目的对安阳地区的食管鳞癌新鲜手术标本和石蜡标本进行HPV检测,比较两种不同标本来源食管鳞癌HPV感染检测结果,统计学分析二者之间的差异,并同其他区域检测结果进行比较,分析不同标本对检测结果的影响.方法对安阳的23例新鲜手术标本及23例石蜡标本分别进行核酸提取;分别用通用引物PCR鉴定,并用人乳头瘤病毒核酸扩增分型检测试剂盒对样品进行分型检测;比较两种标本来源检测结果并进行统计分析;比较分析检测结果与其他地区检测结果的差异.结果经检测新鲜标本HPV感染率为82.6％,其中HPV16型感染率34.8％,HPV18型感染率34.8％.石蜡包埋组织标本HPV感染率78.2％,其中HPV16型感染率30.4％,HPV18型感染率17.4％.结论首次证明两种来源标本对HPV检测结果无显著性影响,均可作为检测HPV的样本,为HPV科研检测提供更广的标本来源.
목적 대안양지구적식관린암신선수술표본화석사표본진행HPV검측,비교량충불동표본래원식관린암HPV감염검측결과,통계학분석이자지간적차이,병동기타구역검측결과진행비교,분석불동표본대검측결과적영향.방법 대안양적23례신선수술표본급23례석사표본분별진행핵산제취;분별용통용인물PCR감정,병용인유두류병독핵산확증분형검측시제합대양품진행분형검측;비교량충표본래원검측결과병진행통계분석;비교분석검측결과여기타지구검측결과적차이.결과 경검측신선표본HPV감염솔위82.6％,기중HPV16형감염솔34.8％,HPV18형감염솔34.8％.석사포매조직표본HPV감염솔78.2％,기중HPV16형감염솔30.4％,HPV18형감염솔17.4％.결론 수차증명량충래원표본대HPV검측결과무현저성영향,균가작위검측HPV적양본,위HPV과연검측제공경엄적표본래원.
Objective Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions.Methods Extracted the total DNA from the novel fresh tissue and Paraffinembedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio) ; Compared the statistical result from the different specimens; analyzed the result between different region.Results HPV infection rate of fresh tissue is 82.6％ with HPV16 (34.8％ ) and HPV18 ( 34.8％ ),and paraffinembedded specimens is 78.2％ with HPV16 ( 30.4％ ) and HPV18 ( 17.4％ ).Conclusion The results provides the first evidence that there wasn' t noticeable difference between HPV infection rate of the two specimens.So broader specimen source could be used for HPV testing.