CAJ | 학술논문

采用正交试验法研究超临界CO_2萃取法提取小花假泽兰精油的提取条件,采用GC-MS法分析测定精油的化学成分,并初步测定精油的抑菌效果.结果表明:超临界CO_2萃取小花假泽兰精油的较佳工艺条件为:采用先静态后动态的萃取方式,静态萃取时间20 min,动态萃取条件为萃取温度55℃,萃取压力35 Mpa,CO_2体积为40 mL/g,CO_2 流速为0.5～1.0 mL/min;从超临界CO_2萃取的小花假泽兰精油中鉴定了64种成分,占精油总量的79.80%,主要有萜类、醇类、脂肪酸、酯类、甾体类等;生物测定结果表明,超临界CO_2萃取物对小麦赤霉病菌和小麦纹枯病菌菌丝生长的EC_(50)分别为119.55 mg/L和78.27 mg/L.果.结果表明:超临界CO_2萃取小花假泽兰精油的较佳工艺条件为:采用先静态后动态的萃取方式,静态萃取时间20 min,动态萃取条件为萃取温度55℃,萃取压力35 MPa,CO_2体积为40 mL/g,CO_2 流速为0.5～1.0 mL/min;从超临界CO2萃取的小花假泽兰精油中鉴定了64种成分,占精油总量的79.80%,主要有萜类、醇类、脂肪酸、酯类、甾体类等;生物测定结果表,超临界CO_2萃取物对
채용정교시험법연구초림계CO_2췌취법제취소화가택란정유적제취조건,채용GC-MS법분석측정정유적화학성분,병초보측정정유적억균효과.결과표명:초림계CO_2췌취소화가택란정유적교가공예조건위:채용선정태후동태적췌취방식,정태췌취시간20 min,동태췌취조건위췌취온도55℃,췌취압력35 Mpa,CO_2체적위40 mL/g,CO_2 류속위0.5～1.0 mL/min;종초림계CO_2췌취적소화가택란정유중감정료64충성분,점정유총량적79.80%,주요유첩류、순류、지방산、지류、치체류등;생물측정결과표명,초림계CO_2췌취물대소맥적매병균화소맥문고병균균사생장적EC_(50)분별위119.55 mg/L화78.27 mg/L.과.결과표명:초림계CO_2췌취소화가택란정유적교가공예조건위:채용선정태후동태적췌취방식,정태췌취시간20 min,동태췌취조건위췌취온도55℃,췌취압력35 MPa,CO_2체적위40 mL/g,CO_2 류속위0.5～1.0 mL/min;종초림계CO2췌취적소화가택란정유중감정료64충성분,점정유총량적79.80%,주요유첩류、순류、지방산、지류、치체류등;생물측정결과표,초림계CO_2췌취물대
The paper introduced that the essential oils was obtained from Mikania micrantha H.B.K.by supercritical CO_2 extraction method.The optimum condition was determined by the orthogonal experiments.The extraction was analyzed by GC/MS,and the fungicidal activities were preliminarily tested.The results showed that the optimal conditions for the improved supercritical CO_2 extraction of essential oil were determined as follows:Static extraction time 20 min;Dynamic extraction conditions:extraction temperature 55℃,extraction press 35 MPa,volume of CO_2 40 mL/g,CO_2 flow rate 0.5～1.0 mL/min.Under these conditions,the essential oils contained 64 kinds of compounds and 79.80% of its main components were terpenoids,alcohols,fatty acids,esters and steroidal compounds.The test of the inhibition rate against the mycelial growth showed that the essential oil had strong inhibitory on growth of Fusarium graminearum and Rhizoctonia cerealis,which EC_(50) value was 119.55 mg/L and 78.27 mg/L.