中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
7期
726-729
,共4页
龚成%罗明%陈萌%张铁钢%张合润%王玉梅%李仁清%董梅%陈维新%陈丽娟
龔成%囉明%陳萌%張鐵鋼%張閤潤%王玉梅%李仁清%董梅%陳維新%陳麗娟
공성%라명%진맹%장철강%장합윤%왕옥매%리인청%동매%진유신%진려연
脊髓灰质炎病毒%脊髓灰质炎野病毒%实时荧光RT-PCR
脊髓灰質炎病毒%脊髓灰質炎野病毒%實時熒光RT-PCR
척수회질염병독%척수회질염야병독%실시형광RT-PCR
Poliovirus%Poliomyelitis,wild poliovirus%Real time RT-PCR
目的 评价和探讨Ⅰ型脊髓灰质炎(脊灰)野病毒快速检测策略.方法 采集671名来自脊灰野病毒流行疫区的在京学生粪便标本,采用一组实时荧光RT-PCR代替标准方法分别进行肠道病毒通用型检测、脊灰病毒分型检测、Ⅰ型脊灰疫苗株和野毒株鉴别检测.并利用Ⅰ、Ⅱ和Ⅲ型脊灰病毒标准株(Sabin株)和Ⅰ型野毒株对实时荧光RT-PCR和标准方法的灵敏度进行比较和评价.结果 (1)新脊灰病毒检测流程(肠道病毒通用型检测+脊灰病毒分型检测+脊灰野毒鉴别检测)检出非脊灰肠道病毒33例；脊灰病毒疫苗株16例,其中Ⅰ型5例、Ⅱ型1例、Ⅲ型3例、Ⅰ+Ⅱ型1例、Ⅰ+Ⅲ型4例、Ⅰ+Ⅱ+Ⅲ型2例；脊灰病毒Ⅰ型野毒株3例.(2)3种实时荧光RT-PCR检测脊灰病毒比标准方法敏感1～ 100倍.其中肠道通用型检测和脊灰病毒分型检测2种方法对Ⅱ型脊灰病毒疫苗株的检测,比标准方法敏感100倍；脊灰病毒分型检测能准确区分Ⅰ、Ⅱ和Ⅲ型脊灰疫苗株,脊灰野毒株鉴别检测对Ⅰ型疫苗株的检测比标准方法敏感10倍；3种实时荧光RT-PCR均能有效检测出Ⅰ型脊灰野毒株,比标准方法敏感10倍.结论 新的脊灰病毒检测流程,可大幅缩短检测时间,提高检测通量,且灵敏度高于标准方法,适合于脊灰应急检测应对突发疫情.
目的 評價和探討Ⅰ型脊髓灰質炎(脊灰)野病毒快速檢測策略.方法 採集671名來自脊灰野病毒流行疫區的在京學生糞便標本,採用一組實時熒光RT-PCR代替標準方法分彆進行腸道病毒通用型檢測、脊灰病毒分型檢測、Ⅰ型脊灰疫苗株和野毒株鑒彆檢測.併利用Ⅰ、Ⅱ和Ⅲ型脊灰病毒標準株(Sabin株)和Ⅰ型野毒株對實時熒光RT-PCR和標準方法的靈敏度進行比較和評價.結果 (1)新脊灰病毒檢測流程(腸道病毒通用型檢測+脊灰病毒分型檢測+脊灰野毒鑒彆檢測)檢齣非脊灰腸道病毒33例；脊灰病毒疫苗株16例,其中Ⅰ型5例、Ⅱ型1例、Ⅲ型3例、Ⅰ+Ⅱ型1例、Ⅰ+Ⅲ型4例、Ⅰ+Ⅱ+Ⅲ型2例；脊灰病毒Ⅰ型野毒株3例.(2)3種實時熒光RT-PCR檢測脊灰病毒比標準方法敏感1～ 100倍.其中腸道通用型檢測和脊灰病毒分型檢測2種方法對Ⅱ型脊灰病毒疫苗株的檢測,比標準方法敏感100倍；脊灰病毒分型檢測能準確區分Ⅰ、Ⅱ和Ⅲ型脊灰疫苗株,脊灰野毒株鑒彆檢測對Ⅰ型疫苗株的檢測比標準方法敏感10倍；3種實時熒光RT-PCR均能有效檢測齣Ⅰ型脊灰野毒株,比標準方法敏感10倍.結論 新的脊灰病毒檢測流程,可大幅縮短檢測時間,提高檢測通量,且靈敏度高于標準方法,適閤于脊灰應急檢測應對突髮疫情.
