国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2011年
4期
452-455
,共4页
膀胱肿瘤
膀胱腫瘤
방광종류
Bladder Neoplasms
目的 探讨PTEN mRNA和Survivin mRNA在膀胱移行细胞癌中的表达及其与病理分级、临床分期和复发的关系.方法 采用SYBR Green Ⅱ实时荧光定量逆转录聚合酶链反应试验方法检测43例膀胱癌组织及9例膀胱正常组织中PTEN mRNA和Survivin mRNA的表达情况.结果 膀胱正常组织中PTEN mRNA的表达高于膀胱癌组织,Survivin mRNA表达高于膀胱癌组织,两者比较差异有统计学意义(P<0.05);PTEN mRNA和Survivin mRNA在膀胱癌低级别和高级别、表浅型和浸润型、初发和复发中的表达比较差异均有统计学意义(p<0.05).PTEN mRNA和Survivin mRNA在膀胱癌中的表达成负相关(r=0.972,p<0.05).结论 PTEN和Survivin参与了膀胱癌的发生发展过程,对膀胱癌的诊断、判断病理分级.临床分期和预后具有重要临床意义,膀胱癌中PTEN和Survivin两者mBNA的表达呈负相关,二者表达的失调可能是BTCC的发生发展的机制之一.
目的 探討PTEN mRNA和Survivin mRNA在膀胱移行細胞癌中的錶達及其與病理分級、臨床分期和複髮的關繫.方法 採用SYBR Green Ⅱ實時熒光定量逆轉錄聚閤酶鏈反應試驗方法檢測43例膀胱癌組織及9例膀胱正常組織中PTEN mRNA和Survivin mRNA的錶達情況.結果 膀胱正常組織中PTEN mRNA的錶達高于膀胱癌組織,Survivin mRNA錶達高于膀胱癌組織,兩者比較差異有統計學意義(P<0.05);PTEN mRNA和Survivin mRNA在膀胱癌低級彆和高級彆、錶淺型和浸潤型、初髮和複髮中的錶達比較差異均有統計學意義(p<0.05).PTEN mRNA和Survivin mRNA在膀胱癌中的錶達成負相關(r=0.972,p<0.05).結論 PTEN和Survivin參與瞭膀胱癌的髮生髮展過程,對膀胱癌的診斷、判斷病理分級.臨床分期和預後具有重要臨床意義,膀胱癌中PTEN和Survivin兩者mBNA的錶達呈負相關,二者錶達的失調可能是BTCC的髮生髮展的機製之一.
목적 탐토PTEN mRNA화Survivin mRNA재방광이행세포암중적표체급기여병리분급、림상분기화복발적관계.방법 채용SYBR Green Ⅱ실시형광정량역전록취합매련반응시험방법검측43례방광암조직급9례방광정상조직중PTEN mRNA화Survivin mRNA적표체정황.결과 방광정상조직중PTEN mRNA적표체고우방광암조직,Survivin mRNA표체고우방광암조직,량자비교차이유통계학의의(P<0.05);PTEN mRNA화Survivin mRNA재방광암저급별화고급별、표천형화침윤형、초발화복발중적표체비교차이균유통계학의의(p<0.05).PTEN mRNA화Survivin mRNA재방광암중적표체성부상관(r=0.972,p<0.05).결론 PTEN화Survivin삼여료방광암적발생발전과정,대방광암적진단、판단병리분급.림상분기화예후구유중요림상의의,방광암중PTEN화Survivin량자mBNA적표체정부상관,이자표체적실조가능시BTCC적발생발전적궤제지일.
Objectives To study expression of PTEN mRNA 和 Survivin mRNA in BTCC and its relationship between BTCC and normal tissue 、clinical stage、pathology grade and recurrence. Methods The SYBR Green Ⅱ real time quantitative RT-PCR was used to evaluated the expression of PTEN mRNA 和 Survivin mRNA in 43 cases of transitional cell carcinoma of urinary bladder and 9 normal cases. Results The expression of PTEN mRNA in normol tissue is higher than that in BTCC,and The expression of Survivin mRNA in normol tissue is lower than that in BTCC There were statistical significance between normol tissue and BTCC。 There were also statistical significance between the grade ( p <0.05) , the clinical stage (p <0.05) , and primary and recurrence (p < 0.05).There was a negative correlation between the expression of PTEN mRNA 和 Survivin mRNA in BTCC(r= -0.972, p <0.05).Conclusions PTEN mRNA 和 Survivin mRNA may be implicated in the patnogenesis of BTCC,and they play role in diagnosing BTCC,judging pathological grade and clinical stage and have important clinical significance. The
imbalance expression of them may be a reason of the original and developong mechanism of BTCC.
