世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2009年
7期
476-479
,共4页
王志慧%杨小洁%樊飞%李兴%赵跃斌
王誌慧%楊小潔%樊飛%李興%趙躍斌
왕지혜%양소길%번비%리흥%조약빈
2型糖尿病%糖尿病肾病%瘦素
2型糖尿病%糖尿病腎病%瘦素
2형당뇨병%당뇨병신병%수소
Type 2 diabetes mellitus%Diabetic nephropathy%Leptin
目的 探讨瘦素在糖尿病肾病发病(DN)中的作用,以期为DN的早期诊治提供一定参考价值.方法 雄性Wistar大鼠随机分为对照组和实验组,对照组喂以普通饲料2个月后,腹腔注射等体积的枸橼酸缓冲液;实验组喂以高糖高脂饲料2个月后,腹腔注射STZ 30 mg/kg.分别测定各组血清瘦素(leptin)、空腹胰岛素(FINS)水平.结果 ①实验组大鼠经高热量饮食2个月及注射STZ后各阶段血糖、胆固醇、甘油三酯均显著高于对照组(P＜0.01);②实验组大鼠FINS及胰岛素抵抗指数在高热量饮食2个月至注射STZ 6个月内持续存在;③实验组大鼠于注射STZ 2个月后出现明显的蛋白尿;④实验组大鼠肾小球体积于注射STZ 1个月后,体积开始增大;而在3个月及6个月时肾小球体积则显著下降;⑤实验组大鼠血清瘦素浓度在高热量饮食2个月后较对照组显著增高(P＜0.01).结论 leptin与DN的发生、发展有密切联系.
目的 探討瘦素在糖尿病腎病髮病(DN)中的作用,以期為DN的早期診治提供一定參攷價值.方法 雄性Wistar大鼠隨機分為對照組和實驗組,對照組餵以普通飼料2箇月後,腹腔註射等體積的枸櫞痠緩遲液;實驗組餵以高糖高脂飼料2箇月後,腹腔註射STZ 30 mg/kg.分彆測定各組血清瘦素(leptin)、空腹胰島素(FINS)水平.結果 ①實驗組大鼠經高熱量飲食2箇月及註射STZ後各階段血糖、膽固醇、甘油三酯均顯著高于對照組(P＜0.01);②實驗組大鼠FINS及胰島素牴抗指數在高熱量飲食2箇月至註射STZ 6箇月內持續存在;③實驗組大鼠于註射STZ 2箇月後齣現明顯的蛋白尿;④實驗組大鼠腎小毬體積于註射STZ 1箇月後,體積開始增大;而在3箇月及6箇月時腎小毬體積則顯著下降;⑤實驗組大鼠血清瘦素濃度在高熱量飲食2箇月後較對照組顯著增高(P＜0.01).結論 leptin與DN的髮生、髮展有密切聯繫.
목적 탐토수소재당뇨병신병발병(DN)중적작용,이기위DN적조기진치제공일정삼고개치.방법 웅성Wistar대서수궤분위대조조화실험조,대조조위이보통사료2개월후,복강주사등체적적구연산완충액;실험조위이고당고지사료2개월후,복강주사STZ 30 mg/kg.분별측정각조혈청수소(leptin)、공복이도소(FINS)수평.결과 ①실험조대서경고열량음식2개월급주사STZ후각계단혈당、담고순、감유삼지균현저고우대조조(P＜0.01);②실험조대서FINS급이도소저항지수재고열량음식2개월지주사STZ 6개월내지속존재;③실험조대서우주사STZ 2개월후출현명현적단백뇨;④실험조대서신소구체적우주사STZ 1개월후,체적개시증대;이재3개월급6개월시신소구체적칙현저하강;⑤실험조대서혈청수소농도재고열량음식2개월후교대조조현저증고(P＜0.01).결론 leptin여DN적발생、발전유밀절련계.
Objective To explore the role of leptin in diabetic nephropathy(DN)so as to provide a certain reference for the early diagnosis and treatment of DN.Methods Male Wistar rats were divided randomly into control group and experimental group.In control group,after feeding with common forage for 2 months,citric acid buffer of equal volume was injected intraperitoneally.In experimental group,after feeding with high-sugar and high-fat forage for 2 months,streptozotocin(STZ)30 mg/kg was injected intraperitoneally.Serum leptin and fasting insulin(FINS)were determined in each group.Results ①The levels of blood glucose,cholesterol and triglyceride(TG)at different stages after injection of STZ in rats of experimental group after 2-month feeding of high caloric forage were all higher significantly than those in control group(P＜0.01).②FINS and insulin resistance index of rats in experimental group were persistent in 6 months since STZ injection after 2-month feeding of high caloric forage.③Protein urine was apparent in 2 months after STZ injection in rats of experimental group.④The volume of glomerule in rats of experimental group started increasing 1 month after STZ injection and decreased significantly in 3 months and 6 months of injection.⑤The concentration of leptin in rats of experimental group after 2-month high calorie feeding increased significantly compared with control group(P＜0.01).Conclusion Leptin is closely related with the occurrence and development of DN.
