厦门大学学报（自然科学版）
廈門大學學報（自然科學版）
하문대학학보（자연과학판）
JOURNAL OF XIAMEN UNIVERSITY (NATURAL SCIENCE)
2010年
1期
95-101
,共7页
东海原甲藻%链状亚历山大藻%硝酸盐%氨盐%硝酸还原酶%谷氨酰胺合成酶
東海原甲藻%鏈狀亞歷山大藻%硝痠鹽%氨鹽%硝痠還原酶%穀氨酰胺閤成酶
동해원갑조%련상아력산대조%초산염%안염%초산환원매%곡안선알합성매
Prorocentrum donghaiense%Alexandrium catenella%nitrate%ammonium%nitrate reductase%glutamine synthetase
比较研究了硝酸盐和氨盐培养下,两种甲藻(东海原甲藻(Prorocentrum donghaiense Lu)和链状亚历山大藻(Alexandrium catenella DH01))对氮营养盐的生理响应.结果表明,两种赤潮生物的最大比增长率均随着培养液中硝酸盐浓度的升高(20～80 μmol/L)而增加,且硝酸盐培养下的最大比增长速率高于同浓度的氨盐,表明在高浓度下,硝酸盐是适宜的氮源.东海原甲藻硝酸还原酶活力(1.14～5.20 fmol/(cell·L·min))与硝酸盐吸收速率呈正相关,链状亚历山大藻中则未检测到硝酸还原酶活力;两种赤潮生物谷酰氨合成酶活力对硝酸盐和氨盐的响应存在差异,链状亚历山大藻谷氨酰胺合成酶活力(10.93～30.97 pmol/(cell·L·min))要远高于东海原甲藻(0.34～0.95 pmol/(cell·L·min)).两种赤潮生物对硝酸盐和氨盐的不同生理响应可能是引起赤潮期间种群更替的一个重要原因.
比較研究瞭硝痠鹽和氨鹽培養下,兩種甲藻(東海原甲藻(Prorocentrum donghaiense Lu)和鏈狀亞歷山大藻(Alexandrium catenella DH01))對氮營養鹽的生理響應.結果錶明,兩種赤潮生物的最大比增長率均隨著培養液中硝痠鹽濃度的升高(20～80 μmol/L)而增加,且硝痠鹽培養下的最大比增長速率高于同濃度的氨鹽,錶明在高濃度下,硝痠鹽是適宜的氮源.東海原甲藻硝痠還原酶活力(1.14～5.20 fmol/(cell·L·min))與硝痠鹽吸收速率呈正相關,鏈狀亞歷山大藻中則未檢測到硝痠還原酶活力;兩種赤潮生物穀酰氨閤成酶活力對硝痠鹽和氨鹽的響應存在差異,鏈狀亞歷山大藻穀氨酰胺閤成酶活力(10.93～30.97 pmol/(cell·L·min))要遠高于東海原甲藻(0.34～0.95 pmol/(cell·L·min)).兩種赤潮生物對硝痠鹽和氨鹽的不同生理響應可能是引起赤潮期間種群更替的一箇重要原因.
비교연구료초산염화안염배양하,량충갑조(동해원갑조(Prorocentrum donghaiense Lu)화련상아력산대조(Alexandrium catenella DH01))대담영양염적생리향응.결과표명,량충적조생물적최대비증장솔균수착배양액중초산염농도적승고(20～80 μmol/L)이증가,차초산염배양하적최대비증장속솔고우동농도적안염,표명재고농도하,초산염시괄의적담원.동해원갑조초산환원매활력(1.14～5.20 fmol/(cell·L·min))여초산염흡수속솔정정상관,련상아력산대조중칙미검측도초산환원매활력;량충적조생물곡선안합성매활력대초산염화안염적향응존재차이,련상아력산대조곡안선알합성매활력(10.93～30.97 pmol/(cell·L·min))요원고우동해원갑조(0.34～0.95 pmol/(cell·L·min)).량충적조생물대초산염화안염적불동생리향응가능시인기적조기간충군경체적일개중요원인.
This study investigated physiological response of Prorocentrum donghaiense Lu and Alexandrium catenella DH01 to nitrate and ammonium.The results showed that the growth rates of P.donghaiense and A.catenella increased with the nitrate concentrations(20～80 μmol/L)of the culture medium,and both of them presented higher growth rate in nitrate than in ammonium.In high nutrient concentration,nitrate was regarded as the optimal nitrogen source.The nitrate reductase activity(1.14～5.20 fmol/(cell·L·min))of P.donghaiense exhibited a positive relationship with the nitrate concentrations of the culture medium,while the NR activity of A.catenella could not be detected.The responses of glutamine synthetase in P.donghaiense and A.catenella to nitrate and ammonium was different,and glutamine synthetase activity(10.93～30.97 pmol/(cell·L·min))of A.catenella was higher than that of P.donghaiense(0.34～0.95 pmol/(cell·L·min)).The different physiological responses to nitrate and ammonium between P.donghaiense and A.catenella might be one of the important factors to result in population succession during the process of harmful algal blooms.
