CAJ | 학술논문

为了揭示河北省绿子叶黑豆种质资源的遗传多样性,为其研究利用提供理论根据,以46份原产河北省的绿子叶黑豆种质资源为试验材料,对其基于表型性状及ISSR标记鉴定结果进行了聚类分析,结果表明:7个ISSR引物共检测出60个等位变异,平均每个位点有8.6个等位变异,变幅为5～17个;ISSR引物的多态性信息量PIC变幅为0.721～0.927,平均0.820;利用表型性状和ISSR标记数据进行品种间遗传多样性分析,遗传相似系数变化范围分别为0.07～0.53和0.43～1.00,平均为0.284和0.704.遗传相似性变幅较大,河北省不同绿子叶黑豆品种间存在着丰富的遗传多样性.聚类结果显示,类群与品种来源地有关.
위료게시하북성록자협흑두충질자원적유전다양성,위기연구이용제공이론근거,이46빈원산하북성적록자협흑두충질자원위시험재료,대기기우표형성상급ISSR표기감정결과진행료취류분석,결과표명:7개ISSR인물공검측출60개등위변이,평균매개위점유8.6개등위변이,변폭위5～17개;ISSR인물적다태성신식량PIC변폭위0.721～0.927,평균0.820;이용표형성상화ISSR표기수거진행품충간유전다양성분석,유전상사계수변화범위분별위0.07～0.53화0.43～1.00,평균위0.284화0.704.유전상사성변폭교대,하북성불동록자협흑두품충간존재착봉부적유전다양성.취류결과현시,류군여품충래원지유관.
The genetie diversity of green-cotyledon black soybean cuhivars native to Hebei province was evaluated based on ISSR and agronomic characters.The results indicated that 60 alleles of 7 ISSR markers were detected among 46 cultivars with an average of 8.57 alleles per ISSR marker,and the number of alleles per primer ranged from 5 to 17.The value of allelic polymorphism information content (PIC) ranged from 0.721 to 0.927,on the average of 0.82 per primer.The value of genetic similarity (GS) indices of 46 cultivars based on the 14 agronomic traits and ISSR data varied from 0.067 to 0.533 and 0.43 to 1.0 respectively,with an average of 0.284 and 0.704,indicated that the genetic diversity among green-coty ledon black soybean cultivars from Hebei was abundant.The clustering results showed that the groups could reflect the origin of the cuhivars.