목적 평개화탐토Ⅰ형척수회질염(척회)야병독쾌속검측책략.방법 채집671명래자척회야병독류행역구적재경학생분편표본,채용일조실시형광RT-PCR대체표준방법분별진행장도병독통용형검측、척회병독분형검측、Ⅰ형척회역묘주화야독주감별검측.병이용Ⅰ、Ⅱ화Ⅲ형척회병독표준주(Sabin주)화Ⅰ형야독주대실시형광RT-PCR화표준방법적령민도진행비교화평개.결과 (1)신척회병독검측류정(장도병독통용형검측+척회병독분형검측+척회야독감별검측)검출비척회장도병독33례；척회병독역묘주16례,기중Ⅰ형5례、Ⅱ형1례、Ⅲ형3례、Ⅰ+Ⅱ형1례、Ⅰ+Ⅲ형4례、Ⅰ+Ⅱ+Ⅲ형2례；척회병독Ⅰ형야독주3례.(2)3충실시형광RT-PCR검측척회병독비표준방법민감1～ 100배.기중장도통용형검측화척회병독분형검측2충방법대Ⅱ형척회병독역묘주적검측,비표준방법민감100배；척회병독분형검측능준학구분Ⅰ、Ⅱ화Ⅲ형척회역묘주,척회야독주감별검측대Ⅰ형역묘주적검측비표준방법민감10배；3충실시형광RT-PCR균능유효검측출Ⅰ형척회야독주,비표준방법민감10배.결론 신적척회병독검측류정,가대폭축단검측시간,제고검측통량,차령민도고우표준방법,괄합우척회응급검측응대돌발역정.
Objective To explore the Fast Testing Sstrategy (FTS) for wild poliovirus Ⅰ (WP1).Methods Epidemiological investigations were carried out on 671 students from WP1 epidemic areas in China.A set of real time RT-PCR assays,including panenterovirus testings (PE) assay,poliovirus serotypings(PS) assay and the assay distinguishing wild strain from vaccine strain of poliovirus Ⅰ (DWV) were introduced into the screening program for WPV1 to replace the conventional RT-PCR,recommended by the China National Polio Laboratory (GNPL).Additionally,sensitivities of all the assays were assessed by poliovirus type Ⅰ to Ⅲ (Sabin stain) and the isolated WPV I.Results ( 1 ) 33 non-poliovirus enterovirus (NPEV) cases were detected,with 16 polio vaccinerelated cases including 5 polio Ⅰ,1 polio Ⅱ,3 polio Ⅲ,1 polio Ⅰ +Ⅱ,4 polio Ⅰ + Ⅲ and 2 polio Ⅰ + Ⅱ + Ⅲ.Three WPV 1 cases were also detected in this study and confirmed by GNPL.(2) For polio virus vaccine strain,sensitivities of the set of real time RT-PCR assays ranged from 1 to 100 times than that of the in-housc RT-PCR assay.The sensitivities of PE and PS assays for the detection of polio Ⅱ were 100 times than that of the RT-PCR assay and the sensitivity of DWV assay used for the detection of polio Ⅰ were 10 times than that of the RT-PCR assay.For WPV1,the sensitivity of three real time RT-PCR was 10 times higher than that of the RT-PCR assay.Conclusion The novel FTS for WPV I suggested by this study would include PE,PS and DWV.It not only could greatly shorten the testing time but also more sensitive than the RT-PCR and suited for emergency detection for WPV1.
