CAJ | 학술논문

目的研究maspin蛋白及p53蛋白在宫颈鳞癌及宫颈上皮内瘤样变组织中的表达及与高危型人乳头瘤病毒(HPV)感染的相关性.方法应用免疫组化SP法检测maspin蛋白和p53蛋白在宫颈鳞癌、宫颈上皮内瘤样变、正常宫颈3种组织中的表达.分析maspin蛋白表达与宫颈鳞癌各临床病理参数之间的关系,以及宫颈鳞癌组织中maspin蛋白与p53蛋白表达的相关性.HPV检测采用PCR扩增-导流杂交的方法进行分型检测,并将高危性感染率与maspin表达率进行相关性分析.结果 (1)从正常宫颈上皮组织→宫颈上皮内瘤样变→宫颈鳞癌maspin蛋白表达逐渐下调,三者比较差异有统计学意义(P<0.05);(2)临床分期越高,分化程度越低的宫颈鳞癌组织中,maspin阳性表达越少.有淋巴结转移的宫颈鳞癌中,maspin阳性率低于无淋巴结转移者,2者比较差异有统计学意义(P<0.05);(3)maspin蛋白与p53蛋白在宫颈鳞癌组织中的表达呈负相关(r_s=-0.399,P<0.01);(4)maspin蛋白的表达与高危型HPV感染呈负相关(r_s=-0.294,P<0.01).结论 maspin蛋白在宫颈鳞癌的发生发展过程中起重要的抑制作用,可作为判断宫颈鳞癌患者病情进展及预后的指标之一.maspin蛋白在高危性HPV感染后可能受p53等因子的调控表达下调,可用于宫颈癌的基因治疗研究.
목적 연구maspin단백급p53단백재궁경린암급궁경상피내류양변조직중적표체급여고위형인유두류병독(HPV)감염적상관성.방법 응용면역조화SP법검측maspin단백화p53단백재궁경린암、궁경상피내류양변、정상궁경3충조직중적표체.분석maspin단백표체여궁경린암각림상병리삼수지간적관계,이급궁경린암조직중maspin단백여p53단백표체적상관성.HPV검측채용PCR확증-도류잡교적방법진행분형검측,병장고위성감염솔여maspin표체솔진행상관성분석.결과 (1)종정상궁경상피조직→궁경상피내류양변→궁경린암maspin단백표체축점하조,삼자비교차이유통계학의의(P<0.05);(2)림상분기월고,분화정도월저적궁경린암조직중,maspin양성표체월소.유림파결전이적궁경린암중,maspin양성솔저우무림파결전이자,2자비교차이유통계학의의(P<0.05);(3)maspin단백여p53단백재궁경린암조직중적표체정부상관(r_s=-0.399,P<0.01);(4)maspin단백적표체여고위형HPV감염정부상관(r_s=-0.294,P<0.01).결론 maspin단백재궁경린암적발생발전과정중기중요적억제작용,가작위판단궁경린암환자병정진전급예후적지표지일.maspin단백재고위성HPV감염후가능수p53등인자적조공표체하조,가용우궁경암적기인치료연구.
Objective To investigate expression of maspin protein in cervical squamous carcinoma (CSC) and evaluate its possible role in tumorigenesis and progression of cervical carcinoma, and to further investigate its relationship between p53 expression and HPV infection. Methods Levels of maspin protein and p53 protein in cervical squamous carcinoma, cervical intraepithelial neoplasia and normal uterine neck were detected by immunohistochemistry. Analyses were made concerning possible relationship between the expression of 2 proteins and clinicopathologic indices and correlation between maspin in CSC and p53 expression. HPV detection was made with PCR amplification and hybridization. Results (1) Expression of maspin in squamous cell carcinoma of the cervix gradually down-regulated, as seen from normal cervical tissue to cervical intraepitbelial neoplasia to squamous cell carcinoma of the cervix with statistical significance (P < 0.05). The higher the clinical stages and the lower the cell differentiation, less expression of maspin there was. In cervical squamous carcinoma with lymphatic metastasis, positive expression rate of maspin was lower than that without lymphatic metastasis. And significant differences could be noted when comparisons were made between the two (P <0.05). Negative correlation could be seen between expressions of maspin and p53 in cervical squamous carcinoma(r_s = -0.399, P < 0.05). (2) Negative correlation was shown between expressions of maspin and the high-risk HPV infection(r_s =-0.294 ,P < 0.05). Conclusions Maspin might play a strong inhibitory role in the genesis and development of squamous cell carcinoma of the cervix. Abnormal expression of maspin might