中华手外科杂志
中華手外科雜誌
중화수외과잡지
CHINESE JOURNAL OF HAND SURGERY
2009年
2期
107-109
,共3页
骆渊城%康皓%李进%陈燕花%胡克力%洪光祥
駱淵城%康皓%李進%陳燕花%鬍剋力%洪光祥
락연성%강호%리진%진연화%호극력%홍광상
神经再生%脊髓%神经节,脊%热休克蛋白质类%银杏叶提取物EGb 761
神經再生%脊髓%神經節,脊%熱休剋蛋白質類%銀杏葉提取物EGb 761
신경재생%척수%신경절,척%열휴극단백질류%은행협제취물EGb 761
Nerve regeneration%Spinal cord%Ganglia,spinal%Heat shock proteins%Ginkgohiloha extract EGb 761
目的 研究周围神经损伤后脊髓热休克蛋白(heat shock proteirns,HSPs)的表达变化,及银杏叶提取物EGb 761对其影响,探讨银杏叶提取物对周围神经损伤的保护机制.方法 取成年雄性SD大鼠144只,随机分成三组:对照组、坐骨神经切断组、坐骨神经切断+银杏叶提取物干预组[术后每天用EGb 761(100 mg·kg-1*d-1,溶于2 ml SAL)灌胃,直到取材],每组48只.术后6 h、12 h、1 d.2 d,4 d、7 d、14 d、28 d取L4～6节段脊髓节段,采用免疫组织化学方法检测HSP 70在脊髓前角及脊神经节中的表达.结果 正常大鼠脊髓与神经节中均有少量HSP 70表达,在坐骨神经切断后表达迅速增加,持续一段时间后又回到正常水平,用EGb 761干预后,HSP 70表达早期较未干预组表达低,后期表达增高,且表达持续时间长.结论 银杏叶提取物EGb 761可以增加神经损伤后HSP 70表达,可能是银杏叶提取物保护神经,促进神经再生作用的机制.
目的 研究週圍神經損傷後脊髓熱休剋蛋白(heat shock proteirns,HSPs)的錶達變化,及銀杏葉提取物EGb 761對其影響,探討銀杏葉提取物對週圍神經損傷的保護機製.方法 取成年雄性SD大鼠144隻,隨機分成三組:對照組、坐骨神經切斷組、坐骨神經切斷+銀杏葉提取物榦預組[術後每天用EGb 761(100 mg·kg-1*d-1,溶于2 ml SAL)灌胃,直到取材],每組48隻.術後6 h、12 h、1 d.2 d,4 d、7 d、14 d、28 d取L4～6節段脊髓節段,採用免疫組織化學方法檢測HSP 70在脊髓前角及脊神經節中的錶達.結果 正常大鼠脊髓與神經節中均有少量HSP 70錶達,在坐骨神經切斷後錶達迅速增加,持續一段時間後又迴到正常水平,用EGb 761榦預後,HSP 70錶達早期較未榦預組錶達低,後期錶達增高,且錶達持續時間長.結論 銀杏葉提取物EGb 761可以增加神經損傷後HSP 70錶達,可能是銀杏葉提取物保護神經,促進神經再生作用的機製.
목적 연구주위신경손상후척수열휴극단백(heat shock proteirns,HSPs)적표체변화,급은행협제취물EGb 761대기영향,탐토은행협제취물대주위신경손상적보호궤제.방법 취성년웅성SD대서144지,수궤분성삼조:대조조、좌골신경절단조、좌골신경절단+은행협제취물간예조[술후매천용EGb 761(100 mg·kg-1*d-1,용우2 ml SAL)관위,직도취재],매조48지.술후6 h、12 h、1 d.2 d,4 d、7 d、14 d、28 d취L4～6절단척수절단,채용면역조직화학방법검측HSP 70재척수전각급척신경절중적표체.결과 정상대서척수여신경절중균유소량HSP 70표체,재좌골신경절단후표체신속증가,지속일단시간후우회도정상수평,용EGb 761간예후,HSP 70표체조기교미간예조표체저,후기표체증고,차표체지속시간장.결론 은행협제취물EGb 761가이증가신경손상후HSP 70표체,가능시은행협제취물보호신경,촉진신경재생작용적궤제.
Objective To study the expression of heat shock protein 70 (HSP 70) in spinal cord and dorsal root ganglia after peripheral nerve injury and the influence of Ginkgo biloba extract EGb 761 on HSP 70 expression, and investigate the mechanism of the protective effect of EGb 761 after peripheral nerve injury in rats. Methods One hundred and forty-four male adult SD mrs were randomly divided into three groups, sham operation group ( n= 48), sciatic nerve transection group ( n = 48) and sciatic nerve transection and EGb 761 hours, 12 hours, 1 day, 2 days, 4 days, 7 days, 14 days and 28 days postoperatively, spinal cord of L4-L6 were harvested. Immunohistochemistry analysis was done to measure the expression of HSP 70 in anterior horns of Lt-L6 spinal cord and dorsal root ganglia. Results In normal rats' spinal cord anterior horn and dorsal root ganglia, HSP 70 was expressed. After rat sciatic nerve transection, the expression of HSP 70 increased quickly.This elevated expression lasted for a period of time, and then returned to the normal level. After being intervened by EGb 761, HSP 70 expression during the early postoperative period was lower compared with the group not being intervened. At the later period, the expression increased and lasted for a longer time. Conclusion Ginkgo biloba extract( EGb 761 ) can enhance the expression of HSP 70 after peripheral nerve injury. This could be the mechanism of the neuroprotective and neurotrophic effect of Ginkgo biloha extract.
