中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2010年
3期
185-189
,共5页
彭素芳%王胜军%陈建国%戴晓莉%石燕%李雅贞%苏兆亮%薛渊%何志强%黄新祥%许化溪
彭素芳%王勝軍%陳建國%戴曉莉%石燕%李雅貞%囌兆亮%薛淵%何誌彊%黃新祥%許化溪
팽소방%왕성군%진건국%대효리%석연%리아정%소조량%설연%하지강%황신상%허화계
胃肿瘤%RORγt%FoxP3%Th17细胞%Treg细胞
胃腫瘤%RORγt%FoxP3%Th17細胞%Treg細胞
위종류%RORγt%FoxP3%Th17세포%Treg세포
Gastric neoplasms%RORγt%FoxP3%Th17 cell%Treg cell
目的 了解胃癌患者外周血Th17和Treg细胞的转录因子及其相关细胞因子的表达水平,探讨其在胃癌发展进程中的作用.方法 收集57例胃癌患者、31例胃部良性疾病患者和40例健康志愿者的外周血,采用实时荧光定量逆转录聚合酶链反应(QRT-PCR)技术,检测外周血单个核细胞中Th17和Treg细胞的特异性转录因子RORγt和FoxP3 mRNA的表达水平;采用酶联免疫吸附试验(ELISA),检测胃癌患者血浆中IL-17、IL-23、转化生长因子β(TGF-β)和IL-10的浓度.结果 胃癌组FoxP3和RORγt mRNA的表达水平分别为2.349±0.491和0.794±0.304,明显高于健康对照组和良性疾病组(均P<0.05),FoxP3/RORγt比值也高于健康对照组和良性疾病组(P<0.05),而良性疾病组与健康对照组差异无统计学意义(P>0.05).中晚期胃癌组的FoxP3/RORγt比值明显高于早期胃癌组(P<0.05).胃癌组血浆中IL-17、IL-23、TGF-β和IL-10的水平明显高于健康对照组(P<0.05),中晚期胃癌组TGF-β和IL-10水平明显高于早期胃癌组(P<0.05).结论 胃癌患者存在高Th17和Treg细胞水平,且随着病程的进展Treg细胞仍维持强势,FoxP3/RORγt比值明显增高.监测胃癌患者外周血Th17和Treg细胞的特异性转录因子和相关细胞因子水平,有助于对病程的判断.
目的 瞭解胃癌患者外週血Th17和Treg細胞的轉錄因子及其相關細胞因子的錶達水平,探討其在胃癌髮展進程中的作用.方法 收集57例胃癌患者、31例胃部良性疾病患者和40例健康誌願者的外週血,採用實時熒光定量逆轉錄聚閤酶鏈反應(QRT-PCR)技術,檢測外週血單箇覈細胞中Th17和Treg細胞的特異性轉錄因子RORγt和FoxP3 mRNA的錶達水平;採用酶聯免疫吸附試驗(ELISA),檢測胃癌患者血漿中IL-17、IL-23、轉化生長因子β(TGF-β)和IL-10的濃度.結果 胃癌組FoxP3和RORγt mRNA的錶達水平分彆為2.349±0.491和0.794±0.304,明顯高于健康對照組和良性疾病組(均P<0.05),FoxP3/RORγt比值也高于健康對照組和良性疾病組(P<0.05),而良性疾病組與健康對照組差異無統計學意義(P>0.05).中晚期胃癌組的FoxP3/RORγt比值明顯高于早期胃癌組(P<0.05).胃癌組血漿中IL-17、IL-23、TGF-β和IL-10的水平明顯高于健康對照組(P<0.05),中晚期胃癌組TGF-β和IL-10水平明顯高于早期胃癌組(P<0.05).結論 胃癌患者存在高Th17和Treg細胞水平,且隨著病程的進展Treg細胞仍維持彊勢,FoxP3/RORγt比值明顯增高.鑑測胃癌患者外週血Th17和Treg細胞的特異性轉錄因子和相關細胞因子水平,有助于對病程的判斷.
목적 료해위암환자외주혈Th17화Treg세포적전록인자급기상관세포인자적표체수평,탐토기재위암발전진정중적작용.방법 수집57례위암환자、31례위부량성질병환자화40례건강지원자적외주혈,채용실시형광정량역전록취합매련반응(QRT-PCR)기술,검측외주혈단개핵세포중Th17화Treg세포적특이성전록인자RORγt화FoxP3 mRNA적표체수평;채용매련면역흡부시험(ELISA),검측위암환자혈장중IL-17、IL-23、전화생장인자β(TGF-β)화IL-10적농도.결과 위암조FoxP3화RORγt mRNA적표체수평분별위2.349±0.491화0.794±0.304,명현고우건강대조조화량성질병조(균P<0.05),FoxP3/RORγt비치야고우건강대조조화량성질병조(P<0.05),이량성질병조여건강대조조차이무통계학의의(P>0.05).중만기위암조적FoxP3/RORγt비치명현고우조기위암조(P<0.05).위암조혈장중IL-17、IL-23、TGF-β화IL-10적수평명현고우건강대조조(P<0.05),중만기위암조TGF-β화IL-10수평명현고우조기위암조(P<0.05).결론 위암환자존재고Th17화Treg세포수평,차수착병정적진전Treg세포잉유지강세,FoxP3/RORγt비치명현증고.감측위암환자외주혈Th17화Treg세포적특이성전록인자화상관세포인자수평,유조우대병정적판단.
Objective To detect the expression levels of transcription factors and associated eytokines of Th17 and Treg cells in periphend blood mononuelear cells(PBMC)of patients with gastric cancer.and explore the possible pathological mechanism of these cells involved in the development of gastric cancer.Methods The mRNA levels of RORγt.FoxP3 in PBMC were determined by quantitative real-time PCR(QRT-PCR)from 57 patients with gastric cancer,31 patients with benign gastric illness and 40 healthy people.The concentration of IL-17,IL-23,TGF-β,IL-10 in plasma were detected by enzyme linked immtmosorbent assay(ELISA).Results Compared with healthy volunteers,patients with gastric cancer showed higher levels of RORγt and FoxP3 in PBMC(P<0.05).The ratio of FoxP3/RORγt in gastric cancer group was higher than that in the volunteer group and benign gastric illness group(P<0.05).The ratio of FoxP3/RORγt was higher in advanced disease than early disease(P<0.05).The expressions of IL-17,IL-23,TGF-β and IL-10 were higher in patients with gastric cancer than that in healthy volunteers(P<0.05).In addition,The expression of TGF-β and IL-10 were significantly increased in the advanced disease group than that in the early group(P<0.05),but IL-17 and IL-23 wag not significantly changed between the two groups(P>0.05).Conclusion There are higher levels of Th17 and Treg cells in gastric cancer patients.and it also shows a persistent predominant tendency of Treg cells and a reduced tendency of Th17cells in advanced disease.Detecting the expression of Th17/Treg transcription factor and related cytokines would contribute to the diagnosis and prediction of the disease development and prognosis.
