中国媒介生物学及控制杂志
中國媒介生物學及控製雜誌
중국매개생물학급공제잡지
CHINESE JOURNAL OF VECTOR BIOLOGY AND CONTROL
2001年
2期
122-124
,共3页
陈素良%张亚利%郭逸秀%周立强%李春明%李桂银%师鉴%李月平%孔令义%徐吉和%甄素娟
陳素良%張亞利%郭逸秀%週立彊%李春明%李桂銀%師鑒%李月平%孔令義%徐吉和%甄素娟
진소량%장아리%곽일수%주립강%리춘명%리계은%사감%리월평%공령의%서길화%견소연
恙虫病%血清学%间接免疫荧光法
恙蟲病%血清學%間接免疫熒光法
양충병%혈청학%간접면역형광법
Tsutsugamushi disease%Serology%Indirect immunofluorescent
目的：对河北省恙虫病疫源地内病例进行血清学分型和抗体动态观察，了解疫源地和非疫源地内人群恙虫病抗体阳性率，初步掌握恙虫病血清流行病学规律。方法：采集患者病后不同时期的血清标本和疫源地内及非疫源地内人群血清标本，用间接免疫荧光法检测恙虫病抗体。结果：27例恙虫病患者均被证实为Gilliam血清型。病后第3d即可出现IgM和IgG抗体，20d至1年IgM和IgG抗体阳性率均为100%，抗体滴度高峰为病后10～19d，之后开始逐渐下降。疫源地内健康人群IgG和IgM抗体阳性率分别为7.70%和4.81%，提示可能存在隐性感染。非疫源地内未发现抗体阳性者。结论：初步阐明恙虫病的血清流行病学特点。在流行区可能存在隐性感染。
目的：對河北省恙蟲病疫源地內病例進行血清學分型和抗體動態觀察，瞭解疫源地和非疫源地內人群恙蟲病抗體暘性率，初步掌握恙蟲病血清流行病學規律。方法：採集患者病後不同時期的血清標本和疫源地內及非疫源地內人群血清標本，用間接免疫熒光法檢測恙蟲病抗體。結果：27例恙蟲病患者均被證實為Gilliam血清型。病後第3d即可齣現IgM和IgG抗體，20d至1年IgM和IgG抗體暘性率均為100%，抗體滴度高峰為病後10～19d，之後開始逐漸下降。疫源地內健康人群IgG和IgM抗體暘性率分彆為7.70%和4.81%，提示可能存在隱性感染。非疫源地內未髮現抗體暘性者。結論：初步闡明恙蟲病的血清流行病學特點。在流行區可能存在隱性感染。
목적：대하북성양충병역원지내병례진행혈청학분형화항체동태관찰，료해역원지화비역원지내인군양충병항체양성솔，초보장악양충병혈청류행병학규률。방법：채집환자병후불동시기적혈청표본화역원지내급비역원지내인군혈청표본，용간접면역형광법검측양충병항체。결과：27례양충병환자균피증실위Gilliam혈청형。병후제3d즉가출현IgM화IgG항체，20d지1년IgM화IgG항체양성솔균위100%，항체적도고봉위병후10～19d，지후개시축점하강。역원지내건강인군IgG화IgM항체양성솔분별위7.70%화4.81%，제시가능존재은성감염。비역원지내미발현항체양성자。결론：초보천명양충병적혈청류행병학특점。재류행구가능존재은성감염。
Objective:To obtain Rickettsia tsutsugamushi(R.t.) antibody levelin patients and healthy population of epidemic area and non-epidemic area, and to get the seroepidemiological pattern of tsutsugamushi disease in Hebei province based on serological typing and dynamic observation.Methods:Serum specimens of different time series after the onset of tsutsugamushi disease were collected from patients. Serum specimens were also collected from healthy population in epidemic area and non-epidemic area. Indirect immunofluorescence were carried out to detect Rt antibody and to identify the serological type. Results:Gilliam serological type was identified in all of the 27 patients. R.t. IgG and IgM antibodies could be detected at 3 days after the onset of disease. And positive rate of both Rt IgG and IgM antibodies were 100% from 20 days to 1 year after the onset of disease. The titers of antibodies reached the peak point at 10-19 days after the onset of disease, and then declined gradually. Positive rates of R.t. IgG and IgM were 7.70% and 4.81% in healthy population of epidemic area, respectively. And the antibodies were not detected in healthy population of non-epidemic area. Conclusion:Seroepidemiological pattern of tsutsugamushi disease was clarified primarily. In epidemic area, recessive infection might exist.
