肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
12期
799-801
,共3页
任连生%张蕻%白喜花%韩雪冰%米振国
任連生%張蕻%白喜花%韓雪冰%米振國
임련생%장홍%백희화%한설빙%미진국
苏木精%膀胱肿瘤%细胞系,肿瘤%细胞凋亡
囌木精%膀胱腫瘤%細胞繫,腫瘤%細胞凋亡
소목정%방광종류%세포계,종류%세포조망
Hematoxylin%Urinary bladder neoplasms%Cell line,tumor%,Apoptosis
目的 观察苏木精对人类膀胱癌T24细胞的杀伤及诱导凋亡的作用,探讨其对靶细胞杀伤的作用机制.方法 将靶细胞培养于含药量分别为0、12.5、25、50、100、200μg/ml的RPMI1640培养基中,培养24h,倒置显微镜下观察细胞的形态学变化,收集靶细胞,采用锥虫蓝拒染法测定药物对靶细胞的杀伤作用.采用流式细胞术(FCM)检测不同药物质量浓度对靶细胞凋亡的影响.结果 随着药物质量浓度的逐步增加,细胞发生形态学变化,且细胞死亡率逐步增加,对照组细胞死亡率为(2.63±0.29)%,各组细胞死亡率分别为(10.00±4.82)%、(21.88±3.42)%、(76.41±4.82)%、(92.27±7.62)%和(96.34±8.78)%.对照组细胞凋亡的自然发生率为0.47%;含药量为50μg/ml时,细胞凋亡率显著增高,达到43.18%,死细胞率为48.47%,活细胞率为8.35%;随着药物质量浓度的增大.细胞凋亡率逐渐降低.而死细胞率则逐渐上升.结论 苏木精可通过诱导细胞凋亡和直接杀伤作用于靶细胞,在较低浓度下,可诱导靶细胞发牛凋亡,药物浓度超过100μg/ml时,则能直接杀死靶细胞.
目的 觀察囌木精對人類膀胱癌T24細胞的殺傷及誘導凋亡的作用,探討其對靶細胞殺傷的作用機製.方法 將靶細胞培養于含藥量分彆為0、12.5、25、50、100、200μg/ml的RPMI1640培養基中,培養24h,倒置顯微鏡下觀察細胞的形態學變化,收集靶細胞,採用錐蟲藍拒染法測定藥物對靶細胞的殺傷作用.採用流式細胞術(FCM)檢測不同藥物質量濃度對靶細胞凋亡的影響.結果 隨著藥物質量濃度的逐步增加,細胞髮生形態學變化,且細胞死亡率逐步增加,對照組細胞死亡率為(2.63±0.29)%,各組細胞死亡率分彆為(10.00±4.82)%、(21.88±3.42)%、(76.41±4.82)%、(92.27±7.62)%和(96.34±8.78)%.對照組細胞凋亡的自然髮生率為0.47%;含藥量為50μg/ml時,細胞凋亡率顯著增高,達到43.18%,死細胞率為48.47%,活細胞率為8.35%;隨著藥物質量濃度的增大.細胞凋亡率逐漸降低.而死細胞率則逐漸上升.結論 囌木精可通過誘導細胞凋亡和直接殺傷作用于靶細胞,在較低濃度下,可誘導靶細胞髮牛凋亡,藥物濃度超過100μg/ml時,則能直接殺死靶細胞.
목적 관찰소목정대인류방광암T24세포적살상급유도조망적작용,탐토기대파세포살상적작용궤제.방법 장파세포배양우함약량분별위0、12.5、25、50、100、200μg/ml적RPMI1640배양기중,배양24h,도치현미경하관찰세포적형태학변화,수집파세포,채용추충람거염법측정약물대파세포적살상작용.채용류식세포술(FCM)검측불동약물질량농도대파세포조망적영향.결과 수착약물질량농도적축보증가,세포발생형태학변화,차세포사망솔축보증가,대조조세포사망솔위(2.63±0.29)%,각조세포사망솔분별위(10.00±4.82)%、(21.88±3.42)%、(76.41±4.82)%、(92.27±7.62)%화(96.34±8.78)%.대조조세포조망적자연발생솔위0.47%;함약량위50μg/ml시,세포조망솔현저증고,체도43.18%,사세포솔위48.47%,활세포솔위8.35%;수착약물질량농도적증대.세포조망솔축점강저.이사세포솔칙축점상승.결론 소목정가통과유도세포조망화직접살상작용우파세포,재교저농도하,가유도파세포발우조망,약물농도초과100μg/ml시,칙능직접살사파세포.
Objective To observe hematoxylin's cytocidal and apoptosis-inducing effects on human urinary cancer cell-T24,and its cytocidal mechanism to the target cell.Methods Target cells were incubated in the medium 1640 for 24h,which contained hematoxylin in dosage of zero(blank),12.5,25,50,100,200μg/ml,respectively;under inverted microscopy to observe target cells'morphologic change,and then harvest them;by trypan blue tmpochrome method to determine hematoxylin's cytocidal activity to the target cells;by flow cytomelry to detect the effects of hematoxylin in its different levels on target cells'apoptosis.Results The control group(without hematoxylin)showed their target cells in a fusiform adherent growth,plump,close-arranged,and with a good transparence.With the addition and increment of hematoxylin,target cells turned round,not adherent,pyknotic,with a bad transparence,as well as chromatin condensation,the cells clumped.Cell death rate of control group was(2.63±0.29)%,with the increased dosage of hematoxylin the cell death rate of test groups was(10.00±4.82)%,(21.88±3.42)%,(76.41±4.82)%,(92.27±6.54)%,and(96.34±8.70)%respectively.Flow cytometry showed cell apoptosis rate in control group was 0.47%(occurred spontaneously),but hematoxylin in dose of 50μg/ml made the apoptosis rate increased markedly,to 43.1 8%,dead cell rate 48.47%,and survival cell rate 8.35%.With the increased hematoxylin dose,cell apoptosis rate decreased gradually,while dead cell rate increased.Conclusion Hematoxylin can inhibit the target cell by two routes:to induce apoptose or kill it.In a lower dose it is able to induce target cell to apeptose;hematoxylin in a dose over 100μg/ml can directly kill the target cell.Making this trial for checking the cell's morphologic changes benefits determining the optimal dosage level and optimal acting duration for the apoptosis induction.
