CAJ | 학술논문

本文利用具有特殊的电子结构的类金刚石(DLC)薄膜来研究材料表面电子结构对人脐静脉内皮细胞(HUVEC)附着性能的影响.采用直流电弧脉冲等离子体沉积法制备DLC薄膜,通过改变靶室气体种类和气体压强来控制DLC薄膜中sp2键的含量,并通过Raman光谱分析法进行测定.采用DAPI免疫荧光染色法对细胞附着形态进行观察,并利用Image J对随机记录的多个视场的所有细胞进行了细胞附着形态的统计学分析.实验结果表明:在充入Ar气制备的DLC薄膜表面,内皮细胞的附着性能随着sp2键含量的升高而变差,充入Ar/C2H2混合气体制备的DLC薄膜因所有细胞附着形态均很差而未体现出随sp2键含量而变化的明显趋势.
본문이용구유특수적전자결구적류금강석(DLC)박막래연구재료표면전자결구대인제정맥내피세포(HUVEC)부착성능적영향.채용직류전호맥충등리자체침적법제비DLC박막,통과개변파실기체충류화기체압강래공제DLC박막중sp2건적함량,병통과Raman광보분석법진행측정.채용DAPI면역형광염색법대세포부착형태진행관찰,병이용Image J대수궤기록적다개시장적소유세포진행료세포부착형태적통계학분석.실험결과표명:재충입Ar기제비적DLC박막표면,내피세포적부착성능수착sp2건함량적승고이변차,충입Ar/C2H2혼합기체제비적DLC박막인소유세포부착형태균흔차이미체현출수sp2건함량이변화적명현추세.
In this paper,Diamond-Liked Carbon(DLC)Films with different sp2 content were used to investigate the influence of electric property on the adhesion behavior of HUVEC(human umbilical vein endothelial cells).The DLC films were prepared by means of Pulsed Arc Plasma Sputtering Deposition.The relative content of sp2 bond of DLC films was adjusted by alternate the species and flow rate of the chamber gas,and was detected by Raman spectroscopy.The adhesion configuration of HUVEC was observed by immunofluorescent staining method,and Image J software was used to statistically analyzing the configuration of the adhered cells.The results show that:on the DLC films that are prepared in Ar atmosphere.the adhesion state of HUVEC tencls to be worse as the content of sp2 bond increasing;while on the films that are prepared in Ar/C2 H2 atmosphere,the adhesion state of HUVEC is so bad that no significant tendence was found.