CAJ | 학술논문

目的研究有活性的激酶C受体(RACK1)在食管鳞癌组织中的表达水平及其临床意义.方法 Western印迹检测食管鳞癌细胞中RACKI的表达.免疫组织化学方法检测组织芯片中113例食管鳞癌组织及其配对的癌旁正常食管黏膜中RACKl和Ki67蛋白的表达水平.将病例按年龄、性别、肿瘤部位、吸烟与否、分化程度和TNM分期进行分组,比较不同组别中RACKl表达水平的差异.结果 RACKl在食管鳞癌组织中的表达低于癌旁正常食管黏膜,差异具有统计学意义(x2=63.363,P<0.01).不吸烟组的肿瘤组织中RACKl高表达率为72.5%(29/40),高于吸烟组的46.6%(34/73)(X2=7.040,P=0.008).TNM l期和Ⅱ期患者的肿瘤组织中RACKl高表达率为63.8%(44/69),高于TNMⅢ期的43.2%(19/44)(0=4.616,P=0.032).Ki67评分越高的肿瘤组织中RACKl的表达越低(x2=8.261,P=0.016).结论 RACKl在食管鳞癌中表达发生下调.食管鳞癌中RACKI的表达与吸烟、TNM分期及Ki67评分具有相关性.
목적 연구유활성적격매C수체(RACK1)재식관린암조직중적표체수평급기림상의의.방법 Western인적검측식관린암세포중RACKI적표체.면역조직화학방법검측조직심편중113례식관린암조직급기배대적암방정상식관점막중RACKl화Ki67단백적표체수평.장병례안년령、성별、종류부위、흡연여부、분화정도화TNM분기진행분조,비교불동조별중RACKl표체수평적차이.결과 RACKl재식관린암조직중적표체저우암방정상식관점막,차이구유통계학의의(x2=63.363,P<0.01).불흡연조적종류조직중RACKl고표체솔위72.5%(29/40),고우흡연조적46.6%(34/73)(X2=7.040,P=0.008).TNM l기화Ⅱ기환자적종류조직중RACKl고표체솔위63.8%(44/69),고우TNMⅢ기적43.2%(19/44)(0=4.616,P=0.032).Ki67평분월고적종류조직중RACKl적표체월저(x2=8.261,P=0.016).결론 RACKl재식관린암중표체발생하조.식관린암중RACKI적표체여흡연、TNM분기급Ki67평분구유상관성.
Objective To explore the expression level and clinical significance of receptor for activated C kinase 1(RACKl)in esophageal squamous cell carcinoma(ESCC).Methods Western Blotting was performed to detect the RACKl expression in ESCC cell lines.Immunohistochemistry was conducted to assay the expression of RACKl and Ki67 in tumor tissues and adjacent normal epithelium from 113 ESCC patients in tissue microarray.The relationship between the RACKI level and such clinieopathologic profiles as age,gender,location,smoking,differentiation degree and TNM(tumor,node,metastasis)stage were analyzed.Results The expression of RACKl protein was significantly downregulated in ESCC tissues as compared with the normal adjacent epithelium(X2=63.363,P<0.01).An upregulated expression of RACKl was observed in 72.5%(29/40)ESCC tissues of patients without a smoking history.And it was significantly higher than that in 46.6%(34/73)of patients with a smoking history(x2=7.040,P=0.008).In addition,the rate of up-regulated of RACKl was significantly higher in stage I andⅡgroup(63.8%,44/69)than that in stage Ⅲ group(43.2%,19/44)(x2=4.616,P=0.032).Moreover.the ESCC tissues with a higher Ki67 score showed a lower level of RACKl than that with a lower Ki67 score(x2=8.261,P=0.016).Conclusions The expression of RACKl is down-regulated in ESCC tissues and it is associated with smoking,The expression of RACKl was associated with smoking,TNM staging and Ki67 score of ESCC.