CAJ | 학술논문

目的探讨不同浓度的持续高糖和间断高糖对牛视网膜血管周细胞(BRPs)增殖、凋亡及细胞周期的影响.方法分别在低糖、持续及间断高糖下培养BRPs 7天;应用MTT比色法观察各组BRPs增殖情况;应用TUNEL法检测BRPs凋亡率;应用流式细胞术观察BRPs细胞周期的情况.结果 (1)持续及间断高糖均町抑制BRPs增殖,诱导其凋亡,与对照组比较,P<0.01,其中35mmol/L持续高糖组的吸光度为0.274,凋亡率为48.73%;(2)持续及间断高糖可使BRPs G0/G1期细胞比例增加,S期细胞比例减少,G2/M细胞比例无明显改变;(3)间断高糖组中波动幅度及频度大者对BRPs的作用更明显.结论高糖毒性和高糖波动性共同参与视网膜病变的发生,且高糖绝对水平的作用超过高糖的不稳定性作用.
목적 탐토불동농도적지속고당화간단고당대우시망막혈관주세포(BRPs)증식、조망급세포주기적영향.방법 분별재저당、지속급간단고당하배양BRPs 7천;응용MTT비색법관찰각조BRPs증식정황;응용TUNEL법검측BRPs조망솔;응용류식세포술관찰BRPs세포주기적정황.결과 (1)지속급간단고당균정억제BRPs증식,유도기조망,여대조조비교,P<0.01,기중35mmol/L지속고당조적흡광도위0.274,조망솔위48.73%;(2)지속급간단고당가사BRPs G0/G1기세포비례증가,S기세포비례감소,G2/M세포비례무명현개변;(3)간단고당조중파동폭도급빈도대자대BRPs적작용경명현.결론 고당독성화고당파동성공동삼여시망막병변적발생,차고당절대수평적작용초과고당적불은정성작용.
Objective To study the effects of constant and intermittent high glucose on the proliferation,apoptosis and cell cycle of bovine retinal capillary pericytes(BRPs). Methods BRPs were cultured under low glucose、constant high glucose and intermittent high glucose respectively for a week.The proliferation of BRPs was assayed with MTT assay. The apoptosis of BRPs was studied by in-situ TUNEL method. The cell cycles of BRPs was analyzed by flow cytometry. Results (1) Constant high glucose and intermittent high glucose could inhibit the proliferation and induce apoptosis of BRPs,as compared with control group(P<0.01).(2) Both constant and intermittent high glucose increased the percentage of G1 phase and decreased the percentage of S phase. But there was no obvious change in the percentage of G2/M phase ratio in each group. (3) The constant high glucose which had bigger range and frequency of the fluctuation had a stronger effect on BRPs. Conclusions Both high glucose toxicity and fluctuant high glucose involve in the development of diabetic retinopathy. And the absolute level of high glucose plays a main role in the development of diabetic retinopathy