CAJ | 학술논문

UV-B辐射作为一种重要的环境信号影响着植物的生长与发育,它能够调控气孔运动和诱导乙烯产生.该试验利用乙烯生物合成抑制剂和乙烯受体抑制剂处理蚕豆叶片表皮条,结合气孔开度分析和乙烯释放量测定,研究乙烯在UV-B辐射调控表皮条气孔运动中的作用.结果发现,将蚕豆叶片表皮条置于0.8 W·m-2的UV-B辐射下1～4 h,乙烯生成和气孔关闭均被显著诱导,且乙烯释放峰先于气孔关闭的起始;乙烯生物合成抑制剂和乙烯受体抑制剂处理均能显著逆转UV-B辐射诱导的气孔关闭;外源乙烯处理也能模拟UV-B辐射的效应诱导可见光下蚕豆表皮条的气孔关闭.可见,乙烯介导了UV-B辐射诱导的蚕豆气孔关闭.
UV-B복사작위일충중요적배경신호영향착식물적생장여발육,타능구조공기공운동화유도을희산생.해시험이용을희생물합성억제제화을희수체억제제처리잠두협편표피조,결합기공개도분석화을희석방량측정,연구을희재UV-B복사조공표피조기공운동중적작용.결과발현,장잠두협편표피조치우0.8 W·m-2적UV-B복사하1～4 h,을희생성화기공관폐균피현저유도,차을희석방봉선우기공관폐적기시;을희생물합성억제제화을희수체억제제처리균능현저역전UV-B복사유도적기공관폐;외원을희처리야능모의UV-B복사적효응유도가견광하잠두표피조적기공관폐.가견,을희개도료UV-B복사유도적잠두기공관폐.
Ultraviolet B (UV-B) irradiation is an important environmental signal for plant growth and development.Despite the well-known that UV-B can regulate stomatal movement and induce ethylene synthesis,the physiological role of ethylene in UV-B-regulated stomatal movement is largely unknown.Here,by epidermal strip bioassay and assays of ethylene production,the role of ethylene in UV-B-regulated stomatal movement was investigated in epidermal strips of broad bean (Vicia faba L.) plants.When epidermal strips were exposed to 0.8 were significantly induced,and ethylene evolution peak preceded the initiation of stomatal closure.Furthermore,the UV-B-induced stomatal closure was largely reversed by interfering ethylene biosynthesis as well as ethylene perception,while exogenous ethylene induced stomatal closure indeed.These results clearly show that ethylene mediates the UV-B-induced stomatal closure in broad bean plants.