中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
2期
117-120
,共4页
杨国嵘%黄高昇%薛永杰%贾兴民%何晓霞%郁荣%贺雪娇
楊國嶸%黃高昇%薛永傑%賈興民%何曉霞%鬱榮%賀雪嬌
양국영%황고승%설영걸%가흥민%하효하%욱영%하설교
人巨细胞病毒%腮腺导管上皮细胞%角蛋白8%角蛋白18
人巨細胞病毒%腮腺導管上皮細胞%角蛋白8%角蛋白18
인거세포병독%시선도관상피세포%각단백8%각단백18
Human cytomegalovirus%Parotid duct epithelial cell%Keratin 8%Keratin 18
目的 研究人巨细胞病毒(HCMV)感染对腮腺导管上皮细胞角蛋白K8和K18表达的影响.方法 用免疫组化方法研究腮腺巨细胞包涵体病(PCID)石蜡包埋组织中巨细胞病毒立即早期抗原和角蛋白K8、K18的表达.结果 PCID腮腺组织导管上皮出现包涵体巨细胞;包涵体巨细胞HCMV抗原DDG9/CCH2阳性;包涵体巨细胞角蛋白K8表达呈阴性,而K18表达呈强阳性.结论 HCMV感染腮腺导管上皮细胞诱发角蛋白K8降解,K18表达上调是一种反应性改变;单层上皮角蛋白网具有维持上皮细胞机械力学完整性的功能.
目的 研究人巨細胞病毒(HCMV)感染對腮腺導管上皮細胞角蛋白K8和K18錶達的影響.方法 用免疫組化方法研究腮腺巨細胞包涵體病(PCID)石蠟包埋組織中巨細胞病毒立即早期抗原和角蛋白K8、K18的錶達.結果 PCID腮腺組織導管上皮齣現包涵體巨細胞;包涵體巨細胞HCMV抗原DDG9/CCH2暘性;包涵體巨細胞角蛋白K8錶達呈陰性,而K18錶達呈彊暘性.結論 HCMV感染腮腺導管上皮細胞誘髮角蛋白K8降解,K18錶達上調是一種反應性改變;單層上皮角蛋白網具有維持上皮細胞機械力學完整性的功能.
목적 연구인거세포병독(HCMV)감염대시선도관상피세포각단백K8화K18표체적영향.방법 용면역조화방법연구시선거세포포함체병(PCID)석사포매조직중거세포병독립즉조기항원화각단백K8、K18적표체.결과 PCID시선조직도관상피출현포함체거세포;포함체거세포HCMV항원DDG9/CCH2양성;포함체거세포각단백K8표체정음성,이K18표체정강양성.결론 HCMV감염시선도관상피세포유발각단백K8강해,K18표체상조시일충반응성개변;단층상피각단백망구유유지상피세포궤계역학완정성적공능.
Objective To study the effect of human cytomegalovirus (HCMV) on expressions of K8 and K18 in duct epithelial cells of salivary gland. Methods The expressions of immediate early antigen of HCMV, K8 and K18 were detected by immunohistochemistry staining in tissues embedded in paraffin of parotid cytomegalic inclusion disease(PCID). Results Cytomegly bearing inclusion appeared in duct epithelium of PCID. DDG9/CCH2 antigen of HCMV was expressed in cytomegly bearing inclusion. K8 was negative in these cytomegly while K18 was intensively positive. Conclusion It is suggested that breaking down of K8 be induced in parotid duct epithelial cells infected by HCMV and that up-regulation of K18 may be a reactive change. Keratin network in simple epithelium functions to impart mechanical integrity to cells.
