中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2008年
3期
171-174
,共4页
万福生%李国辉%章洁%余乐涵%赵小曼
萬福生%李國輝%章潔%餘樂涵%趙小曼
만복생%리국휘%장길%여악함%조소만
烧伤%心肌%线粒体,心脏%牛磺酸
燒傷%心肌%線粒體,心髒%牛磺痠
소상%심기%선립체,심장%우광산
Burns%Myocardium%Mitochondria,heart%Taurine
目的 了解牛磺酸对大鼠烫伤后心肌线粒体成分和酶活性变化的影响.方法 将120只大鼠背部脱毛后造成30%TBSA的Ⅲ度烫伤.分为烫伤组(60只),伤后腹腔注射等渗盐水(4mL·kg-1·1%TBSA-1);牛磺酸治疗组(60只),伤后腹腔注射20 g/L牛磺酸液(200 mg/kg).伤后1、3、6、12、24、48 h处死大鼠.取心肌组织检测心肌线粒体琥珀酸脱氢酶(SDH)、细胞色素c氧化酶(CCO)、超氧化物歧化酶(SOD)、Ca2+-腺苷三磷酸酶(ATPase)活性和细胞色素c、细胞色素aa3、丙二醛(MDA)、细胞质及线粒体内ca2+含量.另取10只大鼠作为对照组,模拟烫伤不予补液,1 h后处死大鼠取心肌组织检测以上指标.结果 烫伤组大鼠伤后1 h CCO活性即开始下降,伤后6、12 h下降最明显,而SDH活性在伤后6 h下降最明显,且各时相点均低于对照组;牛磺酸治疗组CCO和SDH下降不明显,伤后3、6、12 h CCO活性与烫伤组比较,差异有统计学意义(P＜0.05).伤后3、6、12、及24 h烫伤组心肌线粒体细胞色素c和细胞色素aa3均显著降低.而牛磺酸组虽然有所下降,但以上4个时相点的值均高于烫伤组(P＜0.05).烫伤组SOD活性在伤后3、6、12 h均显著性下降,ca2+ATPase活性在伤后3、6、12及24 h均有不同程度下降;而牛磺酸治疗组虽然有所下降,但以上4个时相点SOD、Ca2+-ATPase值均高于烫伤组(P＜0.05).伤后3～48 h烫伤组、牛磺酸治疗组MDA值均高于对照组(P＜0.05),但牛磺酸治疗组低于烫伤组(P＜0.05).伤后1 h烫伤组大鼠心肌线粒体中Ca2+即开始升高为13.7±1.5,伤后3、6、12、24 h分别为24.8±2.6、29.7±3.1、16.3±1.9及13.5±1.7.均高于对照组(10.7±1.6,P＜0.05);心肌细胞质中ca2+在伤后3、6、12、24 h也明显高于对照组(P＜0.05).伤后3、6、12及24 h牛磺酸治疗组大鼠心肌线粒体中ca2+浓度分别为16.8±2.8、18.7±1.9、10.5±1.8及13.3±1.7,均低于烫伤组.结论 牛磺酸对烫伤大鼠心肌线粒体成分和酶活性破坏具有保护作用,其机制与提高线粒体清除氧自由基的能力和减轻Ca2+超载有关.
目的 瞭解牛磺痠對大鼠燙傷後心肌線粒體成分和酶活性變化的影響.方法 將120隻大鼠揹部脫毛後造成30%TBSA的Ⅲ度燙傷.分為燙傷組(60隻),傷後腹腔註射等滲鹽水(4mL·kg-1·1%TBSA-1);牛磺痠治療組(60隻),傷後腹腔註射20 g/L牛磺痠液(200 mg/kg).傷後1、3、6、12、24、48 h處死大鼠.取心肌組織檢測心肌線粒體琥珀痠脫氫酶(SDH)、細胞色素c氧化酶(CCO)、超氧化物歧化酶(SOD)、Ca2+-腺苷三燐痠酶(ATPase)活性和細胞色素c、細胞色素aa3、丙二醛(MDA)、細胞質及線粒體內ca2+含量.另取10隻大鼠作為對照組,模擬燙傷不予補液,1 h後處死大鼠取心肌組織檢測以上指標.結果 燙傷組大鼠傷後1 h CCO活性即開始下降,傷後6、12 h下降最明顯,而SDH活性在傷後6 h下降最明顯,且各時相點均低于對照組;牛磺痠治療組CCO和SDH下降不明顯,傷後3、6、12 h CCO活性與燙傷組比較,差異有統計學意義(P＜0.05).傷後3、6、12、及24 h燙傷組心肌線粒體細胞色素c和細胞色素aa3均顯著降低.而牛磺痠組雖然有所下降,但以上4箇時相點的值均高于燙傷組(P＜0.05).燙傷組SOD活性在傷後3、6、12 h均顯著性下降,ca2+ATPase活性在傷後3、6、12及24 h均有不同程度下降;而牛磺痠治療組雖然有所下降,但以上4箇時相點SOD、Ca2+-ATPase值均高于燙傷組(P＜0.05).傷後3～48 h燙傷組、牛磺痠治療組MDA值均高于對照組(P＜0.05),但牛磺痠治療組低于燙傷組(P＜0.05).傷後1 h燙傷組大鼠心肌線粒體中Ca2+即開始升高為13.7±1.5,傷後3、6、12、24 h分彆為24.8±2.6、29.7±3.1、16.3±1.9及13.5±1.7.均高于對照組(10.7±1.6,P＜0.05);心肌細胞質中ca2+在傷後3、6、12、24 h也明顯高于對照組(P＜0.05).傷後3、6、12及24 h牛磺痠治療組大鼠心肌線粒體中ca2+濃度分彆為16.8±2.8、18.7±1.9、10.5±1.8及13.3±1.7,均低于燙傷組.結論 牛磺痠對燙傷大鼠心肌線粒體成分和酶活性破壞具有保護作用,其機製與提高線粒體清除氧自由基的能力和減輕Ca2+超載有關.
