CAJ | 학술논문

目的运用惊跳反射弱刺激抑制(PPI)实验方法对一次单纯性脑震荡(PCC)与多重性脑震荡(MCC)大鼠的感觉门控变化进行研究,以探讨认知损伤的积累效应.方法用金属单摆闭合性脑损伤打击装置复制PCC和MCC大鼠,另设正常对照组,每组动物10只.自损伤前3d至伤后28d,检测大鼠在惊刺激诱发下的惊吓反射波幅P值、三种不同前置弱刺激惊吓反射波幅值PP67,PP69和PP73值及惊跳反射弱刺激抑制值PPI67,PPI69和PP173的变化情况.结果 PCC后24h内P值及上述3个PP值开始降低(P＜0.05),至损伤16d后接近或恢复正常；MCC后24h内P值及上述3个PP值开始显著降低(P＜0.05)、且比PCC组更加明显,至伤后28d仍未恢复；PCC和MCC组的PPI67,PPI69和PPI73值在部分实验测试点明显高于正常.大鼠脑震荡次数越多,惊吓刺激反射P值和PP值损害性减弱越明显,而惊跳反射弱刺激抑制强度PPI值仅表现出一定程度的加强.结论大鼠脑震荡后,不论PCC还是MCC,其感觉门控作用均出现一定程度的增强,惊吓刺激反射和前置弱刺激惊吓反射的减弱变化则表现出明显的损伤性累加效应.
목적 운용량도반사약자격억제(PPI)실험방법대일차단순성뇌진탕(PCC)여다중성뇌진탕(MCC)대서적감각문공변화진행연구,이탐토인지손상적적루효응.방법 용금속단파폐합성뇌손상타격장치복제PCC화MCC대서,령설정상대조조,매조동물10지.자손상전3d지상후28d,검측대서재량자격유발하적량하반사파폭P치、삼충불동전치약자격량하반사파폭치PP67,PP69화PP73치급량도반사약자격억제치PPI67,PPI69화PP173적변화정황.결과 PCC후24h내P치급상술3개PP치개시강저(P＜0.05),지손상16d후접근혹회복정상；MCC후24h내P치급상술3개PP치개시현저강저(P＜0.05)、차비PCC조경가명현,지상후28d잉미회복；PCC화MCC조적PPI67,PPI69화PPI73치재부분실험측시점명현고우정상.대서뇌진탕차수월다,량하자격반사P치화PP치손해성감약월명현,이량도반사약자격억제강도PPI치부표현출일정정도적가강.결론 대서뇌진탕후,불론PCC환시MCC,기감각문공작용균출현일정정도적증강,량하자격반사화전치약자격량하반사적감약변화칙표현출명현적손상성루가효응.
Objective To observe the prepulse inhibition(PPI) ot the startle reflex of pure cerebral concussion (PCC) suffered from one concussion and multiple cerebral concussion (MCC) suffered from three concussions in rats,and to explore accumulate effect upon cognitive dysfunction of MCC.Methods A metallic pendulum striker device for closed head injury was employed to duplicate PCC and MCC models in Stragu-Dawley rats.The MCC rats were hit three times on rats'head and it is interval 24 hours for every hit.According to the criteria of cerebral concussion,the investigated animals were divided into PCC group and MCC group at freedom.One control group was used.Each group included 10 animals.Each experience mental animal was tested from 3 days pre-injury to 28 days post-concussion.Startle reflex amplitude (for P values),pre-stimulation induced reflex amplitude on three standard stimulations,that was,67dB,69dB and 73dB (for PP67,PP69 and PP73 values) and prepulse inhibition of the startle reflex (PPI) were collected.Results The P values and three PP values in the first three days of pre-replication experiment,there was no statistical significance in each group.However the P values and PP67,PP69 and PP73 values declined until to the 16th day after injury (P＜0.05),then recovered in PCC group.The P value and PP67,PP69 and PP73 values changes of MCC group declined and not recovered until to test end (P＜0.05 ) and they were more lower than PCC.The three PPI values were a little bit increase in both groups,there were statistics significance at some test points (P＜0.05) compared with control.Conclusion The startle reflex amplitude and pre-stimulation induced reflex amplitude weaken after cerebral concussion and there is damaging accumulate effect to injury times,the PPI is enlanced by cerebral concussion.