CAJ | 학술논문

目的分析第3代和第4代HIV抗体诊断试剂间以及同一代各种试剂间的差异.方法用8种第3代试剂和4种第4代试剂分别检测989份人免疫缺陷病毒(HIV)阴性样品、185份HIV-1核酸阳性样品、HIV抗体第一代国际参考品以及9套BBI阳转血清,并判定检测结果.结果第4代试剂对HIV感染的检出时间普遍早于第3代试剂,但不同的第4代试剂的检出时间并不相同,不同的第3代试剂在HIV抗体的检出时间方面没有明显的差别.各种试剂检测不同基因型的能力不同,尤其国产试剂检测HIV-1 O组和HIV-2的能力普遍较差.结论本研究为试剂质量的进一步提高提供了实验数据.
목적 분석제3대화제4대HIV항체진단시제간이급동일대각충시제간적차이.방법 용8충제3대시제화4충제4대시제분별검측989빈인면역결함병독(HIV)음성양품、185빈HIV-1핵산양성양품、HIV항체제일대국제삼고품이급9투BBI양전혈청,병판정검측결과.결과 제4대시제대HIV감염적검출시간보편조우제3대시제,단불동적제4대시제적검출시간병불상동,불동적제3대시제재HIV항체적검출시간방면몰유명현적차별.각충시제검측불동기인형적능력불동,우기국산시제검측HIV-1 O조화HIV-2적능력보편교차.결론 본연구위시제질량적진일보제고제공료실험수거.
Objective To evaluate the differences between the third and the fourth generations of anti-HIV assays,and different kits within the same generation.Methods A total of 989 HIV-negative samples,185 samples positive for HIV-1 RNA.1st-generation international references of HIV antibodies and samples from 9 sets of BBI seroconversion panels were detected by 8 kits of the third generation and 4 kits of the fourth.Results The fourth generation kits can detect HIV infection earlier than the third generation kits.However,the detected days of HIV infection with different kits of the fourth generation were different whilst no significant difierences were found with difierent kits of the third generation.Furthermore,the capacity of detecting samples with different genotypes for different reagents was different,especially the capacity of domestic reagents on detecting HIV-1 O group and HIV-2 samples was relatively weak.Conclusion These data provided information to improve the quality of anti-HIV diagnostic reagents further.