CAJ | 학술논문

用Inter-简单重复序列(ISSR)标记对樟子松东北分布区9个种源的135个个体遗传变异进行了检测.10个ISSR引物扩增共检测到108个位点,其中,多态位点百分率差异明显,范围在27%～54%之间,多态位点百分率最高的是红花尔基,最低的是卡伦山.物种水平的Shannon多样性指数(I)为0.1581,Nei基因多样度(h)为0.2393.遗传变异在种源间占39.65%,在种源内占60.35%.根据参试樟子松9个种源的聚类图,结果樟子松自然分布区划为2个种源区:大、小兴安岭种源区;呼伦贝尔种源区.图2表3参18.
용Inter-간단중복서렬(ISSR)표기대장자송동북분포구9개충원적135개개체유전변이진행료검측.10개ISSR인물확증공검측도108개위점,기중,다태위점백분솔차이명현,범위재27%～54%지간,다태위점백분솔최고적시홍화이기,최저적시잡륜산.물충수평적Shannon다양성지수(I)위0.1581,Nei기인다양도(h)위0.2393.유전변이재충원간점39.65%,재충원내점60.35%.근거삼시장자송9개충원적취류도,결과장자송자연분포구화위2개충원구:대、소흥안령충원구;호륜패이충원구.도2표3삼18.
Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among different provenances was evident, ranging from 27% to 54%, of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon's Information index (I) at species level was 0.1581 and Nei's gene diversity (h) was 0.2393. Coefficient of gene differentiation (GST) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram among pinus sylvestriv provenances, nine provenances were divided into two provenance areas, namely Daxing'an and Xiaoxing'an Mountains provenance area and Hulunbeier provenance area.