목적 료해우광산대대서탕상후심기선립체성분화매활성변화적영향.방법 장120지대서배부탈모후조성30%TBSA적Ⅲ도탕상.분위탕상조(60지),상후복강주사등삼염수(4mL·kg-1·1%TBSA-1);우광산치료조(60지),상후복강주사20 g/L우광산액(200 mg/kg).상후1、3、6、12、24、48 h처사대서.취심기조직검측심기선립체호박산탈경매(SDH)、세포색소c양화매(CCO)、초양화물기화매(SOD)、Ca2+-선감삼린산매(ATPase)활성화세포색소c、세포색소aa3、병이철(MDA)、세포질급선립체내ca2+함량.령취10지대서작위대조조,모의탕상불여보액,1 h후처사대서취심기조직검측이상지표.결과 탕상조대서상후1 h CCO활성즉개시하강,상후6、12 h하강최명현,이SDH활성재상후6 h하강최명현,차각시상점균저우대조조;우광산치료조CCO화SDH하강불명현,상후3、6、12 h CCO활성여탕상조비교,차이유통계학의의(P＜0.05).상후3、6、12、급24 h탕상조심기선립체세포색소c화세포색소aa3균현저강저.이우광산조수연유소하강,단이상4개시상점적치균고우탕상조(P＜0.05).탕상조SOD활성재상후3、6、12 h균현저성하강,ca2+ATPase활성재상후3、6、12급24 h균유불동정도하강;이우광산치료조수연유소하강,단이상4개시상점SOD、Ca2+-ATPase치균고우탕상조(P＜0.05).상후3～48 h탕상조、우광산치료조MDA치균고우대조조(P＜0.05),단우광산치료조저우탕상조(P＜0.05).상후1 h탕상조대서심기선립체중Ca2+즉개시승고위13.7±1.5,상후3、6、12、24 h분별위24.8±2.6、29.7±3.1、16.3±1.9급13.5±1.7.균고우대조조(10.7±1.6,P＜0.05);심기세포질중ca2+재상후3、6、12、24 h야명현고우대조조(P＜0.05).상후3、6、12급24 h우광산치료조대서심기선립체중ca2+농도분별위16.8±2.8、18.7±1.9、10.5±1.8급13.3±1.7,균저우탕상조.결론 우광산대탕상대서심기선립체성분화매활성파배구유보호작용,기궤제여제고선립체청제양자유기적능력화감경Ca2+초재유관.
Objective To investigate the effects of taurine on myocardial mitochondria and their enzyme activities in rats with severe burn. Methods One hundred and twenty healthy adult Wistar rats were subjeeted to 30%TBSA full-thickness burn.They were randomly divided into bum group(B,with intraper-itoneal injection of isotonic saline),treatment group(T,with intraperitoneal injection of taurine,200 mg/kg),with 60 rats in each group.Ten rats with sham scald were used as control(S group).The myocardial tissue samples in B and T groups were harvested at 1,3,6,12,24 and 48 postburn hours(PBH)for deter-mintion of activity respectively of succinate dehydrogenase(SDH),cytochrame oxidase(CCO),the superox-ide dismutase(SOD),Ca2+-ATPase in mitochondria,and contents of cytochrome c(Cyt c),cytochrome aa3(Cyt aa3),malondlaldehyde(MDA).and Ca2+ in mitochondria and cytoplasm.The myocardial tissue samples of controls were harvested at 1 PBH for determintion of above indices. Results The activity of CCO in B group was decreased at 1 PBH,especially at 6.12 PBH.The activity of SDH in B group was de-creased to Jowest level at 6 PBH.and its value was lower than that of S group at each time point.The activity 0f CCO or SDH in T group was not obviously decreased.and the activity of CCO at 3,6,12 PBH showed signif-icant difference compared with B group(P＜0.05).The contents of Cyt aa3 and Cyt c in B group at 3,6,12,24PBH were obviously decreased,which were significantly lower than those in T group(P＜0.05).The activity of SOD in B group at 3,6,12 PBH was obviously decreased,the activity of Ca2+-ATPase at 3,6,12 and 24 PBH was decreased to difierent extent,which was significantly lower than those in T group(P＜0.05).The MDA contents in B and T groups were higher than that in S group at 3～48 PBH,and it was highest in B group(P＜0.05).The Ca2+ content of mitochondria in B group at 1 PBH wag increased (13.7±1.5),and it was(24.8±2.6),(29.7±3.1),(16.3±1.9)and(13.5±1.7)at 3,6,12,24PBH respectively,and they were all higher than that of S group(10.7±1.6,P＜0.05).The Ca2+ contents of cytoplasm in group B at 3～24 PBH were also higher than that in S group(P＜0.05).The Ca2+ con-tent of mitochondria in T group at 3,6,12,24 PBH was(16.8±2.8),(18.7±1.9),(10.5±1.8)and (13.3±1.7)respectively,which were lower than that in B group at every time point. Conclusion Tau-rine have protective effect on mitochondria and their enzyme activities in myocardium in rats with severe burn,and it may be attributable to improvimg the ability of eradicating oxygen free radicals and alleviating Ca2+ overload in the mitochondria.
