CAJ | 학술논문

目的检测结核杆菌抗原早期分泌抗原靶分子(ESAT-6)融合蛋白其在人类免疫缺陷病毒感染患者中特异性T细胞反应,从而为艾滋病合并结核杆菌感染的诊断方法的建立提供理论依据.方法利用重组表达结核杆菌ESAT-6蛋白做为抗原,通过酶联免疫斑点技术(ELISPOT)检测HIV感染者中特异性T细胞反应,并以正常健康者、无HIV感染的结核患者作为对照.结果 ESAT-6重组蛋白对结核患者外周血单核细胞ELISPOT方法检测证实其具有良好的抗原性,斑点形成单位数(SFU/106 PBMC)在结核组和HIV合并结核感染组中显著升高,并与无合并结核感染临床表现的HIV组以及健康对照组间均存在明显的差别.结论重组结核杆菌ESAT-6蛋白可以作为抗原通过ELISPOT方法检测HIV感染者中结核杆菌合并感染.
목적 검측결핵간균항원조기분비항원파분자(ESAT-6)융합단백기재인류면역결함병독감염환자중특이성T세포반응,종이위애자병합병결핵간균감염적진단방법적건립제공이론의거.방법이용중조표체결핵간균ESAT-6단백주위항원,통과매련면역반점기술(ELISPOT)검측HIV감염자중특이성T세포반응,병이정상건강자、무HIV감염적결핵환자작위대조.결과 ESAT-6중조단백대결핵환자외주혈단핵세포ELISPOT방법검측증실기구유량호적항원성,반점형성단위수(SFU/106 PBMC)재결핵조화HIV합병결핵감염조중현저승고,병여무합병결핵감염림상표현적HIV조이급건강대조조간균존재명현적차별.결론중조결핵간균ESAT-6단백가이작위항원통과ELISPOT방법검측HIV감염자중결핵간균합병감염.
Objective To detect TB specific T cell responses by using the recombinant ESAT-6 protein as stimulus in Chinese HIV infected patients. Methods ELISPOT-IFN-γ assay by using the recombinant ESAT-6protein as stimulus to detect specific T cell responses in HIV( + ) patients with or without clinical manifestation of TB diseases. Results Recombinant ESAT-6 protein specific T cell responses show significant high frequencies in both of TB patients with or without HIV infection than that in the healthy control and HIV ( + ) group without clinical TB diseases. Conclusions The ELISPOT-IFN-γ assay by using recombinant ESAT-6 protein as stimulus could be used in diagnoses of TB infection in Chinese HIV infected patients.