中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
4期
234-236
,共3页
红斑狼疮,系统性%T淋巴细胞,辅助诱导%白细胞介素21
紅斑狼瘡,繫統性%T淋巴細胞,輔助誘導%白細胞介素21
홍반랑창,계통성%T림파세포,보조유도%백세포개소21
Lupus erythematosus,systemic%T-lymphocytes,helper-inducer%Interleukin-21
目的 观察系统性红斑狼疮(SLE)患儿外周血辅助性T17(Th17)细胞表达变化,探讨Th17细胞及其相关的细胞因子在SLE患儿发病中的作用.方法 以15名健康儿童为健康对照组,流式细胞术(FCM)检测25例SLE患儿(SLE组)外周血Th17细胞百分率,用酶联免疫吸附试验(ELISA)法检测外周血血浆白细胞介素(IL)-17、IL-21水平,分析Th17细胞与SLE活动度之间的关系.统计学处理采用t检验和Spearman相关性检验.结果 与健康对照组相比,SLE患儿外周血中CD3+CD8-IL-17+T细胞的频率[(1.24±0.64)%,(0.59±0.21)%],CD3+CD8-IL-21+T细胞的频率[(1.5±0.6)%,(0.8±0.4)%]明显增高,P均＜0.01;血浆中IL-17,IL-21水平明显升高(P＜0.01).CD3+CD8-IL-17+T细胞频率与疾病活动度相关(r=0.732,P＜0.01),CD3+CD8-IL-21+T细胞频率与疾病活动度无相关性(r=-0.002,P＞0.05).结论 Th17细胞及其相关细胞因子参与儿童SLE发病及病情发展.
目的 觀察繫統性紅斑狼瘡(SLE)患兒外週血輔助性T17(Th17)細胞錶達變化,探討Th17細胞及其相關的細胞因子在SLE患兒髮病中的作用.方法 以15名健康兒童為健康對照組,流式細胞術(FCM)檢測25例SLE患兒(SLE組)外週血Th17細胞百分率,用酶聯免疫吸附試驗(ELISA)法檢測外週血血漿白細胞介素(IL)-17、IL-21水平,分析Th17細胞與SLE活動度之間的關繫.統計學處理採用t檢驗和Spearman相關性檢驗.結果 與健康對照組相比,SLE患兒外週血中CD3+CD8-IL-17+T細胞的頻率[(1.24±0.64)%,(0.59±0.21)%],CD3+CD8-IL-21+T細胞的頻率[(1.5±0.6)%,(0.8±0.4)%]明顯增高,P均＜0.01;血漿中IL-17,IL-21水平明顯升高(P＜0.01).CD3+CD8-IL-17+T細胞頻率與疾病活動度相關(r=0.732,P＜0.01),CD3+CD8-IL-21+T細胞頻率與疾病活動度無相關性(r=-0.002,P＞0.05).結論 Th17細胞及其相關細胞因子參與兒童SLE髮病及病情髮展.
목적 관찰계통성홍반랑창(SLE)환인외주혈보조성T17(Th17)세포표체변화,탐토Th17세포급기상관적세포인자재SLE환인발병중적작용.방법 이15명건강인동위건강대조조,류식세포술(FCM)검측25례SLE환인(SLE조)외주혈Th17세포백분솔,용매련면역흡부시험(ELISA)법검측외주혈혈장백세포개소(IL)-17、IL-21수평,분석Th17세포여SLE활동도지간적관계.통계학처리채용t검험화Spearman상관성검험.결과 여건강대조조상비,SLE환인외주혈중CD3+CD8-IL-17+T세포적빈솔[(1.24±0.64)%,(0.59±0.21)%],CD3+CD8-IL-21+T세포적빈솔[(1.5±0.6)%,(0.8±0.4)%]명현증고,P균＜0.01;혈장중IL-17,IL-21수평명현승고(P＜0.01).CD3+CD8-IL-17+T세포빈솔여질병활동도상관(r=0.732,P＜0.01),CD3+CD8-IL-21+T세포빈솔여질병활동도무상관성(r=-0.002,P＞0.05).결론 Th17세포급기상관세포인자삼여인동SLE발병급병정발전.
Objective To investigate the Th17 cell expression in peripheral blood of childron with systemic lupus erythematosus (SLE) and explore the role of Th17 cells and the cytokines in the pathogenesis of SLE. Methods Twenty-five children with SLE were enrolled and 15 healthy children were controls. Flow cytometry (FCM) was employed to detect the expression of Th17 cells in peripheral blood of SLE children (SLE group, n=25), and IL-17, IL-21 levels in plasma were detected by ELISA. Two-independent sample t-test and Spearmen's test were used for correlation analysis. Results Compared with that of the control, the frequencies of CD3+CD8-IL-17+T[(1.24±0.64)% vs (0.59±0.21)%], CD3+CD8-IL-21+T cells[(1.5±0.6)%vs (0.8±0.4)% ] increased significantly in SLE patients (P＜0.01) and the plasma concentrations of IL-17, IL-21 were significantly higher (P＜0.01). The SLE activity was positively correlated with the frequencies of CD3+CD8-IL-17+T cells, but not with CD3+CD8-IL-21+T cells. Conclusion Th17 cells and the related cytokinesplay an important role in the pathogenesis of childhood SLE.
