中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2011年
4期
416-419
,共4页
徐辉辉%汪黎%隋丽%关华%王豫%刘晓丹%张士猛%徐勤枝%王潇%周平坤
徐輝輝%汪黎%隋麗%關華%王豫%劉曉丹%張士猛%徐勤枝%王瀟%週平坤
서휘휘%왕려%수려%관화%왕예%류효단%장사맹%서근지%왕소%주평곤
香兰素衍生物%DNA损伤%辐射防护%γ射线%重离子%质子
香蘭素衍生物%DNA損傷%輻射防護%γ射線%重離子%質子
향란소연생물%DNA손상%복사방호%γ사선%중리자%질자
Vanillin derivative%DNA damage%Radiation protection%γ-rays%Heavy ion%Proton
目的 评价不同LET辐射致DNA损伤以及药物的防护作用.方法 分别以60Co γ射线、质子束、7Li重离子束照射溶液状态超螺旋构象质粒DNA,通过琼脂糖凝胶电泳分析DNA分子构象变化,检测DNA损伤程度及VND3207的防护作用.结果 质子和7Li重离子体外所致质粒DNA损伤明显比γ射线严重.药物VND3207能够有效减轻所观察的3种不同LET辐射对质粒DNA的损伤,加药组与不加药组相比,质粒DNA开环构象显著减少,保护效果随着药物浓度的增加更加明显.200 μmol/L浓度对50 Gy γ射线、质粒和7Li重离子辐照质粒DNA损伤的保护效果(质粒超螺旋构象百分比)分别为85.3%(t=3.70,P=0.033)、73.3%(t=10.58,P=0.017)和80.4%(t=8.57,P=0.008).可见VND3207对重离子辐射损伤的保护效应尤为显著,明显优于质子辐照损伤.结论 药物VND3207对辐射DNA损伤具有较好的保护作用,特别是对γ射线和重离子辐射损伤的保护作用更为明显.
目的 評價不同LET輻射緻DNA損傷以及藥物的防護作用.方法 分彆以60Co γ射線、質子束、7Li重離子束照射溶液狀態超螺鏇構象質粒DNA,通過瓊脂糖凝膠電泳分析DNA分子構象變化,檢測DNA損傷程度及VND3207的防護作用.結果 質子和7Li重離子體外所緻質粒DNA損傷明顯比γ射線嚴重.藥物VND3207能夠有效減輕所觀察的3種不同LET輻射對質粒DNA的損傷,加藥組與不加藥組相比,質粒DNA開環構象顯著減少,保護效果隨著藥物濃度的增加更加明顯.200 μmol/L濃度對50 Gy γ射線、質粒和7Li重離子輻照質粒DNA損傷的保護效果(質粒超螺鏇構象百分比)分彆為85.3%(t=3.70,P=0.033)、73.3%(t=10.58,P=0.017)和80.4%(t=8.57,P=0.008).可見VND3207對重離子輻射損傷的保護效應尤為顯著,明顯優于質子輻照損傷.結論 藥物VND3207對輻射DNA損傷具有較好的保護作用,特彆是對γ射線和重離子輻射損傷的保護作用更為明顯.
목적 평개불동LET복사치DNA손상이급약물적방호작용.방법 분별이60Co γ사선、질자속、7Li중리자속조사용액상태초라선구상질립DNA,통과경지당응효전영분석DNA분자구상변화,검측DNA손상정도급VND3207적방호작용.결과 질자화7Li중리자체외소치질립DNA손상명현비γ사선엄중.약물VND3207능구유효감경소관찰적3충불동LET복사대질립DNA적손상,가약조여불가약조상비,질립DNA개배구상현저감소,보호효과수착약물농도적증가경가명현.200 μmol/L농도대50 Gy γ사선、질립화7Li중리자복조질립DNA손상적보호효과(질립초라선구상백분비)분별위85.3%(t=3.70,P=0.033)、73.3%(t=10.58,P=0.017)화80.4%(t=8.57,P=0.008).가견VND3207대중리자복사손상적보호효응우위현저,명현우우질자복조손상.결론 약물VND3207대복사DNA손상구유교호적보호작용,특별시대γ사선화중리자복사손상적보호작용경위명현.
Objective To evaluate the radioprotective effect of vanillin derivative VND3207 on DNA damage induced by different LET ionizing radiation.Methods The plasmid DNA in liquid was irradiated by 60Co γ-rays, proton or 7Li heavy ion with or without VND3207.The conformation changes of plasmid DNA were assessed by agarose gel electrophoresis and the quantification was done using gel imaging system.Results The DNA damage induced by proton and 7Li heavy ion was much more serious as compared with that by 60Co γ-rays, and the vanillin derivative VND3207 could efficiently decrease the DNA damage induced by all three types of irradiation sources, which was expressed as a significantly reduced ratio of open circular form (OC) of plasmid DNA.The radioprotective effect of VND3207 increased with the increasing of drug concentration.The protective efficiencies of 200 μmol/L VND3207 were 85.3% (t =3.70,P =0.033), 73.3% (t = 10.58, P =0.017)and 80.4% (t =8.57,P =0.008)on DNA damage induction by 50 Gy of γ-rays, proton and 7Li heavy ion, respectively.It seemed that the radioprotection of VND3207 was more effective on DNA damage induced by high LET heavy ion than that by proton.Conclusions VND3207 has a protective effect against the genotoxicity of different LET ionizing radiation, especially for γ-rays and 7 Li heavy ion.
